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Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats.
Proc Natl Acad Sci U S A. 2005 Sep 27; 102(39):14040-5.PN

Abstract

Although the finding of severe acute respiratory syndrome coronavirus (SARS-CoV) in caged palm civets from live animal markets in China has provided evidence for interspecies transmission in the genesis of the SARS epidemic, subsequent studies suggested that the civet may have served only as an amplification host for SARS-CoV. In a surveillance study for CoV in noncaged animals from the wild areas of the Hong Kong Special Administration Region, we identified a CoV closely related to SARS-CoV (bat-SARS-CoV) from 23 (39%) of 59 anal swabs of wild Chinese horseshoe bats (Rhinolophus sinicus) by using RT-PCR. Sequencing and analysis of three bat-SARS-CoV genomes from samples collected at different dates showed that bat-SARS-CoV is closely related to SARS-CoV from humans and civets. Phylogenetic analysis showed that bat-SARS-CoV formed a distinct cluster with SARS-CoV as group 2b CoV, distantly related to known group 2 CoV. Most differences between the bat-SARS-CoV and SARS-CoV genomes were observed in the spike genes, ORF 3 and ORF 8, which are the regions where most variations also were observed between human and civet SARS-CoV genomes. In addition, the presence of a 29-bp insertion in ORF 8 of bat-SARS-CoV genome, not in most human SARS-CoV genomes, suggests that it has a common ancestor with civet SARS-CoV. Antibody against recombinant bat-SARS-CoV nucleocapsid protein was detected in 84% of Chinese horseshoe bats by using an enzyme immunoassay. Neutralizing antibody to human SARS-CoV also was detected in bats with lower viral loads. Precautions should be exercised in the handling of these animals.

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Authors+Show Affiliations

Lau SK
Department of Microbiology, Research Centre of Infection and Immunology, State Key Laboratory of Emerging Infectious Diseases, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China.
Woo PC
No affiliation info available
Li KS
No affiliation info available
Huang Y
No affiliation info available
Tsoi HW
No affiliation info available
Wong BH
No affiliation info available
Wong SS
No affiliation info available
Leung SY
No affiliation info available
Chan KH
No affiliation info available
Yuen KY
No affiliation info available

MeSH

Amino Acid SequenceAnimalsBase SequenceChiropteraGenome, ViralHong KongMolecular Sequence DataPhylogenySevere acute respiratory syndrome-related coronavirusSequence Analysis, DNAViral Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16169905

Clinical Trial Links

Primary Prevention of Infection by COVID-19 in Workers

Citation

Lau, Susanna K P., et al. "Severe Acute Respiratory Syndrome Coronavirus-like Virus in Chinese Horseshoe Bats." Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 39, 2005, pp. 14040-5.
Lau SK, Woo PC, Li KS, et al. Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats. Proc Natl Acad Sci U S A. 2005;102(39):14040-5.
Lau, S. K., Woo, P. C., Li, K. S., Huang, Y., Tsoi, H. W., Wong, B. H., Wong, S. S., Leung, S. Y., Chan, K. H., & Yuen, K. Y. (2005). Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats. Proceedings of the National Academy of Sciences of the United States of America, 102(39), 14040-5.
Lau SK, et al. Severe Acute Respiratory Syndrome Coronavirus-like Virus in Chinese Horseshoe Bats. Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14040-5. PubMed PMID: 16169905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats. AU - Lau,Susanna K P, AU - Woo,Patrick C Y, AU - Li,Kenneth S M, AU - Huang,Yi, AU - Tsoi,Hoi-Wah, AU - Wong,Beatrice H L, AU - Wong,Samson S Y, AU - Leung,Suet-Yi, AU - Chan,Kwok-Hung, AU - Yuen,Kwok-Yung, Y1 - 2005/09/16/ PY - 2005/9/20/pubmed PY - 2005/12/13/medline PY - 2005/9/20/entrez SP - 14040 EP - 5 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 102 IS - 39 N2 - Although the finding of severe acute respiratory syndrome coronavirus (SARS-CoV) in caged palm civets from live animal markets in China has provided evidence for interspecies transmission in the genesis of the SARS epidemic, subsequent studies suggested that the civet may have served only as an amplification host for SARS-CoV. In a surveillance study for CoV in noncaged animals from the wild areas of the Hong Kong Special Administration Region, we identified a CoV closely related to SARS-CoV (bat-SARS-CoV) from 23 (39%) of 59 anal swabs of wild Chinese horseshoe bats (Rhinolophus sinicus) by using RT-PCR. Sequencing and analysis of three bat-SARS-CoV genomes from samples collected at different dates showed that bat-SARS-CoV is closely related to SARS-CoV from humans and civets. Phylogenetic analysis showed that bat-SARS-CoV formed a distinct cluster with SARS-CoV as group 2b CoV, distantly related to known group 2 CoV. Most differences between the bat-SARS-CoV and SARS-CoV genomes were observed in the spike genes, ORF 3 and ORF 8, which are the regions where most variations also were observed between human and civet SARS-CoV genomes. In addition, the presence of a 29-bp insertion in ORF 8 of bat-SARS-CoV genome, not in most human SARS-CoV genomes, suggests that it has a common ancestor with civet SARS-CoV. Antibody against recombinant bat-SARS-CoV nucleocapsid protein was detected in 84% of Chinese horseshoe bats by using an enzyme immunoassay. Neutralizing antibody to human SARS-CoV also was detected in bats with lower viral loads. Precautions should be exercised in the handling of these animals. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/16169905/Severe_acute_respiratory_syndrome_coronavirus_like_virus_in_Chinese_horseshoe_bats_ DB - PRIME DP - Unbound Medicine ER -