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[Continuous ethanol fermentation using self-flocculating yeast in multi-stage suspended bioreactors coupled with directly recycling of waste distillage].
Sheng Wu Gong Cheng Xue Bao. 2005 Jul; 21(4):628-32.SW

Abstract

A fermentation system composed of four airlift suspended-bed bioreactors in series and with a total working volume of 4800 mL was established. Continuous ethanol fermentation using self-flocculating yeast SPSC01, a fusant from Saccharomyces cerevisiae and Schizosaccharomyces pombe, and two-stage enzymatic hydrolyte of dry milling corn powder, was continuously run for 120 days. All of the backset distillage collected after distilling the final beer was used to mix the corn powder and no any other wastes except the solid residue of corn powder was discharged from the fermentation system, which guaranteed the distillage to be recycled at its maximum. The experimental results revealed that both ethanol and residual sugar in the final beer could be maintained relatively stable with their average levels of 93.6 and 7.9 g/L, respectively when the fermentation system was operated at the dilution rate of 0.05 h(-1). Parameter oscillations reported previously were also observed for the first and second bioreactors, but were effectively attenuated thereafter, which indicated that high yeast cell concentrations resulted from the self-immobilization of this special self-flocculating strain contributed to damp these oscillations. The monitoring of residual nitrogen and phosphor indicated that the accumulations of these nutritional elements occurred and the amount of these inorganic salts supplemented in the substrate should be decreased properly.

Authors+Show Affiliations

Department of Bioscience and Bioengineering, Dalian university of Technology, Dalian 116023, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16176104

Citation

Yan, Zheng, et al. "[Continuous Ethanol Fermentation Using Self-flocculating Yeast in Multi-stage Suspended Bioreactors Coupled With Directly Recycling of Waste Distillage]." Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, vol. 21, no. 4, 2005, pp. 628-32.
Yan Z, Zi LH, Li N, et al. [Continuous ethanol fermentation using self-flocculating yeast in multi-stage suspended bioreactors coupled with directly recycling of waste distillage]. Sheng Wu Gong Cheng Xue Bao. 2005;21(4):628-32.
Yan, Z., Zi, L. H., Li, N., Wang, F., & Bai, F. W. (2005). [Continuous ethanol fermentation using self-flocculating yeast in multi-stage suspended bioreactors coupled with directly recycling of waste distillage]. Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, 21(4), 628-32.
Yan Z, et al. [Continuous Ethanol Fermentation Using Self-flocculating Yeast in Multi-stage Suspended Bioreactors Coupled With Directly Recycling of Waste Distillage]. Sheng Wu Gong Cheng Xue Bao. 2005;21(4):628-32. PubMed PMID: 16176104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Continuous ethanol fermentation using self-flocculating yeast in multi-stage suspended bioreactors coupled with directly recycling of waste distillage]. AU - Yan,Zheng, AU - Zi,Li-Han, AU - Li,Ning, AU - Wang,Fang, AU - Bai,Feng-Wu, PY - 2005/9/24/pubmed PY - 2009/7/23/medline PY - 2005/9/24/entrez SP - 628 EP - 32 JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology JO - Sheng Wu Gong Cheng Xue Bao VL - 21 IS - 4 N2 - A fermentation system composed of four airlift suspended-bed bioreactors in series and with a total working volume of 4800 mL was established. Continuous ethanol fermentation using self-flocculating yeast SPSC01, a fusant from Saccharomyces cerevisiae and Schizosaccharomyces pombe, and two-stage enzymatic hydrolyte of dry milling corn powder, was continuously run for 120 days. All of the backset distillage collected after distilling the final beer was used to mix the corn powder and no any other wastes except the solid residue of corn powder was discharged from the fermentation system, which guaranteed the distillage to be recycled at its maximum. The experimental results revealed that both ethanol and residual sugar in the final beer could be maintained relatively stable with their average levels of 93.6 and 7.9 g/L, respectively when the fermentation system was operated at the dilution rate of 0.05 h(-1). Parameter oscillations reported previously were also observed for the first and second bioreactors, but were effectively attenuated thereafter, which indicated that high yeast cell concentrations resulted from the self-immobilization of this special self-flocculating strain contributed to damp these oscillations. The monitoring of residual nitrogen and phosphor indicated that the accumulations of these nutritional elements occurred and the amount of these inorganic salts supplemented in the substrate should be decreased properly. SN - 1000-3061 UR - https://www.unboundmedicine.com/medline/citation/16176104/[Continuous_ethanol_fermentation_using_self_flocculating_yeast_in_multi_stage_suspended_bioreactors_coupled_with_directly_recycling_of_waste_distillage]_ DB - PRIME DP - Unbound Medicine ER -