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Molecular cloning and functional expression of d-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli.
FEMS Microbiol Lett. 2005 Nov 01; 252(1):35-42.FM

Abstract

A NADP-dependent d-arabitol dehydrogenase gene was cloned from Gluconobacter oxydans CGMCC 1.110 and functionally expressed in Escherichia coli. With d-arabitol as sole carbon source, E. coli transformants grew rapidly in minimal medium, and produced d-xylulose. The enzymatic properties of the 29kDa enzyme were documented. The DNA sequence surrounding the gene suggested that it is part of an operon with several components of a sugar alcohol transporter system, and the d-arabitol dehydrogenase gene belongs to the short-chain dehydrogenase family.

Authors+Show Affiliations

Center for Microbial Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16165327

Citation

Cheng, Hairong, et al. "Molecular Cloning and Functional Expression of D-arabitol Dehydrogenase Gene From Gluconobacter Oxydans in Escherichia Coli." FEMS Microbiology Letters, vol. 252, no. 1, 2005, pp. 35-42.
Cheng H, Jiang N, Shen A, et al. Molecular cloning and functional expression of d-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli. FEMS Microbiol Lett. 2005;252(1):35-42.
Cheng, H., Jiang, N., Shen, A., & Feng, Y. (2005). Molecular cloning and functional expression of d-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli. FEMS Microbiology Letters, 252(1), 35-42.
Cheng H, et al. Molecular Cloning and Functional Expression of D-arabitol Dehydrogenase Gene From Gluconobacter Oxydans in Escherichia Coli. FEMS Microbiol Lett. 2005 Nov 1;252(1):35-42. PubMed PMID: 16165327.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and functional expression of d-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli. AU - Cheng,Hairong, AU - Jiang,Ning, AU - Shen,An, AU - Feng,Youjun, Y1 - 2005/08/30/ PY - 2005/06/28/received PY - 2005/08/11/revised PY - 2005/08/16/accepted PY - 2005/9/17/pubmed PY - 2005/12/15/medline PY - 2005/9/17/entrez SP - 35 EP - 42 JF - FEMS microbiology letters JO - FEMS Microbiol. Lett. VL - 252 IS - 1 N2 - A NADP-dependent d-arabitol dehydrogenase gene was cloned from Gluconobacter oxydans CGMCC 1.110 and functionally expressed in Escherichia coli. With d-arabitol as sole carbon source, E. coli transformants grew rapidly in minimal medium, and produced d-xylulose. The enzymatic properties of the 29kDa enzyme were documented. The DNA sequence surrounding the gene suggested that it is part of an operon with several components of a sugar alcohol transporter system, and the d-arabitol dehydrogenase gene belongs to the short-chain dehydrogenase family. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/16165327/Molecular_cloning_and_functional_expression_of_d_arabitol_dehydrogenase_gene_from_Gluconobacter_oxydans_in_Escherichia_coli_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1016/j.femsle.2005.08.023 DB - PRIME DP - Unbound Medicine ER -