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Direct isolation of satellite cells for skeletal muscle regeneration.
Science. 2005 Sep 23; 309(5743):2064-7.Sci

Abstract

Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.

Authors+Show Affiliations

CNRS Unité de Recherche Associée 2578, Department of Developmental Biology, INSERM, Pasteur Institute, 75724 Paris Cedex 15, France. dmontarr@pasteur.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16141372

Citation

Montarras, Didier, et al. "Direct Isolation of Satellite Cells for Skeletal Muscle Regeneration." Science (New York, N.Y.), vol. 309, no. 5743, 2005, pp. 2064-7.
Montarras D, Morgan J, Collins C, et al. Direct isolation of satellite cells for skeletal muscle regeneration. Science. 2005;309(5743):2064-7.
Montarras, D., Morgan, J., Collins, C., Relaix, F., Zaffran, S., Cumano, A., Partridge, T., & Buckingham, M. (2005). Direct isolation of satellite cells for skeletal muscle regeneration. Science (New York, N.Y.), 309(5743), 2064-7.
Montarras D, et al. Direct Isolation of Satellite Cells for Skeletal Muscle Regeneration. Science. 2005 Sep 23;309(5743):2064-7. PubMed PMID: 16141372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct isolation of satellite cells for skeletal muscle regeneration. AU - Montarras,Didier, AU - Morgan,Jennifer, AU - Collins,Charlotte, AU - Relaix,Frédéric, AU - Zaffran,Stéphane, AU - Cumano,Ana, AU - Partridge,Terence, AU - Buckingham,Margaret, Y1 - 2005/09/01/ PY - 2005/9/6/pubmed PY - 2005/10/21/medline PY - 2005/9/6/entrez SP - 2064 EP - 7 JF - Science (New York, N.Y.) JO - Science VL - 309 IS - 5743 N2 - Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity. SN - 1095-9203 UR - https://www.unboundmedicine.com/medline/citation/16141372/Direct_isolation_of_satellite_cells_for_skeletal_muscle_regeneration_ L2 - http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=16141372 DB - PRIME DP - Unbound Medicine ER -