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The Arabidopsis csb3 mutant reveals a regulatory link between salicylic acid-mediated disease resistance and the methyl-erythritol 4-phosphate pathway.
Plant J. 2005 Oct; 44(1):155-66.PJ

Abstract

We report on constitutive subtilisin3 (csb3), an Arabidopsis mutant showing strikingly enhanced resistance to biotrophic pathogens. Epistasis analyses with pad4, sid2, eds5, NahG, npr1, dth9 and cpr1 mutants revealed that the enhanced resistance of csb3 plants requires intact salicylic acid (SA) synthesis and perception. CSB3 encodes a 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase, the enzyme controlling the penultimate step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the chloroplast. CSB3 is expressed constitutively in healthy plants, and shows repression in response to bacterial infection. We also show the pharmacological complementation of the enhanced-resistance phenotype of csb3 plants with fosmidomycin, an inhibitor of the MEP pathway, and propose that CSB3 represents a point of metabolic convergence modulating the magnitude of SA-mediated disease resistance to biotrophic pathogens.

Authors+Show Affiliations

Gil MJ
Instituto de Biología Molecular y Celular de Plantas (IBMCP), UPV-CSIC, Camino de Vera, s/n, 46022 Valencia, Spain.
Coego A
No affiliation info available
Mauch-Mani B
No affiliation info available
Jordá L
No affiliation info available
Vera P
No affiliation info available

MeSH

Amino Acid SequenceArabidopsisEnzymesErythritolGene Expression Regulation, PlantMolecular Sequence DataMutationPhenotypePlant DiseasesPseudomonas syringaeSalicylic AcidSequence Homology, Amino AcidSignal TransductionSugar Phosphates

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16167903

Citation

Gil, M José, et al. "The Arabidopsis Csb3 Mutant Reveals a Regulatory Link Between Salicylic Acid-mediated Disease Resistance and the Methyl-erythritol 4-phosphate Pathway." The Plant Journal : for Cell and Molecular Biology, vol. 44, no. 1, 2005, pp. 155-66.
Gil MJ, Coego A, Mauch-Mani B, et al. The Arabidopsis csb3 mutant reveals a regulatory link between salicylic acid-mediated disease resistance and the methyl-erythritol 4-phosphate pathway. Plant J. 2005;44(1):155-66.
Gil, M. J., Coego, A., Mauch-Mani, B., Jordá, L., & Vera, P. (2005). The Arabidopsis csb3 mutant reveals a regulatory link between salicylic acid-mediated disease resistance and the methyl-erythritol 4-phosphate pathway. The Plant Journal : for Cell and Molecular Biology, 44(1), 155-66.
Gil MJ, et al. The Arabidopsis Csb3 Mutant Reveals a Regulatory Link Between Salicylic Acid-mediated Disease Resistance and the Methyl-erythritol 4-phosphate Pathway. Plant J. 2005;44(1):155-66. PubMed PMID: 16167903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Arabidopsis csb3 mutant reveals a regulatory link between salicylic acid-mediated disease resistance and the methyl-erythritol 4-phosphate pathway. AU - Gil,M José, AU - Coego,Alberto, AU - Mauch-Mani,Brigitte, AU - Jordá,Lucia, AU - Vera,Pablo, PY - 2005/9/20/pubmed PY - 2006/1/21/medline PY - 2005/9/20/entrez SP - 155 EP - 66 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 44 IS - 1 N2 - We report on constitutive subtilisin3 (csb3), an Arabidopsis mutant showing strikingly enhanced resistance to biotrophic pathogens. Epistasis analyses with pad4, sid2, eds5, NahG, npr1, dth9 and cpr1 mutants revealed that the enhanced resistance of csb3 plants requires intact salicylic acid (SA) synthesis and perception. CSB3 encodes a 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase, the enzyme controlling the penultimate step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the chloroplast. CSB3 is expressed constitutively in healthy plants, and shows repression in response to bacterial infection. We also show the pharmacological complementation of the enhanced-resistance phenotype of csb3 plants with fosmidomycin, an inhibitor of the MEP pathway, and propose that CSB3 represents a point of metabolic convergence modulating the magnitude of SA-mediated disease resistance to biotrophic pathogens. SN - 0960-7412 UR - https://www.unboundmedicine.com/medline/citation/16167903/The_Arabidopsis_csb3_mutant_reveals_a_regulatory_link_between_salicylic_acid_mediated_disease_resistance_and_the_methyl_erythritol_4_phosphate_pathway_ DB - PRIME DP - Unbound Medicine ER -