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[Effect of ubiquitin-proteasome pathway on TRAIL inducing apoptosis].
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Feb; 29(1):54-7.ZN

Abstract

OBJECTIVE

To determine the influence of ubiquitin-proteasome in regulating the TRAIL-mediated apoptosis.

METHODS

RASF cells and SCID mice were divided into 4 groups treated with PBS,anti-DR5, lactacystin, and anti-DR5 plus lactacystin. The apoptosis of cells was detected with Hoechst 33342 and TUNEL. The effect of caspase inhibitor on cell apoptosis was analysed with Hoechst 33342 and Luminescent ATP Lite.

RESULTS

Block ubiquitin-proteasome with lactacystin, a highly specific and irreversible proteasome inhibitor, induced 95% cell apoptosis both in vitro and in vivo. In contrast, there was no apoptosis of cells after the treatment with anti-DR5 antibody or lactacystin alone.

CONCLUSION

The inhibition of ubiquitin-proteasome pathway can sensitize rheumatoid arthritis synovial fibroblast cells to TRAIL- induced apoptosis, and the activation of caspase 8 and caspase 4 is necessary in this process.

Authors+Show Affiliations

Department of Occupational and Environmental Health, School of Public Health, Central South University, Changsha 410078, China.

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

16137006

Citation

Yang, Xin-Wen. "[Effect of Ubiquitin-proteasome Pathway On TRAIL Inducing Apoptosis]." Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, vol. 29, no. 1, 2004, pp. 54-7.
Yang XW. [Effect of ubiquitin-proteasome pathway on TRAIL inducing apoptosis]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004;29(1):54-7.
Yang, X. W. (2004). [Effect of ubiquitin-proteasome pathway on TRAIL inducing apoptosis]. Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, 29(1), 54-7.
Yang XW. [Effect of Ubiquitin-proteasome Pathway On TRAIL Inducing Apoptosis]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004;29(1):54-7. PubMed PMID: 16137006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of ubiquitin-proteasome pathway on TRAIL inducing apoptosis]. A1 - Yang,Xin-Wen, PY - 2005/9/3/pubmed PY - 2006/9/20/medline PY - 2005/9/3/entrez SP - 54 EP - 7 JF - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences JO - Zhong Nan Da Xue Xue Bao Yi Xue Ban VL - 29 IS - 1 N2 - OBJECTIVE: To determine the influence of ubiquitin-proteasome in regulating the TRAIL-mediated apoptosis. METHODS: RASF cells and SCID mice were divided into 4 groups treated with PBS,anti-DR5, lactacystin, and anti-DR5 plus lactacystin. The apoptosis of cells was detected with Hoechst 33342 and TUNEL. The effect of caspase inhibitor on cell apoptosis was analysed with Hoechst 33342 and Luminescent ATP Lite. RESULTS: Block ubiquitin-proteasome with lactacystin, a highly specific and irreversible proteasome inhibitor, induced 95% cell apoptosis both in vitro and in vivo. In contrast, there was no apoptosis of cells after the treatment with anti-DR5 antibody or lactacystin alone. CONCLUSION: The inhibition of ubiquitin-proteasome pathway can sensitize rheumatoid arthritis synovial fibroblast cells to TRAIL- induced apoptosis, and the activation of caspase 8 and caspase 4 is necessary in this process. SN - 1672-7347 UR - https://www.unboundmedicine.com/medline/citation/16137006/[Effect_of_ubiquitin_proteasome_pathway_on_TRAIL_inducing_apoptosis]_ L2 - http://xbyxb.csu.edu.cn/xbwk/fileup/PDF/20040254.pdf DB - PRIME DP - Unbound Medicine ER -