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Islet auto-transplantation into an omental or splenic site results in a normal beta cell but abnormal alpha cell response to mild non-insulin-induced hypoglycemia.
Am J Transplant. 2005 Oct; 5(10):2368-77.AJ

Abstract

The present studies were designed to determine if totally pancreatectomized dogs that underwent islet auto-transplantation retained a functional pancreatic counterregulatory response to mild non-insulin-induced hypoglycemia. Six dogs underwent total pancreatectomy followed by islet auto-transplantation to spleen or omentum. The animals recovered and fasting plasma glucose and insulin levels were normal. Each study consisted of a 40-min control and 2-h test period. At the onset of the test period, a glycogen phosphorylase inhibitor was administered to create mild hypoglycemia. Plasma glucose in the transplanted dogs fell from 120 +/- 4 to 80 +/- 3 mg/dL, similar to the minimum in control dogs without islet auto-transplantation (108 +/- 2 to 84 +/- 5 mg/dL). The fall in plasma insulin was similar in both groups. Glucagon, however, rose in response to hypoglycemia in the control dogs (Delta24 +/- 7 pg/mL; p < 0.05), but failed to rise significantly in the transplanted dogs (Delta9 +/- 6 pg/mL). In fact, only 1 of 7 control dogs failed to increase plasma glucagon by at least 25%, whereas 4 of 6 transplanted dogs failed to do so. In conclusion, in conscious dogs with successfully auto-transplanted islets, the beta cell response to mild non-insulin-induced hypoglycemia was normal, whereas the alpha cell response was not.

Authors+Show Affiliations

Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16162184

Citation

Gustavson, Stephanie M., et al. "Islet Auto-transplantation Into an Omental or Splenic Site Results in a Normal Beta Cell but Abnormal Alpha Cell Response to Mild Non-insulin-induced Hypoglycemia." American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, vol. 5, no. 10, 2005, pp. 2368-77.
Gustavson SM, Rajotte RV, Hunkeler D, et al. Islet auto-transplantation into an omental or splenic site results in a normal beta cell but abnormal alpha cell response to mild non-insulin-induced hypoglycemia. Am J Transplant. 2005;5(10):2368-77.
Gustavson, S. M., Rajotte, R. V., Hunkeler, D., Lakey, J. R., Edgerton, D. S., Neal, D. W., Snead, W. L., Penaloza, A. R., & Cherrington, A. D. (2005). Islet auto-transplantation into an omental or splenic site results in a normal beta cell but abnormal alpha cell response to mild non-insulin-induced hypoglycemia. American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 5(10), 2368-77.
Gustavson SM, et al. Islet Auto-transplantation Into an Omental or Splenic Site Results in a Normal Beta Cell but Abnormal Alpha Cell Response to Mild Non-insulin-induced Hypoglycemia. Am J Transplant. 2005;5(10):2368-77. PubMed PMID: 16162184.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Islet auto-transplantation into an omental or splenic site results in a normal beta cell but abnormal alpha cell response to mild non-insulin-induced hypoglycemia. AU - Gustavson,Stephanie M, AU - Rajotte,Ray V, AU - Hunkeler,David, AU - Lakey,Jonathan R T, AU - Edgerton,Dale S, AU - Neal,Doss W, AU - Snead,Wanda L, AU - Penaloza,Angelina R, AU - Cherrington,Alan D, PY - 2005/9/16/pubmed PY - 2006/1/5/medline PY - 2005/9/16/entrez SP - 2368 EP - 77 JF - American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons JO - Am J Transplant VL - 5 IS - 10 N2 - The present studies were designed to determine if totally pancreatectomized dogs that underwent islet auto-transplantation retained a functional pancreatic counterregulatory response to mild non-insulin-induced hypoglycemia. Six dogs underwent total pancreatectomy followed by islet auto-transplantation to spleen or omentum. The animals recovered and fasting plasma glucose and insulin levels were normal. Each study consisted of a 40-min control and 2-h test period. At the onset of the test period, a glycogen phosphorylase inhibitor was administered to create mild hypoglycemia. Plasma glucose in the transplanted dogs fell from 120 +/- 4 to 80 +/- 3 mg/dL, similar to the minimum in control dogs without islet auto-transplantation (108 +/- 2 to 84 +/- 5 mg/dL). The fall in plasma insulin was similar in both groups. Glucagon, however, rose in response to hypoglycemia in the control dogs (Delta24 +/- 7 pg/mL; p < 0.05), but failed to rise significantly in the transplanted dogs (Delta9 +/- 6 pg/mL). In fact, only 1 of 7 control dogs failed to increase plasma glucagon by at least 25%, whereas 4 of 6 transplanted dogs failed to do so. In conclusion, in conscious dogs with successfully auto-transplanted islets, the beta cell response to mild non-insulin-induced hypoglycemia was normal, whereas the alpha cell response was not. SN - 1600-6135 UR - https://www.unboundmedicine.com/medline/citation/16162184/Islet_auto_transplantation_into_an_omental_or_splenic_site_results_in_a_normal_beta_cell_but_abnormal_alpha_cell_response_to_mild_non_insulin_induced_hypoglycemia_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=1600-6135&amp;date=2005&amp;volume=5&amp;issue=10&amp;spage=2368 DB - PRIME DP - Unbound Medicine ER -