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Cross-neutralization of the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms by antisera against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom.
Toxicon. 2005 Nov; 46(6):604-11.T

Abstract

We have previously demonstrated that rabbit antisera raised against crotoxin from Crotalus durissus cascavella venom (cdc-crotoxin) and its PLA2 (cdc-PLA2) neutralized the neurotoxicity of this venom and its crotoxin. In this study, we examined the ability of these antisera to neutralize the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms and their major toxins, cdt-crotoxin and bothropstoxin-I (BthTX-I), respectively, in mouse isolated phrenic nerve-diaphragm preparations. Immunoblotting showed that antiserum to cdc-crotoxin recognized cdt-crotoxin and BthTX-I, while antiserum to cdc-PLA2 recognized cdt-PLA2 and BthTX-I. ELISA corroborated this cross-reactivity. Antiserum to cdc-crotoxin prevented the neuromuscular blockade caused by C. d. terrificus venom and its crotoxin at a venom/crotoxin:antiserum ratio of 1:3. Antiserum to cdc-PLA2 also neutralized the neuromuscular blockade caused by C. d. terrificus venom or its crotoxin at venom or toxin:antiserum ratios of 1:3 and 1:1, respectively. The neuromuscular blockade caused by B. jararacussu venom and BthTX-I was also neutralized by the antisera to cdc-crotoxin and cdc-PLA2 at a venom/toxin:antiserum ratio of 1:10 for both. Commercial equine antivenom raised against C. d. terrificus venom was effective in preventing the neuromuscular blockade typical of B. jararacussu venom (venom:antivenom ratio of 1:2), whereas for BthTX-I the ratio was 1:10. These results show that antiserum produced against PLA2, the major toxin in C. durissus cascavella venom, efficiently neutralized the neurotoxicity of C. d. terrificus and B. jararacussu venoms and their PLA2 toxins.

Authors+Show Affiliations

Beghini DG
Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, CEP 13083-970 Campinas, SP, Brazil.
da Cruz-Höfling MA
No affiliation info available
Randazzo-Moura P
No affiliation info available
Rodrigues-Simioni L
No affiliation info available
Novello JC
No affiliation info available
Hyslop S
No affiliation info available
Marangoni S
No affiliation info available

MeSH

Analysis of VarianceAnimalsAntiveninsBothropsChromatography, AffinityCross ReactionsCrotalid VenomsCrotalusCrotoxinEnzyme-Linked Immunosorbent AssayImmune SeraImmunoblottingMusclesNeuromuscular JunctionNeurotoxinsNeutralization TestsPhospholipases APhospholipases A2Rabbits

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16157360

Citation

Beghini, Daniela G., et al. "Cross-neutralization of the Neurotoxicity of Crotalus Durissus Terrificus and Bothrops Jararacussu Venoms By Antisera Against Crotoxin and Phospholipase A2 From Crotalus Durissus Cascavella Venom." Toxicon : Official Journal of the International Society On Toxinology, vol. 46, no. 6, 2005, pp. 604-11.
Beghini DG, da Cruz-Höfling MA, Randazzo-Moura P, et al. Cross-neutralization of the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms by antisera against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom. Toxicon. 2005;46(6):604-11.
Beghini, D. G., da Cruz-Höfling, M. A., Randazzo-Moura, P., Rodrigues-Simioni, L., Novello, J. C., Hyslop, S., & Marangoni, S. (2005). Cross-neutralization of the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms by antisera against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom. Toxicon : Official Journal of the International Society On Toxinology, 46(6), 604-11.
Beghini DG, et al. Cross-neutralization of the Neurotoxicity of Crotalus Durissus Terrificus and Bothrops Jararacussu Venoms By Antisera Against Crotoxin and Phospholipase A2 From Crotalus Durissus Cascavella Venom. Toxicon. 2005;46(6):604-11. PubMed PMID: 16157360.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cross-neutralization of the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms by antisera against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom. AU - Beghini,Daniela G, AU - da Cruz-Höfling,Maria Alice, AU - Randazzo-Moura,Priscila, AU - Rodrigues-Simioni,Léa, AU - Novello,José Camilo, AU - Hyslop,Stephen, AU - Marangoni,Sérgio, Y1 - 2005/09/12/ PY - 2004/12/14/received PY - 2005/06/23/revised PY - 2005/06/24/accepted PY - 2005/9/15/pubmed PY - 2006/6/6/medline PY - 2005/9/15/entrez SP - 604 EP - 11 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 46 IS - 6 N2 - We have previously demonstrated that rabbit antisera raised against crotoxin from Crotalus durissus cascavella venom (cdc-crotoxin) and its PLA2 (cdc-PLA2) neutralized the neurotoxicity of this venom and its crotoxin. In this study, we examined the ability of these antisera to neutralize the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms and their major toxins, cdt-crotoxin and bothropstoxin-I (BthTX-I), respectively, in mouse isolated phrenic nerve-diaphragm preparations. Immunoblotting showed that antiserum to cdc-crotoxin recognized cdt-crotoxin and BthTX-I, while antiserum to cdc-PLA2 recognized cdt-PLA2 and BthTX-I. ELISA corroborated this cross-reactivity. Antiserum to cdc-crotoxin prevented the neuromuscular blockade caused by C. d. terrificus venom and its crotoxin at a venom/crotoxin:antiserum ratio of 1:3. Antiserum to cdc-PLA2 also neutralized the neuromuscular blockade caused by C. d. terrificus venom or its crotoxin at venom or toxin:antiserum ratios of 1:3 and 1:1, respectively. The neuromuscular blockade caused by B. jararacussu venom and BthTX-I was also neutralized by the antisera to cdc-crotoxin and cdc-PLA2 at a venom/toxin:antiserum ratio of 1:10 for both. Commercial equine antivenom raised against C. d. terrificus venom was effective in preventing the neuromuscular blockade typical of B. jararacussu venom (venom:antivenom ratio of 1:2), whereas for BthTX-I the ratio was 1:10. These results show that antiserum produced against PLA2, the major toxin in C. durissus cascavella venom, efficiently neutralized the neurotoxicity of C. d. terrificus and B. jararacussu venoms and their PLA2 toxins. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/16157360/Cross_neutralization_of_the_neurotoxicity_of_Crotalus_durissus_terrificus_and_Bothrops_jararacussu_venoms_by_antisera_against_crotoxin_and_phospholipase_A2_from_Crotalus_durissus_cascavella_venom_ DB - PRIME DP - Unbound Medicine ER -