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Rapid stereoselective separations of amphetamine derivatives with highly sulfated gamma-cyclodextrin.
Electrophoresis. 2005 Oct; 26(20):3910-20.E

Abstract

The highly sulfated gamma-CD (HS-gamma-CD) is a chiral selector widely used in CE for the enantioseparation of pharmaceutical compounds. This paper investigated different approaches to reduce the stereoselective analysis time of amphetamine (AT) derivatives according to the chiral selector concentration in the BGE. With high HS-gamma-CD concentration, tested analytes were separated in 3.5 min as anionic complexes with short-end injection technique in reversed polarity mode. However, this procedure presented some limitations in terms of efficiency and resolution, excessive Joule heating and poor compatibility with MS detection. With low HS-gamma-CD concentration, compounds were separated as cations. Conventional approaches to reduce CE analysis time demonstrated critical resolution between some analytes. Therefore, the use of the partial-filling technique compatible with MS detection was carried out. Under optimized conditions, the analysis time for the chiral separation of seven AT like compounds was reduced to 6 min. Moreover, sensitivity of CE-MS was sufficient for the determination of ATs in plasma following a simple liquid-liquid extraction.

Authors+Show Affiliations

Rudaz S
Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences, EPGL, University of Geneva, Geneva, Switzerland. Serge.Rudaz@pharm.unige.ch
Geiser L
No affiliation info available
Souverain S
No affiliation info available
Prat J
No affiliation info available
Veuthey JL
No affiliation info available

MeSH

AmphetaminesCationsElectrophoresis, CapillaryHumansStereoisomerismSulfatesTime Factorsgamma-Cyclodextrins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16167308

Citation

Rudaz, Serge, et al. "Rapid Stereoselective Separations of Amphetamine Derivatives With Highly Sulfated Gamma-cyclodextrin." Electrophoresis, vol. 26, no. 20, 2005, pp. 3910-20.
Rudaz S, Geiser L, Souverain S, et al. Rapid stereoselective separations of amphetamine derivatives with highly sulfated gamma-cyclodextrin. Electrophoresis. 2005;26(20):3910-20.
Rudaz, S., Geiser, L., Souverain, S., Prat, J., & Veuthey, J. L. (2005). Rapid stereoselective separations of amphetamine derivatives with highly sulfated gamma-cyclodextrin. Electrophoresis, 26(20), 3910-20.
Rudaz S, et al. Rapid Stereoselective Separations of Amphetamine Derivatives With Highly Sulfated Gamma-cyclodextrin. Electrophoresis. 2005;26(20):3910-20. PubMed PMID: 16167308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid stereoselective separations of amphetamine derivatives with highly sulfated gamma-cyclodextrin. AU - Rudaz,Serge, AU - Geiser,Laurent, AU - Souverain,Sandrine, AU - Prat,Josiane, AU - Veuthey,Jean-Luc, PY - 2005/9/17/pubmed PY - 2006/1/27/medline PY - 2005/9/17/entrez SP - 3910 EP - 20 JF - Electrophoresis JO - Electrophoresis VL - 26 IS - 20 N2 - The highly sulfated gamma-CD (HS-gamma-CD) is a chiral selector widely used in CE for the enantioseparation of pharmaceutical compounds. This paper investigated different approaches to reduce the stereoselective analysis time of amphetamine (AT) derivatives according to the chiral selector concentration in the BGE. With high HS-gamma-CD concentration, tested analytes were separated in 3.5 min as anionic complexes with short-end injection technique in reversed polarity mode. However, this procedure presented some limitations in terms of efficiency and resolution, excessive Joule heating and poor compatibility with MS detection. With low HS-gamma-CD concentration, compounds were separated as cations. Conventional approaches to reduce CE analysis time demonstrated critical resolution between some analytes. Therefore, the use of the partial-filling technique compatible with MS detection was carried out. Under optimized conditions, the analysis time for the chiral separation of seven AT like compounds was reduced to 6 min. Moreover, sensitivity of CE-MS was sufficient for the determination of ATs in plasma following a simple liquid-liquid extraction. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/16167308/Rapid_stereoselective_separations_of_amphetamine_derivatives_with_highly_sulfated_gamma_cyclodextrin_ DB - PRIME DP - Unbound Medicine ER -