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Effect of pioglitazone on L-NAME induced hypertension in diabetic rats.
Vascul Pharmacol. 2005 Oct; 43(4):260-6.VP

Abstract

The present study investigates the effect of pioglitazone treatment on blood pressure, vascular reactivity and antioxidant enzymes in L-NAME induced hypertension in normal and STZ-diabetic rats. Diabetes was induced in male Sprague Dawley rats (200+/-15 g) by single intravenous injection of 55 mg/kg of streptozotocin (STZ). Rats were randomized into diabetic and nondiabetic groups, Nomega-nitro-L-arginine-methyl ester (L-NAME, 50 mg/kg) was administered in drinking water for 4 weeks. They were treated with pioglitazone (10 mg/kg/day, p.o.) for 4 weeks and following protocol was carried out. Blood pressure, blood glucose levels and body weight were measured. Thoracic aorta was isolated and dose response curve of phenylephrine (PE) with intact and denuded endothelium was recorded. Dose response curve of acetylcholine (Ach) and sodium nitroprusside (SNP) was recorded in precontracted rings. Lipid peroxidation, superoxide dismutase, catalase, and reduced glutathione were estimated in liver, kidney, and aorta. Pioglitazone produced no significant effect on blood glucose levels, body weight and blood pressure of L-NAME administered nondiabetic and diabetic rats. Pioglitazone treatment had no significant effect on PE induced contraction and Ach induced relaxation in L-NAME diabetic and nondiabetic rats. SNP completely relaxed aortic rings of all the groups. Higher oxidative stress in case of diabetic rats was significantly (p<0.05) reduced by pioglitazone treatment. Although pioglitazone reduced oxidative stress in diabetic rats, there was no significant effect on blood pressure as there was complete absence of nitric oxide due to administration of L-NAME. Hence from the present study it can be concluded that reduction in blood pressure in case of STZ-diabetic rats is nitric oxide mediated.

Authors+Show Affiliations

Pharmacy Department, Faculty of Technology and Engineering, M. S. University of Baroda, Kalabhavan, Baroda 390001, Gujarat, India. jayeshbm@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16168716

Citation

Majithiya, Jayesh B., et al. "Effect of Pioglitazone On L-NAME Induced Hypertension in Diabetic Rats." Vascular Pharmacology, vol. 43, no. 4, 2005, pp. 260-6.
Majithiya JB, Parmar AN, Trivedi CJ, et al. Effect of pioglitazone on L-NAME induced hypertension in diabetic rats. Vascul Pharmacol. 2005;43(4):260-6.
Majithiya, J. B., Parmar, A. N., Trivedi, C. J., & Balaraman, R. (2005). Effect of pioglitazone on L-NAME induced hypertension in diabetic rats. Vascular Pharmacology, 43(4), 260-6.
Majithiya JB, et al. Effect of Pioglitazone On L-NAME Induced Hypertension in Diabetic Rats. Vascul Pharmacol. 2005;43(4):260-6. PubMed PMID: 16168716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of pioglitazone on L-NAME induced hypertension in diabetic rats. AU - Majithiya,Jayesh B, AU - Parmar,Arvind N, AU - Trivedi,Chitrang J, AU - Balaraman,R, Y1 - 2005/09/15/ PY - 2005/06/04/received PY - 2005/08/02/revised PY - 2005/08/17/accepted PY - 2005/9/20/pubmed PY - 2006/2/24/medline PY - 2005/9/20/entrez SP - 260 EP - 6 JF - Vascular pharmacology JO - Vascul Pharmacol VL - 43 IS - 4 N2 - The present study investigates the effect of pioglitazone treatment on blood pressure, vascular reactivity and antioxidant enzymes in L-NAME induced hypertension in normal and STZ-diabetic rats. Diabetes was induced in male Sprague Dawley rats (200+/-15 g) by single intravenous injection of 55 mg/kg of streptozotocin (STZ). Rats were randomized into diabetic and nondiabetic groups, Nomega-nitro-L-arginine-methyl ester (L-NAME, 50 mg/kg) was administered in drinking water for 4 weeks. They were treated with pioglitazone (10 mg/kg/day, p.o.) for 4 weeks and following protocol was carried out. Blood pressure, blood glucose levels and body weight were measured. Thoracic aorta was isolated and dose response curve of phenylephrine (PE) with intact and denuded endothelium was recorded. Dose response curve of acetylcholine (Ach) and sodium nitroprusside (SNP) was recorded in precontracted rings. Lipid peroxidation, superoxide dismutase, catalase, and reduced glutathione were estimated in liver, kidney, and aorta. Pioglitazone produced no significant effect on blood glucose levels, body weight and blood pressure of L-NAME administered nondiabetic and diabetic rats. Pioglitazone treatment had no significant effect on PE induced contraction and Ach induced relaxation in L-NAME diabetic and nondiabetic rats. SNP completely relaxed aortic rings of all the groups. Higher oxidative stress in case of diabetic rats was significantly (p<0.05) reduced by pioglitazone treatment. Although pioglitazone reduced oxidative stress in diabetic rats, there was no significant effect on blood pressure as there was complete absence of nitric oxide due to administration of L-NAME. Hence from the present study it can be concluded that reduction in blood pressure in case of STZ-diabetic rats is nitric oxide mediated. SN - 1537-1891 UR - https://www.unboundmedicine.com/medline/citation/16168716/Effect_of_pioglitazone_on_L_NAME_induced_hypertension_in_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1537-1891(05)00175-8 DB - PRIME DP - Unbound Medicine ER -