Tags

Type your tag names separated by a space and hit enter

Paraquat induces selective dopaminergic nigrostriatal degeneration in aging C57BL/6 mice.
Chin Med J (Engl). 2005 Aug 20; 118(16):1357-61.CM

Abstract

BACKGROUND

Paraquat (PQ; 1, 1'-dimethyl-4, 4'-bipyridinium), a widely used herbicide that is structurally similar to the known dopaminergic neurotoxicant MPTP (1-methyl-1, 2, 3, 6-tetrahydropyridine), has been suggested as a potential etiologic factor for the development of Parkinson's disease (PD). Aging is an accepted risk factor for idiopathic Parkinson's disease. The aim of this study was to test the hypothesis that paraquat could induce PD-like nigrostriatal dopaminergic degeneration in aging C57BL/6 mice.

METHODS

Senile male C57BL/6 mice were intraperitoneally injected with either saline or PQ at 2-day intervals for a total of 10 doses. Locomotor activity and performance on the pole test were measured 7 days after the last injection and animals were sacrificed one day later. Level of dopamine (DA) and its metabolites levels in the striatum were measured by high-performance liquid chromatography with an electrochemical detector (HPLC-ECD), and numbers of tyrosine hydroxylase (TH) positive neurons were estimated using immunohistochemistry.

RESULTS

Locomotor activities were significantly decreased and the behavioral performance on the pole test were significantly impaired in the PQ treated group. Level of DA and its metabolites levels in the striatum were declined by 8 days after the last injection. Immunohistochemical analyses showed that PQ was associated with a reduction in numbers of tyrosine hydroxylase positive neurons.

CONCLUSIONS

Long-term repeated exposes to PQ can selectively impair the nigrostriatal dopaminergic system of senile mice, suggesting that PQ could play an important role in the pathogenesis of Parkinson's disease (PD). Our results also validate a novel model of PD induced by exposure to a toxic environmental agent.

Authors+Show Affiliations

Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16157030

Citation

Li, Xia, et al. "Paraquat Induces Selective Dopaminergic Nigrostriatal Degeneration in Aging C57BL/6 Mice." Chinese Medical Journal, vol. 118, no. 16, 2005, pp. 1357-61.
Li X, Yin J, Cheng CM, et al. Paraquat induces selective dopaminergic nigrostriatal degeneration in aging C57BL/6 mice. Chin Med J (Engl). 2005;118(16):1357-61.
Li, X., Yin, J., Cheng, C. M., Sun, J. L., Li, Z., & Wu, Y. L. (2005). Paraquat induces selective dopaminergic nigrostriatal degeneration in aging C57BL/6 mice. Chinese Medical Journal, 118(16), 1357-61.
Li X, et al. Paraquat Induces Selective Dopaminergic Nigrostriatal Degeneration in Aging C57BL/6 Mice. Chin Med J (Engl). 2005 Aug 20;118(16):1357-61. PubMed PMID: 16157030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Paraquat induces selective dopaminergic nigrostriatal degeneration in aging C57BL/6 mice. AU - Li,Xia, AU - Yin,Jun, AU - Cheng,Chun-mei, AU - Sun,Jin-lai, AU - Li,Zheng, AU - Wu,Ying-liang, PY - 2005/9/15/pubmed PY - 2005/10/12/medline PY - 2005/9/15/entrez SP - 1357 EP - 61 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 118 IS - 16 N2 - BACKGROUND: Paraquat (PQ; 1, 1'-dimethyl-4, 4'-bipyridinium), a widely used herbicide that is structurally similar to the known dopaminergic neurotoxicant MPTP (1-methyl-1, 2, 3, 6-tetrahydropyridine), has been suggested as a potential etiologic factor for the development of Parkinson's disease (PD). Aging is an accepted risk factor for idiopathic Parkinson's disease. The aim of this study was to test the hypothesis that paraquat could induce PD-like nigrostriatal dopaminergic degeneration in aging C57BL/6 mice. METHODS: Senile male C57BL/6 mice were intraperitoneally injected with either saline or PQ at 2-day intervals for a total of 10 doses. Locomotor activity and performance on the pole test were measured 7 days after the last injection and animals were sacrificed one day later. Level of dopamine (DA) and its metabolites levels in the striatum were measured by high-performance liquid chromatography with an electrochemical detector (HPLC-ECD), and numbers of tyrosine hydroxylase (TH) positive neurons were estimated using immunohistochemistry. RESULTS: Locomotor activities were significantly decreased and the behavioral performance on the pole test were significantly impaired in the PQ treated group. Level of DA and its metabolites levels in the striatum were declined by 8 days after the last injection. Immunohistochemical analyses showed that PQ was associated with a reduction in numbers of tyrosine hydroxylase positive neurons. CONCLUSIONS: Long-term repeated exposes to PQ can selectively impair the nigrostriatal dopaminergic system of senile mice, suggesting that PQ could play an important role in the pathogenesis of Parkinson's disease (PD). Our results also validate a novel model of PD induced by exposure to a toxic environmental agent. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/16157030/Paraquat_induces_selective_dopaminergic_nigrostriatal_degeneration_in_aging_C57BL/6_mice_ DB - PRIME DP - Unbound Medicine ER -