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Dissemination of SHV-12 and CTX-M-type extended-spectrum beta-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea.
J Antimicrob Chemother. 2005 Oct; 56(4):698-702.JA

Abstract

OBJECTIVES

To assess the prevalence and genotypes of Ambler class A extended-spectrum beta-lactamases (ESBLs) in Korea.

METHODS

Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February-July 2003 were evaluated. Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the putative ESBL-producing strains were tested by the double-disc synergy method. Detection of genes encoding class A beta-lactamases was performed by PCR amplification, and the PCR products were subjected to direct sequencing.

RESULTS

The double-disc synergy test showed positive results in 9.3% (23/246) of E. coli and 23.0% (55/239) of K. pneumoniae isolates. The most prevalent types of Ambler class A ESBLs in E. coli isolates were CTX-M-15 (n = 4) and CTX-M-3 (n = 3), and those in K. pneumoniae isolates were SHV-12 (n = 30) and CTX-M-3 (n = 13). Two isolates produced both SHV-12 and GES-3, simultaneously.

CONCLUSIONS

CTX-M-type and/or SHV-12 ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.

Authors+Show Affiliations

Department of Laboratory Medicine, Keimyung University College of Medicine, Daegu.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16141280

Citation

Ryoo, Nam Hee, et al. "Dissemination of SHV-12 and CTX-M-type Extended-spectrum Beta-lactamases Among Clinical Isolates of Escherichia Coli and Klebsiella Pneumoniae and Emergence of GES-3 in Korea." The Journal of Antimicrobial Chemotherapy, vol. 56, no. 4, 2005, pp. 698-702.
Ryoo NH, Kim EC, Hong SG, et al. Dissemination of SHV-12 and CTX-M-type extended-spectrum beta-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea. J Antimicrob Chemother. 2005;56(4):698-702.
Ryoo, N. H., Kim, E. C., Hong, S. G., Park, Y. J., Lee, K., Bae, I. K., Song, E. H., & Jeong, S. H. (2005). Dissemination of SHV-12 and CTX-M-type extended-spectrum beta-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea. The Journal of Antimicrobial Chemotherapy, 56(4), 698-702.
Ryoo NH, et al. Dissemination of SHV-12 and CTX-M-type Extended-spectrum Beta-lactamases Among Clinical Isolates of Escherichia Coli and Klebsiella Pneumoniae and Emergence of GES-3 in Korea. J Antimicrob Chemother. 2005;56(4):698-702. PubMed PMID: 16141280.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissemination of SHV-12 and CTX-M-type extended-spectrum beta-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea. AU - Ryoo,Nam Hee, AU - Kim,Eui-Chong, AU - Hong,Seong Geun, AU - Park,Yeon Joon, AU - Lee,Kyungwon, AU - Bae,Il Kwon, AU - Song,Eun Hyang, AU - Jeong,Seok Hoon, Y1 - 2005/09/02/ PY - 2005/9/6/pubmed PY - 2006/1/13/medline PY - 2005/9/6/entrez SP - 698 EP - 702 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 56 IS - 4 N2 - OBJECTIVES: To assess the prevalence and genotypes of Ambler class A extended-spectrum beta-lactamases (ESBLs) in Korea. METHODS: Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February-July 2003 were evaluated. Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the putative ESBL-producing strains were tested by the double-disc synergy method. Detection of genes encoding class A beta-lactamases was performed by PCR amplification, and the PCR products were subjected to direct sequencing. RESULTS: The double-disc synergy test showed positive results in 9.3% (23/246) of E. coli and 23.0% (55/239) of K. pneumoniae isolates. The most prevalent types of Ambler class A ESBLs in E. coli isolates were CTX-M-15 (n = 4) and CTX-M-3 (n = 3), and those in K. pneumoniae isolates were SHV-12 (n = 30) and CTX-M-3 (n = 13). Two isolates produced both SHV-12 and GES-3, simultaneously. CONCLUSIONS: CTX-M-type and/or SHV-12 ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea. SN - 0305-7453 UR - https://www.unboundmedicine.com/medline/citation/16141280/Dissemination_of_SHV_12_and_CTX_M_type_extended_spectrum_beta_lactamases_among_clinical_isolates_of_Escherichia_coli_and_Klebsiella_pneumoniae_and_emergence_of_GES_3_in_Korea_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dki324 DB - PRIME DP - Unbound Medicine ER -