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Free CoA-mediated regulation of intermediary and central metabolism: an hypothesis which accounts for the excretion of alpha-ketoglutarate during aerobic growth of Escherichia coli on acetate.
Res Microbiol. 2005 Sep; 156(8):874-9.RM

Abstract

During growth of Escherichia coli on acetate, phosphotransacetylase and alpha-ketoglutarate dehydrogenase are in direct competition for their common co-factor, HS-CoA. Such competition is resolved in favour of phosphotransacetylase, thus rendering alpha-ketoglutarate dehydrogenase rate-limiting (controlling) and, in turn, creating a bottleneck at the level of alpha-ketoglutarate in the Krebs cycle. Accumulation of alpha-ketoglutarate is then balanced by its excretion. Addition of pyruvate, glucose or any glycolytic intermediate to acetate-grown culture relieves such a bottleneck by reversing carbon flow through phosphotransacetylase to give acetyl phosphate and much-needed HS-CoA. The urgent need for HS-CoA by the primordial organism might therefore have provided the selective pressure that led to the co-evolution of phosphotransacetylase and the two-malate synthase isoenzymes.

Authors+Show Affiliations

School of Life Sciences, Faculty of Health and Life Sciences, Napier University, Edinburgh, EH10 5DT, Scotland, UK.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16171983

Citation

El-Mansi, Mansi. "Free CoA-mediated Regulation of Intermediary and Central Metabolism: an Hypothesis Which Accounts for the Excretion of Alpha-ketoglutarate During Aerobic Growth of Escherichia Coli On Acetate." Research in Microbiology, vol. 156, no. 8, 2005, pp. 874-9.
El-Mansi M. Free CoA-mediated regulation of intermediary and central metabolism: an hypothesis which accounts for the excretion of alpha-ketoglutarate during aerobic growth of Escherichia coli on acetate. Res Microbiol. 2005;156(8):874-9.
El-Mansi, M. (2005). Free CoA-mediated regulation of intermediary and central metabolism: an hypothesis which accounts for the excretion of alpha-ketoglutarate during aerobic growth of Escherichia coli on acetate. Research in Microbiology, 156(8), 874-9.
El-Mansi M. Free CoA-mediated Regulation of Intermediary and Central Metabolism: an Hypothesis Which Accounts for the Excretion of Alpha-ketoglutarate During Aerobic Growth of Escherichia Coli On Acetate. Res Microbiol. 2005;156(8):874-9. PubMed PMID: 16171983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Free CoA-mediated regulation of intermediary and central metabolism: an hypothesis which accounts for the excretion of alpha-ketoglutarate during aerobic growth of Escherichia coli on acetate. A1 - El-Mansi,Mansi, Y1 - 2005/06/16/ PY - 2005/01/11/received PY - 2005/04/12/revised PY - 2005/04/14/accepted PY - 2005/9/21/pubmed PY - 2006/1/18/medline PY - 2005/9/21/entrez SP - 874 EP - 9 JF - Research in microbiology JO - Res Microbiol VL - 156 IS - 8 N2 - During growth of Escherichia coli on acetate, phosphotransacetylase and alpha-ketoglutarate dehydrogenase are in direct competition for their common co-factor, HS-CoA. Such competition is resolved in favour of phosphotransacetylase, thus rendering alpha-ketoglutarate dehydrogenase rate-limiting (controlling) and, in turn, creating a bottleneck at the level of alpha-ketoglutarate in the Krebs cycle. Accumulation of alpha-ketoglutarate is then balanced by its excretion. Addition of pyruvate, glucose or any glycolytic intermediate to acetate-grown culture relieves such a bottleneck by reversing carbon flow through phosphotransacetylase to give acetyl phosphate and much-needed HS-CoA. The urgent need for HS-CoA by the primordial organism might therefore have provided the selective pressure that led to the co-evolution of phosphotransacetylase and the two-malate synthase isoenzymes. SN - 0923-2508 UR - https://www.unboundmedicine.com/medline/citation/16171983/Free_CoA_mediated_regulation_of_intermediary_and_central_metabolism:_an_hypothesis_which_accounts_for_the_excretion_of_alpha_ketoglutarate_during_aerobic_growth_of_Escherichia_coli_on_acetate_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-2508(05)00117-8 DB - PRIME DP - Unbound Medicine ER -