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Identification and mapping of H32, a new wheat gene conferring resistance to Hessian fly.
Theor Appl Genet. 2005 Oct; 111(6):1167-73.TA

Abstract

H32 is a newly identified gene that confers resistance to the highly pervasive Biotype L of the Hessian fly [ Mayetiola destructor (Say)]. The gene was identified in a synthetic amphihexaploid wheat, W-7984, that was constructed from the durum 'Altar 84' and Aegilops tauschii. This synthetic wheat is one of the parents of the marker-rich ITMI population, which consists of 150 recombinant inbred lines (RILs) derived by single-seed descent from a cross with 'Opata 85'. Linkage analysis of the H32 locus in the ITMI population placed the gene between flanking microsatellite (SSR) markers, Xgwm3 and Xcfd223, at distances of 3.7 and 1.7 cM, respectively, on the long arm of chromosome 3D. The Xgwm3 primers amplified codominant SSR alleles, a 72 bp fragment linked in coupling to the resistance allele and an 84 bp fragment linked in repulsion. Primers for the SSR Xcfd223 amplified a 153 bp fragment from the resistant Synthetic parent and a 183 bp fragment from the susceptible Opata line. Deletion mapping of the flanking Xgwm3 and Xcfd223 markers located them within the 3DL-3 deletion on the distal 19% of the long arm of chromosome 3D. This location is at least 20 cM proximal to the reported 3DL location of H24, a gene that confers resistance to Biotype D of the Hessian fly. Tight linkage of the markers will provide a means of detecting H32 presence in marker-assisted selection and gene pyramiding as an effective strategy for extending durability of deployed resistance.

Authors+Show Affiliations

Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16160821

Citation

Sardesai, N, et al. "Identification and Mapping of H32, a New Wheat Gene Conferring Resistance to Hessian Fly." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 111, no. 6, 2005, pp. 1167-73.
Sardesai N, Nemacheck JA, Subramanyam S, et al. Identification and mapping of H32, a new wheat gene conferring resistance to Hessian fly. Theor Appl Genet. 2005;111(6):1167-73.
Sardesai, N., Nemacheck, J. A., Subramanyam, S., & Williams, C. E. (2005). Identification and mapping of H32, a new wheat gene conferring resistance to Hessian fly. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 111(6), 1167-73.
Sardesai N, et al. Identification and Mapping of H32, a New Wheat Gene Conferring Resistance to Hessian Fly. Theor Appl Genet. 2005;111(6):1167-73. PubMed PMID: 16160821.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and mapping of H32, a new wheat gene conferring resistance to Hessian fly. AU - Sardesai,N, AU - Nemacheck,J A, AU - Subramanyam,S, AU - Williams,C E, Y1 - 2005/10/11/ PY - 2005/05/06/received PY - 2005/07/12/accepted PY - 2005/9/15/pubmed PY - 2006/6/6/medline PY - 2005/9/15/entrez SP - 1167 EP - 73 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 111 IS - 6 N2 - H32 is a newly identified gene that confers resistance to the highly pervasive Biotype L of the Hessian fly [ Mayetiola destructor (Say)]. The gene was identified in a synthetic amphihexaploid wheat, W-7984, that was constructed from the durum 'Altar 84' and Aegilops tauschii. This synthetic wheat is one of the parents of the marker-rich ITMI population, which consists of 150 recombinant inbred lines (RILs) derived by single-seed descent from a cross with 'Opata 85'. Linkage analysis of the H32 locus in the ITMI population placed the gene between flanking microsatellite (SSR) markers, Xgwm3 and Xcfd223, at distances of 3.7 and 1.7 cM, respectively, on the long arm of chromosome 3D. The Xgwm3 primers amplified codominant SSR alleles, a 72 bp fragment linked in coupling to the resistance allele and an 84 bp fragment linked in repulsion. Primers for the SSR Xcfd223 amplified a 153 bp fragment from the resistant Synthetic parent and a 183 bp fragment from the susceptible Opata line. Deletion mapping of the flanking Xgwm3 and Xcfd223 markers located them within the 3DL-3 deletion on the distal 19% of the long arm of chromosome 3D. This location is at least 20 cM proximal to the reported 3DL location of H24, a gene that confers resistance to Biotype D of the Hessian fly. Tight linkage of the markers will provide a means of detecting H32 presence in marker-assisted selection and gene pyramiding as an effective strategy for extending durability of deployed resistance. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/16160821/Identification_and_mapping_of_H32_a_new_wheat_gene_conferring_resistance_to_Hessian_fly_ L2 - https://dx.doi.org/10.1007/s00122-005-0048-6 DB - PRIME DP - Unbound Medicine ER -