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Selective photo-reduction of N-nitroamines combined with micellar electrokinetic chromatography and laser fluorimetric detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Nov 05; 826(1-2):160-8.JC

Abstract

N-nitroso compounds (NOC) are potent carcinogens. Reliable methods for the analysis of volatile carcinogenic NOC are well established; however selective and sensitive methods for routine analysis of thermally unstable, ionic or non-volatile NOC are still needed. For this purpose, a method based on micellar electrokinetic chromatography (MEKC) with laser induced fluorescence (LIF) detection is described for the simultaneous determination of a broad range of N-nitroso compounds. In this procedure, the nitroso group is photolytically cleaved from the NOC to yield the corresponding amine. The amines are then derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl), identified and quantified using MEKC-LIF. For the standard mixture of NOC, this method has good sensitivity and a large dynamic range. The detection limit provided by the method is 9 ppb for N-nitrosopyrrolidine.

Authors+Show Affiliations

Bell LM
Hewlett Packard Company, 1000 NE Circle Blvd., Corvallis, OR 97330, USA. leann.bell@hp.com
Murray GM
No affiliation info available

MeSH

4-Chloro-7-nitrobenzofurazanAminesCarcinogensChromatography, Micellar Electrokinetic CapillaryFluorometryLasersNitroso CompoundsOxidation-ReductionPhotolysisPyrrolidinesSensitivity and SpecificityUltraviolet Rays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16169292

Citation

Bell, LeAnn M., and George M. Murray. "Selective Photo-reduction of N-nitroamines Combined With Micellar Electrokinetic Chromatography and Laser Fluorimetric Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 826, no. 1-2, 2005, pp. 160-8.
Bell LM, Murray GM. Selective photo-reduction of N-nitroamines combined with micellar electrokinetic chromatography and laser fluorimetric detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2005;826(1-2):160-8.
Bell, L. M., & Murray, G. M. (2005). Selective photo-reduction of N-nitroamines combined with micellar electrokinetic chromatography and laser fluorimetric detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 826(1-2), 160-8.
Bell LM, Murray GM. Selective Photo-reduction of N-nitroamines Combined With Micellar Electrokinetic Chromatography and Laser Fluorimetric Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Nov 5;826(1-2):160-8. PubMed PMID: 16169292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective photo-reduction of N-nitroamines combined with micellar electrokinetic chromatography and laser fluorimetric detection. AU - Bell,LeAnn M, AU - Murray,George M, Y1 - 2005/10/05/ PY - 2005/05/23/received PY - 2005/08/11/revised PY - 2005/08/18/accepted PY - 2005/9/20/pubmed PY - 2005/12/20/medline PY - 2005/9/20/entrez SP - 160 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 826 IS - 1-2 N2 - N-nitroso compounds (NOC) are potent carcinogens. Reliable methods for the analysis of volatile carcinogenic NOC are well established; however selective and sensitive methods for routine analysis of thermally unstable, ionic or non-volatile NOC are still needed. For this purpose, a method based on micellar electrokinetic chromatography (MEKC) with laser induced fluorescence (LIF) detection is described for the simultaneous determination of a broad range of N-nitroso compounds. In this procedure, the nitroso group is photolytically cleaved from the NOC to yield the corresponding amine. The amines are then derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl), identified and quantified using MEKC-LIF. For the standard mixture of NOC, this method has good sensitivity and a large dynamic range. The detection limit provided by the method is 9 ppb for N-nitrosopyrrolidine. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16169292/Selective_photo_reduction_of_N_nitroamines_combined_with_micellar_electrokinetic_chromatography_and_laser_fluorimetric_detection_ DB - PRIME DP - Unbound Medicine ER -

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