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Substance P activates p38 mitogen-activated protein kinase to promote IL-6 induction in human dental pulp fibroblasts.
Connect Tissue Res. 2005; 46(3):153-8.CT

Abstract

Substance P (SP) induces the expression of proinflammatory cytokines, such as interleukin (IL)-6, which are implicated in pulp inflammation. To determine the signal pathway of SP-induced IL-6, we examined the activities of the mitogen-activated protein kinases (MAPKs) in human dental pulp cell (PF-10) cultures. SP induced the phosphorylation of p38 MAPK within 5 min; this activation persisted for up to 40 min and was independent of the activation of extracellular signal-related kinases (ERK-1 and ERK-2) that were induced after SP stimulation of PF-10 cells. As shown by electrophoretic mobility shift assay p38 MAPK was not involved in SP-induced activation of nuclear factor-kappa B (NF-kappaB). However, p38 MAPK mediated SP-induced IL-6 production, as shown by the use of specific inhibitors of this kinase. Our results suggest that the activation of p38 MAPK is important for NF-kappaB-independent regulator of neurogenic inflammation in dental pulp tissues.

Authors+Show Affiliations

Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan. mas-tok@dentb.hal.kagoshima-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16147857

Citation

Tokuda, Masayuki, et al. "Substance P Activates P38 Mitogen-activated Protein Kinase to Promote IL-6 Induction in Human Dental Pulp Fibroblasts." Connective Tissue Research, vol. 46, no. 3, 2005, pp. 153-8.
Tokuda M, Miyamoto R, Sakuta T, et al. Substance P activates p38 mitogen-activated protein kinase to promote IL-6 induction in human dental pulp fibroblasts. Connect Tissue Res. 2005;46(3):153-8.
Tokuda, M., Miyamoto, R., Sakuta, T., Nagaoka, S., & Torii, M. (2005). Substance P activates p38 mitogen-activated protein kinase to promote IL-6 induction in human dental pulp fibroblasts. Connective Tissue Research, 46(3), 153-8.
Tokuda M, et al. Substance P Activates P38 Mitogen-activated Protein Kinase to Promote IL-6 Induction in Human Dental Pulp Fibroblasts. Connect Tissue Res. 2005;46(3):153-8. PubMed PMID: 16147857.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Substance P activates p38 mitogen-activated protein kinase to promote IL-6 induction in human dental pulp fibroblasts. AU - Tokuda,Masayuki, AU - Miyamoto,Rie, AU - Sakuta,Tetsuya, AU - Nagaoka,Shigetaka, AU - Torii,Mitsuo, PY - 2005/9/9/pubmed PY - 2005/10/15/medline PY - 2005/9/9/entrez SP - 153 EP - 8 JF - Connective tissue research JO - Connect Tissue Res VL - 46 IS - 3 N2 - Substance P (SP) induces the expression of proinflammatory cytokines, such as interleukin (IL)-6, which are implicated in pulp inflammation. To determine the signal pathway of SP-induced IL-6, we examined the activities of the mitogen-activated protein kinases (MAPKs) in human dental pulp cell (PF-10) cultures. SP induced the phosphorylation of p38 MAPK within 5 min; this activation persisted for up to 40 min and was independent of the activation of extracellular signal-related kinases (ERK-1 and ERK-2) that were induced after SP stimulation of PF-10 cells. As shown by electrophoretic mobility shift assay p38 MAPK was not involved in SP-induced activation of nuclear factor-kappa B (NF-kappaB). However, p38 MAPK mediated SP-induced IL-6 production, as shown by the use of specific inhibitors of this kinase. Our results suggest that the activation of p38 MAPK is important for NF-kappaB-independent regulator of neurogenic inflammation in dental pulp tissues. SN - 0300-8207 UR - https://www.unboundmedicine.com/medline/citation/16147857/Substance_P_activates_p38_mitogen_activated_protein_kinase_to_promote_IL_6_induction_in_human_dental_pulp_fibroblasts_ L2 - https://www.tandfonline.com/doi/full/10.1080/03008200500182490 DB - PRIME DP - Unbound Medicine ER -