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Local change in phospholipid composition at the cleavage furrow is essential for completion of cytokinesis.
J Biol Chem 2005; 280(45):37901-7JB

Abstract

Cell division ends up with the membrane separation of two daughter cells, presumably by a membrane fusion that requires dynamic changes of the distribution and the composition of membrane lipids. We have previously shown that a membrane lipid phosphatidylethanolamine (PE) is exposed on the cell surface of the cleavage furrow during late cytokinesis and that this PE movement is involved in regulation of the contractile ring disassembly. Here we show that immobilization of cell surface PE by a PE-binding peptide blocks the RhoA inactivation in the late stage of cytokinesis. Phosphatidylinositol 4-phosphate 5-kinase (PIP5K), but not other RhoA effectors, is co-localized with RhoA in the peptide-treated cells. Indeed, PIP5K and its product phosphatidylinositol 4,5-bisphosphate (PI(4,5)P(2)) are localized to the cleavage furrow of normally dividing cells. Both overexpression of a kinase-deficient PIP5K mutant and microinjection of anti-PI(4,5)P(2) antibodies compromise cytokinesis by preventing local accumulation of PI(4,5)P(2) in the cleavage furrow. These findings demonstrate that the localized production of PI(4,5)P(2) is required for the proper completion of cytokinesis and that the possible formation of a unique lipid domain in the cleavage furrow membrane may play a crucial role in coordinating the contractile rearrangement with the membrane remodeling during late cytokinesis.

Authors+Show Affiliations

Institute for Chemical Research, Kyoto University, Uji, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16162509

Citation

Emoto, Kazuo, et al. "Local Change in Phospholipid Composition at the Cleavage Furrow Is Essential for Completion of Cytokinesis." The Journal of Biological Chemistry, vol. 280, no. 45, 2005, pp. 37901-7.
Emoto K, Inadome H, Kanaho Y, et al. Local change in phospholipid composition at the cleavage furrow is essential for completion of cytokinesis. J Biol Chem. 2005;280(45):37901-7.
Emoto, K., Inadome, H., Kanaho, Y., Narumiya, S., & Umeda, M. (2005). Local change in phospholipid composition at the cleavage furrow is essential for completion of cytokinesis. The Journal of Biological Chemistry, 280(45), pp. 37901-7.
Emoto K, et al. Local Change in Phospholipid Composition at the Cleavage Furrow Is Essential for Completion of Cytokinesis. J Biol Chem. 2005 Nov 11;280(45):37901-7. PubMed PMID: 16162509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Local change in phospholipid composition at the cleavage furrow is essential for completion of cytokinesis. AU - Emoto,Kazuo, AU - Inadome,Hironori, AU - Kanaho,Yasunori, AU - Narumiya,Shuh, AU - Umeda,Masato, Y1 - 2005/09/14/ PY - 2005/9/16/pubmed PY - 2006/1/13/medline PY - 2005/9/16/entrez SP - 37901 EP - 7 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 280 IS - 45 N2 - Cell division ends up with the membrane separation of two daughter cells, presumably by a membrane fusion that requires dynamic changes of the distribution and the composition of membrane lipids. We have previously shown that a membrane lipid phosphatidylethanolamine (PE) is exposed on the cell surface of the cleavage furrow during late cytokinesis and that this PE movement is involved in regulation of the contractile ring disassembly. Here we show that immobilization of cell surface PE by a PE-binding peptide blocks the RhoA inactivation in the late stage of cytokinesis. Phosphatidylinositol 4-phosphate 5-kinase (PIP5K), but not other RhoA effectors, is co-localized with RhoA in the peptide-treated cells. Indeed, PIP5K and its product phosphatidylinositol 4,5-bisphosphate (PI(4,5)P(2)) are localized to the cleavage furrow of normally dividing cells. Both overexpression of a kinase-deficient PIP5K mutant and microinjection of anti-PI(4,5)P(2) antibodies compromise cytokinesis by preventing local accumulation of PI(4,5)P(2) in the cleavage furrow. These findings demonstrate that the localized production of PI(4,5)P(2) is required for the proper completion of cytokinesis and that the possible formation of a unique lipid domain in the cleavage furrow membrane may play a crucial role in coordinating the contractile rearrangement with the membrane remodeling during late cytokinesis. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/16162509/Local_change_in_phospholipid_composition_at_the_cleavage_furrow_is_essential_for_completion_of_cytokinesis_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=16162509 DB - PRIME DP - Unbound Medicine ER -