Tags

Type your tag names separated by a space and hit enter

Simultaneous enantioselective determination of amphetamine and congeners in hair specimens by negative chemical ionization gas chromatography-mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Oct 15; 825(1):57-62.JC

Abstract

Enantioselective quantification of amphetamine (AM), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyethylamphetamine (MDEA) enantiomers in hair using gas chromatography-mass spectrometry (GC-MS) is described. Hair specimens were digested with 1M sodium hydroxide at 100 degrees C for 30 min and extracted by a solid phase procedure using Cleanscreen ZSDAU020. Extracted analytes were derivatised with (S)-heptafluorobutyrylprolyl chloride and the resulting diastereoisomers were quantified by GC-MS operating in the negative chemical ionization mode. Extraction yields were between 73.0 and 97.9%. Limits of detection varied in the range of 2.1-45.9 pg/mg hair, whereas the lowest limits of quantification varied between 4.3 and 91.8 pg/mg hair. Intra- and inter-assay precision and respective accuracy were acceptable. The enantiomeric ratios (R versus S) of AM, MA, MDA, MDMA and MDEA were determined in hair from suspected amphetamine abusers. Only MA and AM enantiomers were detectable in this collective and the quantification data showed in most cases higher concentrations of (R)-MA and (R)-AM than those of the corresponding (S)-enantiomers.

Authors+Show Affiliations

National Laboratory of Health, Toxicology Division, University of Luxembourg, CRP-Santé, 162A, Avenue de la Faïencerie, Luxembourg L-1511, Luxembourg. martins@cu.luNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

16154523

Citation

Martins, Liliane, et al. "Simultaneous Enantioselective Determination of Amphetamine and Congeners in Hair Specimens By Negative Chemical Ionization Gas Chromatography-mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 825, no. 1, 2005, pp. 57-62.
Martins L, Yegles M, Chung H, et al. Simultaneous enantioselective determination of amphetamine and congeners in hair specimens by negative chemical ionization gas chromatography-mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2005;825(1):57-62.
Martins, L., Yegles, M., Chung, H., & Wennig, R. (2005). Simultaneous enantioselective determination of amphetamine and congeners in hair specimens by negative chemical ionization gas chromatography-mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 825(1), 57-62.
Martins L, et al. Simultaneous Enantioselective Determination of Amphetamine and Congeners in Hair Specimens By Negative Chemical Ionization Gas Chromatography-mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Oct 15;825(1):57-62. PubMed PMID: 16154523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous enantioselective determination of amphetamine and congeners in hair specimens by negative chemical ionization gas chromatography-mass spectrometry. AU - Martins,Liliane, AU - Yegles,Michel, AU - Chung,Heesun, AU - Wennig,Robert, Y1 - 2005/01/27/ PY - 2004/10/16/received PY - 2004/12/20/revised PY - 2004/12/24/accepted PY - 2005/9/13/pubmed PY - 2005/11/1/medline PY - 2005/9/13/entrez SP - 57 EP - 62 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 825 IS - 1 N2 - Enantioselective quantification of amphetamine (AM), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyethylamphetamine (MDEA) enantiomers in hair using gas chromatography-mass spectrometry (GC-MS) is described. Hair specimens were digested with 1M sodium hydroxide at 100 degrees C for 30 min and extracted by a solid phase procedure using Cleanscreen ZSDAU020. Extracted analytes were derivatised with (S)-heptafluorobutyrylprolyl chloride and the resulting diastereoisomers were quantified by GC-MS operating in the negative chemical ionization mode. Extraction yields were between 73.0 and 97.9%. Limits of detection varied in the range of 2.1-45.9 pg/mg hair, whereas the lowest limits of quantification varied between 4.3 and 91.8 pg/mg hair. Intra- and inter-assay precision and respective accuracy were acceptable. The enantiomeric ratios (R versus S) of AM, MA, MDA, MDMA and MDEA were determined in hair from suspected amphetamine abusers. Only MA and AM enantiomers were detectable in this collective and the quantification data showed in most cases higher concentrations of (R)-MA and (R)-AM than those of the corresponding (S)-enantiomers. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16154523/Simultaneous_enantioselective_determination_of_amphetamine_and_congeners_in_hair_specimens_by_negative_chemical_ionization_gas_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(05)00037-1 DB - PRIME DP - Unbound Medicine ER -