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High concentrations of the melatonin metabolite, N1-acetyl-N2-formyl-5-methoxykynuramine, in cerebrospinal fluid of patients with meningitis: a possible immunomodulatory mechanism.
J Pineal Res. 2005 Oct; 39(3):302-6.JP

Abstract

We evaluated the presence of the melatonin metabolite N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK), in cerebrospinal fluid (CSF) of patients with viral meningitis (n = 20) and control samples (n = 8) and correlate AFMK levels with inflammatory markers such as cellularity, protein, tumor necrosis factor (TNF)-alpha, interleukin (IL)-8 and IL-1beta levels. A portion of the CSF was extracted with dichloromethane (1:5) and analyzed by high-performance liquid chromatography (HPLC) under standardized conditions for AFMK. AFMK was detected in 16 of 20 CSF samples of patients with viral meningitis; the concentration of AFMK was found to be above the quantification limit (50 nmol/L) in six of these samples. AFMK was not detected in any of the eight control samples. The samples were classified into groups according to AFMK levels: undetectable (<10 nmol/L, group I), detectable but below the quantification limit (< 50 nmol/L, group II), and quantified (>50 nmol/L, group III). Group II presented the highest levels of proteins and IL-8, whereas group III showed the lowest levels of the inflammatory parameters. This study supports our hypothesis that inflammation favors the formation of AFMK and that this compound has immunomodulatory activity in vivo.

Authors+Show Affiliations

Silva SO
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, Brazil.
Ximenes VF
No affiliation info available
Livramento JA
No affiliation info available
Catalani LH
No affiliation info available
Campa A
No affiliation info available

MeSH

Adjuvants, ImmunologicBiomarkersChromatography, High Pressure LiquidHumansKynuramineMelatoninMeningitis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16150112

Citation

Silva, Sueli de Oliveira, et al. "High Concentrations of the Melatonin Metabolite, N1-acetyl-N2-formyl-5-methoxykynuramine, in Cerebrospinal Fluid of Patients With Meningitis: a Possible Immunomodulatory Mechanism." Journal of Pineal Research, vol. 39, no. 3, 2005, pp. 302-6.
Silva SO, Ximenes VF, Livramento JA, et al. High concentrations of the melatonin metabolite, N1-acetyl-N2-formyl-5-methoxykynuramine, in cerebrospinal fluid of patients with meningitis: a possible immunomodulatory mechanism. J Pineal Res. 2005;39(3):302-6.
Silva, S. O., Ximenes, V. F., Livramento, J. A., Catalani, L. H., & Campa, A. (2005). High concentrations of the melatonin metabolite, N1-acetyl-N2-formyl-5-methoxykynuramine, in cerebrospinal fluid of patients with meningitis: a possible immunomodulatory mechanism. Journal of Pineal Research, 39(3), 302-6.
Silva SO, et al. High Concentrations of the Melatonin Metabolite, N1-acetyl-N2-formyl-5-methoxykynuramine, in Cerebrospinal Fluid of Patients With Meningitis: a Possible Immunomodulatory Mechanism. J Pineal Res. 2005;39(3):302-6. PubMed PMID: 16150112.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High concentrations of the melatonin metabolite, N1-acetyl-N2-formyl-5-methoxykynuramine, in cerebrospinal fluid of patients with meningitis: a possible immunomodulatory mechanism. AU - Silva,Sueli de Oliveira, AU - Ximenes,Valdecir F, AU - Livramento,José A, AU - Catalani,Luiz H, AU - Campa,Ana, PY - 2005/9/10/pubmed PY - 2006/7/19/medline PY - 2005/9/10/entrez SP - 302 EP - 6 JF - Journal of pineal research JO - J Pineal Res VL - 39 IS - 3 N2 - We evaluated the presence of the melatonin metabolite N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK), in cerebrospinal fluid (CSF) of patients with viral meningitis (n = 20) and control samples (n = 8) and correlate AFMK levels with inflammatory markers such as cellularity, protein, tumor necrosis factor (TNF)-alpha, interleukin (IL)-8 and IL-1beta levels. A portion of the CSF was extracted with dichloromethane (1:5) and analyzed by high-performance liquid chromatography (HPLC) under standardized conditions for AFMK. AFMK was detected in 16 of 20 CSF samples of patients with viral meningitis; the concentration of AFMK was found to be above the quantification limit (50 nmol/L) in six of these samples. AFMK was not detected in any of the eight control samples. The samples were classified into groups according to AFMK levels: undetectable (<10 nmol/L, group I), detectable but below the quantification limit (< 50 nmol/L, group II), and quantified (>50 nmol/L, group III). Group II presented the highest levels of proteins and IL-8, whereas group III showed the lowest levels of the inflammatory parameters. This study supports our hypothesis that inflammation favors the formation of AFMK and that this compound has immunomodulatory activity in vivo. SN - 0742-3098 UR - https://www.unboundmedicine.com/medline/citation/16150112/High_concentrations_of_the_melatonin_metabolite_N1_acetyl_N2_formyl_5_methoxykynuramine_in_cerebrospinal_fluid_of_patients_with_meningitis:_a_possible_immunomodulatory_mechanism_ DB - PRIME DP - Unbound Medicine ER -