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RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs.
Genes Dev. 2005 Sep 15; 19(18):2176-86.GD

Abstract

Hfq-binding antisense small RNAs of Escherichia coli, SgrS and RyhB, mediate the destabilization of target mRNAs in an RNase E-dependent manner. SgrS, whose expression is induced in response to phosphosugar stress, act on the ptsG mRNA encoding a major glucose transporter, while RyhB, whose expression is induced in response to Fe depletion, acts on several mRNAs encoding Fe-binding proteins. In this report, we addressed the question of how SgrS and RyhB RNAs cooperate with RNase E to destabilize the target mRNAs. We demonstrate that Hfq along with SgrS and RyhB copurified with RNase E but not with truncated RNase E. In addition, we show that RNase E but not other degradosome components copurified with Hfq. Taken together, we conclude that RNase E forms variable ribonucleoprotein complexes with Hfq/small RNAs through its C-terminal scaffold region. These complexes, distinct from the RNA degradosome, may act as specialized RNA decay machines that initiate the degradation of mRNAs targeted by each small RNA. The present finding has uncovered the mechanical basis of mRNA destabilization mediated by bacterial small RNAs. The formation of ribonucleoprotein complexes containing RNases could be a general way by which small RNAs destabilize target mRNAs in both prokaryotes and eukaryotes.

Authors+Show Affiliations

Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16166379

Citation

Morita, Teppei, et al. "RNase E-based Ribonucleoprotein Complexes: Mechanical Basis of mRNA Destabilization Mediated By Bacterial Noncoding RNAs." Genes & Development, vol. 19, no. 18, 2005, pp. 2176-86.
Morita T, Maki K, Aiba H. RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs. Genes Dev. 2005;19(18):2176-86.
Morita, T., Maki, K., & Aiba, H. (2005). RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs. Genes & Development, 19(18), 2176-86.
Morita T, Maki K, Aiba H. RNase E-based Ribonucleoprotein Complexes: Mechanical Basis of mRNA Destabilization Mediated By Bacterial Noncoding RNAs. Genes Dev. 2005 Sep 15;19(18):2176-86. PubMed PMID: 16166379.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs. AU - Morita,Teppei, AU - Maki,Kimika, AU - Aiba,Hiroji, PY - 2005/9/17/pubmed PY - 2005/10/20/medline PY - 2005/9/17/entrez SP - 2176 EP - 86 JF - Genes & development JO - Genes Dev VL - 19 IS - 18 N2 - Hfq-binding antisense small RNAs of Escherichia coli, SgrS and RyhB, mediate the destabilization of target mRNAs in an RNase E-dependent manner. SgrS, whose expression is induced in response to phosphosugar stress, act on the ptsG mRNA encoding a major glucose transporter, while RyhB, whose expression is induced in response to Fe depletion, acts on several mRNAs encoding Fe-binding proteins. In this report, we addressed the question of how SgrS and RyhB RNAs cooperate with RNase E to destabilize the target mRNAs. We demonstrate that Hfq along with SgrS and RyhB copurified with RNase E but not with truncated RNase E. In addition, we show that RNase E but not other degradosome components copurified with Hfq. Taken together, we conclude that RNase E forms variable ribonucleoprotein complexes with Hfq/small RNAs through its C-terminal scaffold region. These complexes, distinct from the RNA degradosome, may act as specialized RNA decay machines that initiate the degradation of mRNAs targeted by each small RNA. The present finding has uncovered the mechanical basis of mRNA destabilization mediated by bacterial small RNAs. The formation of ribonucleoprotein complexes containing RNases could be a general way by which small RNAs destabilize target mRNAs in both prokaryotes and eukaryotes. SN - 0890-9369 UR - https://www.unboundmedicine.com/medline/citation/16166379/RNase_E_based_ribonucleoprotein_complexes:_mechanical_basis_of_mRNA_destabilization_mediated_by_bacterial_noncoding_RNAs_ L2 - http://www.genesdev.org/cgi/pmidlookup?view=long&pmid=16166379 DB - PRIME DP - Unbound Medicine ER -