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Blastomyces dermatitidis antigen detection in urine specimens from dogs with blastomycosis using a competitive binding inhibition ELISA.
Mycopathologia. 2005 Sep; 160(2):137-42.M

Abstract

A competitive binding inhibition enzyme linked immunosorbent assay (ELISA) was used to detect Blastomyces dermatitidis antigens in urine specimens from dogs with blastomycosis. Sera from rabbits immunized with B. dermatitidis killed whole yeast cells were used as the primary antibody in the competitive ELISA. This initial study was performed to determine if B. dermatitidis antigen detection was possible and to test the efficacy of the rabbit sera as a primary antibody. An indirect ELISA was also performed to compare antigen detection in urine to antibody detection in the sera of the infected dogs. The results indicate 100% (36/36 specimens) detection of both antigen and antibody. Cross reactivity with Histoplasma capsulatum, as well as non-specific binding with the normal urine specimens, was observed with the competitive binding inhibition ELISA.

Authors+Show Affiliations

Department of Biological Sciences, Idaho State University, Pocatello, 83209, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16170609

Citation

Shurley, J F., et al. "Blastomyces Dermatitidis Antigen Detection in Urine Specimens From Dogs With Blastomycosis Using a Competitive Binding Inhibition ELISA." Mycopathologia, vol. 160, no. 2, 2005, pp. 137-42.
Shurley JF, Legendre AM, Scalarone GM. Blastomyces dermatitidis antigen detection in urine specimens from dogs with blastomycosis using a competitive binding inhibition ELISA. Mycopathologia. 2005;160(2):137-42.
Shurley, J. F., Legendre, A. M., & Scalarone, G. M. (2005). Blastomyces dermatitidis antigen detection in urine specimens from dogs with blastomycosis using a competitive binding inhibition ELISA. Mycopathologia, 160(2), 137-42.
Shurley JF, Legendre AM, Scalarone GM. Blastomyces Dermatitidis Antigen Detection in Urine Specimens From Dogs With Blastomycosis Using a Competitive Binding Inhibition ELISA. Mycopathologia. 2005;160(2):137-42. PubMed PMID: 16170609.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Blastomyces dermatitidis antigen detection in urine specimens from dogs with blastomycosis using a competitive binding inhibition ELISA. AU - Shurley,J F, AU - Legendre,A M, AU - Scalarone,G M, PY - 2004/04/15/received PY - 2005/06/06/accepted PY - 2005/9/20/pubmed PY - 2006/2/4/medline PY - 2005/9/20/entrez SP - 137 EP - 42 JF - Mycopathologia JO - Mycopathologia VL - 160 IS - 2 N2 - A competitive binding inhibition enzyme linked immunosorbent assay (ELISA) was used to detect Blastomyces dermatitidis antigens in urine specimens from dogs with blastomycosis. Sera from rabbits immunized with B. dermatitidis killed whole yeast cells were used as the primary antibody in the competitive ELISA. This initial study was performed to determine if B. dermatitidis antigen detection was possible and to test the efficacy of the rabbit sera as a primary antibody. An indirect ELISA was also performed to compare antigen detection in urine to antibody detection in the sera of the infected dogs. The results indicate 100% (36/36 specimens) detection of both antigen and antibody. Cross reactivity with Histoplasma capsulatum, as well as non-specific binding with the normal urine specimens, was observed with the competitive binding inhibition ELISA. SN - 0301-486X UR - https://www.unboundmedicine.com/medline/citation/16170609/Blastomyces_dermatitidis_antigen_detection_in_urine_specimens_from_dogs_with_blastomycosis_using_a_competitive_binding_inhibition_ELISA_ L2 - https://doi.org/10.1007/s11046-005-3153-9 DB - PRIME DP - Unbound Medicine ER -