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In vitro antioxidant studies and free radical reactions of triphala, an ayurvedic formulation and its constituents.
Phytother Res. 2005 Jul; 19(7):582-6.PR

Abstract

The aqueous extract of the fruits of Emblica officinalis (T1), Terminalia chebula (T2) and Terminalia belerica (T3) and their equiproportional mixture triphala were evaluated for their in vitro antioxidant activity. gamma-Radiation induced strand break formation in plasmid DNA (pBR322) was effectively inhibited by triphala and its constituents in the concentration range 25-200 microg/mL with a percentage inhibition of T1 (30%-83%), T2 (21%-71%), T3 (8%-58%) and triphala (17%-63%). They also inhibited radiation induced lipid peroxidation in rat liver microsomes effectively with IC(50) values less than 15 microg/mL. The extracts were found to possess the ability to scavenge free radicals such as DPPH and superoxide. As the phenolic compounds present in these extracts are mostly responsible for their radical scavenging activity, the total phenolic contents present in these extracts were determined and expressed in terms of gallic acid equivalents and were found to vary from 33% to 44%. These studies revealed that all three constituents of triphala are active and they exhibit slightly different activities under different conditions. T1 shows greater efficiency in lipid peroxidation and plasmid DNA assay, while T2 has greater radical scavenging activity. Thus their mixture, triphala, is expected to be more efficient due to the combined activity of the individual components.

Authors+Show Affiliations

Radiation Chemistry and Chemical Dynamics Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400085, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16161061

Citation

Naik, G H., et al. "In Vitro Antioxidant Studies and Free Radical Reactions of Triphala, an Ayurvedic Formulation and Its Constituents." Phytotherapy Research : PTR, vol. 19, no. 7, 2005, pp. 582-6.
Naik GH, Priyadarsini KI, Bhagirathi RG, et al. In vitro antioxidant studies and free radical reactions of triphala, an ayurvedic formulation and its constituents. Phytother Res. 2005;19(7):582-6.
Naik, G. H., Priyadarsini, K. I., Bhagirathi, R. G., Mishra, B., Mishra, K. P., Banavalikar, M. M., & Mohan, H. (2005). In vitro antioxidant studies and free radical reactions of triphala, an ayurvedic formulation and its constituents. Phytotherapy Research : PTR, 19(7), 582-6.
Naik GH, et al. In Vitro Antioxidant Studies and Free Radical Reactions of Triphala, an Ayurvedic Formulation and Its Constituents. Phytother Res. 2005;19(7):582-6. PubMed PMID: 16161061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antioxidant studies and free radical reactions of triphala, an ayurvedic formulation and its constituents. AU - Naik,G H, AU - Priyadarsini,K I, AU - Bhagirathi,R G, AU - Mishra,B, AU - Mishra,K P, AU - Banavalikar,M M, AU - Mohan,Hari, PY - 2005/9/15/pubmed PY - 2005/12/13/medline PY - 2005/9/15/entrez SP - 582 EP - 6 JF - Phytotherapy research : PTR JO - Phytother Res VL - 19 IS - 7 N2 - The aqueous extract of the fruits of Emblica officinalis (T1), Terminalia chebula (T2) and Terminalia belerica (T3) and their equiproportional mixture triphala were evaluated for their in vitro antioxidant activity. gamma-Radiation induced strand break formation in plasmid DNA (pBR322) was effectively inhibited by triphala and its constituents in the concentration range 25-200 microg/mL with a percentage inhibition of T1 (30%-83%), T2 (21%-71%), T3 (8%-58%) and triphala (17%-63%). They also inhibited radiation induced lipid peroxidation in rat liver microsomes effectively with IC(50) values less than 15 microg/mL. The extracts were found to possess the ability to scavenge free radicals such as DPPH and superoxide. As the phenolic compounds present in these extracts are mostly responsible for their radical scavenging activity, the total phenolic contents present in these extracts were determined and expressed in terms of gallic acid equivalents and were found to vary from 33% to 44%. These studies revealed that all three constituents of triphala are active and they exhibit slightly different activities under different conditions. T1 shows greater efficiency in lipid peroxidation and plasmid DNA assay, while T2 has greater radical scavenging activity. Thus their mixture, triphala, is expected to be more efficient due to the combined activity of the individual components. SN - 0951-418X UR - https://www.unboundmedicine.com/medline/citation/16161061/In_vitro_antioxidant_studies_and_free_radical_reactions_of_triphala_an_ayurvedic_formulation_and_its_constituents_ L2 - https://doi.org/10.1002/ptr.1515 DB - PRIME DP - Unbound Medicine ER -