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Adsorptive cathodic stripping voltammetric assay of the estrogen drug ethinylestradiol in pharmaceutical formulation and human plasma at a mercury electrode.
J Pharm Biomed Anal. 2006 Feb 13; 40(2):255-61.JP

Abstract

The electroreduction of ethinylestradiol at the hanging mercury drop electrode in the Britton-Robinson universal buffer of pH 2-11 was studied and its interfacial adsorptive character onto the mercury electrode surface was identified. A validated simple, rapid, sensitive, specific, precise and inexpensive square-wave voltammetric procedure is described for the determination of ethinylestradiol following its accumulation onto a hanging mercury drop electrode in a Britton-Robinson universal buffer of pH 7. The optimal procedural conditions were: accumulation potential E(acc)=-0.7 V versus Ag/AgCl/KCl(s), accumulation duration=60s, pulse-amplitude=70 mV, scan increment=10 mV and frequency=120 Hz. Limits of detection (LOD) and quantification (LOQ) of 5.9x10(-10)M and 1.9x10(-9)M bulk ethinylestradiol, respectively, were achieved. The proposed procedure was successfully applied to the quantification of ethinylestradiol in pharmaceutical formulation (Ethinyl-oestradiol tablets) and in human serum and plasma without the necessity for sample pretreatments and/or time-consuming extraction or evaporation steps prior to the analysis. LOD of 8.7x10(-10)M and 3x10(-9)M and LOQ of 2.9x10(-9)M and 1x10(-8)M of ethinylestradiol were achieved in human serum and plasma, respectively.

Authors+Show Affiliations

Department of Chemistry, Faculty of Science, Tanta University, 31527 Tanta, Egypt. enass@hotmail.co.ukNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16146680

Citation

Ghoneim, E M., et al. "Adsorptive Cathodic Stripping Voltammetric Assay of the Estrogen Drug Ethinylestradiol in Pharmaceutical Formulation and Human Plasma at a Mercury Electrode." Journal of Pharmaceutical and Biomedical Analysis, vol. 40, no. 2, 2006, pp. 255-61.
Ghoneim EM, El-Desoky HS, Ghoneim MM. Adsorptive cathodic stripping voltammetric assay of the estrogen drug ethinylestradiol in pharmaceutical formulation and human plasma at a mercury electrode. J Pharm Biomed Anal. 2006;40(2):255-61.
Ghoneim, E. M., El-Desoky, H. S., & Ghoneim, M. M. (2006). Adsorptive cathodic stripping voltammetric assay of the estrogen drug ethinylestradiol in pharmaceutical formulation and human plasma at a mercury electrode. Journal of Pharmaceutical and Biomedical Analysis, 40(2), 255-61.
Ghoneim EM, El-Desoky HS, Ghoneim MM. Adsorptive Cathodic Stripping Voltammetric Assay of the Estrogen Drug Ethinylestradiol in Pharmaceutical Formulation and Human Plasma at a Mercury Electrode. J Pharm Biomed Anal. 2006 Feb 13;40(2):255-61. PubMed PMID: 16146680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adsorptive cathodic stripping voltammetric assay of the estrogen drug ethinylestradiol in pharmaceutical formulation and human plasma at a mercury electrode. AU - Ghoneim,E M, AU - El-Desoky,H S, AU - Ghoneim,M M, Y1 - 2005/09/16/ PY - 2005/01/27/received PY - 2005/07/13/revised PY - 2005/07/15/accepted PY - 2005/9/9/pubmed PY - 2007/3/14/medline PY - 2005/9/9/entrez SP - 255 EP - 61 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 40 IS - 2 N2 - The electroreduction of ethinylestradiol at the hanging mercury drop electrode in the Britton-Robinson universal buffer of pH 2-11 was studied and its interfacial adsorptive character onto the mercury electrode surface was identified. A validated simple, rapid, sensitive, specific, precise and inexpensive square-wave voltammetric procedure is described for the determination of ethinylestradiol following its accumulation onto a hanging mercury drop electrode in a Britton-Robinson universal buffer of pH 7. The optimal procedural conditions were: accumulation potential E(acc)=-0.7 V versus Ag/AgCl/KCl(s), accumulation duration=60s, pulse-amplitude=70 mV, scan increment=10 mV and frequency=120 Hz. Limits of detection (LOD) and quantification (LOQ) of 5.9x10(-10)M and 1.9x10(-9)M bulk ethinylestradiol, respectively, were achieved. The proposed procedure was successfully applied to the quantification of ethinylestradiol in pharmaceutical formulation (Ethinyl-oestradiol tablets) and in human serum and plasma without the necessity for sample pretreatments and/or time-consuming extraction or evaporation steps prior to the analysis. LOD of 8.7x10(-10)M and 3x10(-9)M and LOQ of 2.9x10(-9)M and 1x10(-8)M of ethinylestradiol were achieved in human serum and plasma, respectively. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/16146680/Adsorptive_cathodic_stripping_voltammetric_assay_of_the_estrogen_drug_ethinylestradiol_in_pharmaceutical_formulation_and_human_plasma_at_a_mercury_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(05)00504-2 DB - PRIME DP - Unbound Medicine ER -