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Urokinase (u-PA) is produced by collecting duct principal cells and is post-transcriptionally regulated by SV40 large-T, arginine vasopressin, and epidermal growth factor.
J Cell Physiol. 2006 Feb; 206(2):394-401.JC

Abstract

We have analyzed the expression and regulation of plasminogen activators (PA) in principal cells of the renal collecting duct. We used a rabbit principal cell line (RC.SVtsA58) infected with the temperature-sensitive SV40 strain tsA58. Transformed cells cultured at permissive temperature (33 degrees C) produced only tissue-type plasminogen activator (t-PA). Shifting the cells to nonpermissive temperature (39.5 degrees C) induced their differentiation and a marked increase in total fibrinolytic activity due to the induction of urokinase-type plasminogen activator (u-PA) synthesis and secretion. The effect on u-PA was post-transcriptional and it could be attributed to large-T inactivation at 39.5 degrees C since it was abolished by re-infecting the cells with wild-type SV40. Run-on assay and real-time RT-PCR of u-PA transcripts indicated that large-T altered post-transcriptional regulation. u-PA was also produced by primary cultures of collecting duct cells and was present in the rabbit urine. In the kidney, u-PA and its receptor (u-PAR) were almost exclusively expressed at the apex of collecting duct cells. We then analyzed the regulation of u-PA by arginine vasopressin (AVP) and epidermal growth factor (EGF), two key regulators of principal cell functions. We found that AVP and EGF, which have opposite hydro-osmotic effects in the collecting duct, also exhibited contrasted effects on u-PA synthesis in differentiated RC.SVtsA58 cells. EGF increased but AVP suppressed u-PA activity and protein, and these regulations occurred at post-transcriptional level. These results point to a physiological role of u-PA in principal cells of the renal collecting duct.

Authors+Show Affiliations

INSERM, U702, University Pierre et Marie Curie, and Hôpital Tenon, Paris, France. remi.piedagnel@tnn.ap-hop-paris.frNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16155905

Citation

Piedagnel, Rémi, et al. "Urokinase (u-PA) Is Produced By Collecting Duct Principal Cells and Is Post-transcriptionally Regulated By SV40 large-T, Arginine Vasopressin, and Epidermal Growth Factor." Journal of Cellular Physiology, vol. 206, no. 2, 2006, pp. 394-401.
Piedagnel R, Tiger Y, Lelongt B, et al. Urokinase (u-PA) is produced by collecting duct principal cells and is post-transcriptionally regulated by SV40 large-T, arginine vasopressin, and epidermal growth factor. J Cell Physiol. 2006;206(2):394-401.
Piedagnel, R., Tiger, Y., Lelongt, B., & Ronco, P. M. (2006). Urokinase (u-PA) is produced by collecting duct principal cells and is post-transcriptionally regulated by SV40 large-T, arginine vasopressin, and epidermal growth factor. Journal of Cellular Physiology, 206(2), 394-401.
Piedagnel R, et al. Urokinase (u-PA) Is Produced By Collecting Duct Principal Cells and Is Post-transcriptionally Regulated By SV40 large-T, Arginine Vasopressin, and Epidermal Growth Factor. J Cell Physiol. 2006;206(2):394-401. PubMed PMID: 16155905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Urokinase (u-PA) is produced by collecting duct principal cells and is post-transcriptionally regulated by SV40 large-T, arginine vasopressin, and epidermal growth factor. AU - Piedagnel,Rémi, AU - Tiger,Yoann, AU - Lelongt,Brigitte, AU - Ronco,Pierre M, PY - 2005/9/13/pubmed PY - 2006/3/11/medline PY - 2005/9/13/entrez SP - 394 EP - 401 JF - Journal of cellular physiology JO - J Cell Physiol VL - 206 IS - 2 N2 - We have analyzed the expression and regulation of plasminogen activators (PA) in principal cells of the renal collecting duct. We used a rabbit principal cell line (RC.SVtsA58) infected with the temperature-sensitive SV40 strain tsA58. Transformed cells cultured at permissive temperature (33 degrees C) produced only tissue-type plasminogen activator (t-PA). Shifting the cells to nonpermissive temperature (39.5 degrees C) induced their differentiation and a marked increase in total fibrinolytic activity due to the induction of urokinase-type plasminogen activator (u-PA) synthesis and secretion. The effect on u-PA was post-transcriptional and it could be attributed to large-T inactivation at 39.5 degrees C since it was abolished by re-infecting the cells with wild-type SV40. Run-on assay and real-time RT-PCR of u-PA transcripts indicated that large-T altered post-transcriptional regulation. u-PA was also produced by primary cultures of collecting duct cells and was present in the rabbit urine. In the kidney, u-PA and its receptor (u-PAR) were almost exclusively expressed at the apex of collecting duct cells. We then analyzed the regulation of u-PA by arginine vasopressin (AVP) and epidermal growth factor (EGF), two key regulators of principal cell functions. We found that AVP and EGF, which have opposite hydro-osmotic effects in the collecting duct, also exhibited contrasted effects on u-PA synthesis in differentiated RC.SVtsA58 cells. EGF increased but AVP suppressed u-PA activity and protein, and these regulations occurred at post-transcriptional level. These results point to a physiological role of u-PA in principal cells of the renal collecting duct. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/16155905/Urokinase__u_PA__is_produced_by_collecting_duct_principal_cells_and_is_post_transcriptionally_regulated_by_SV40_large_T_arginine_vasopressin_and_epidermal_growth_factor_ DB - PRIME DP - Unbound Medicine ER -