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Saccharomyces cerevisiae adaptation to weak acids involves the transcription factor Haa1p and Haa1p-regulated genes.
Biochem Biophys Res Commun. 2005 Nov 11; 337(1):95-103.BB

Abstract

The understanding of the molecular mechanisms that may contribute to counteract the deleterious effects of organic acids as fungistatic agents is essential to guide suitable preservation strategies. In this work, we show that the recently identified transcription factor Haa1p is required for a more rapid adaptation of a yeast cell population to several weak acid food preservatives. Maximal protection is exerted against the short-chain length acetic or propionic acids. The transcription of nine Haa1p-target genes, many of which are predicted to encode membrane proteins of unknown or poorly characterized function, is activated under weak acid stress. The Haa1-regulated genes required for a more rapid yeast adaptation to weak acids include TPO2 and TPO3, encoding two predicted plasma membrane multidrug transporters of the major facilitator superfamily, and YGP1, encoding a poorly characterized cell wall glycoprotein. The acetic acid-induced prolongation of the lag phase of unadapted cell populations lacking HAA1 or TPO3, compared with wild-type population, was correlated with the level of the acid accumulated into the stressed cells.

Authors+Show Affiliations

Biological Sciences Research Group, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, 1049-001 Lisboa, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16176797

Citation

Fernandes, A R., et al. "Saccharomyces Cerevisiae Adaptation to Weak Acids Involves the Transcription Factor Haa1p and Haa1p-regulated Genes." Biochemical and Biophysical Research Communications, vol. 337, no. 1, 2005, pp. 95-103.
Fernandes AR, Mira NP, Vargas RC, et al. Saccharomyces cerevisiae adaptation to weak acids involves the transcription factor Haa1p and Haa1p-regulated genes. Biochem Biophys Res Commun. 2005;337(1):95-103.
Fernandes, A. R., Mira, N. P., Vargas, R. C., Canelhas, I., & Sá-Correia, I. (2005). Saccharomyces cerevisiae adaptation to weak acids involves the transcription factor Haa1p and Haa1p-regulated genes. Biochemical and Biophysical Research Communications, 337(1), 95-103.
Fernandes AR, et al. Saccharomyces Cerevisiae Adaptation to Weak Acids Involves the Transcription Factor Haa1p and Haa1p-regulated Genes. Biochem Biophys Res Commun. 2005 Nov 11;337(1):95-103. PubMed PMID: 16176797.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Saccharomyces cerevisiae adaptation to weak acids involves the transcription factor Haa1p and Haa1p-regulated genes. AU - Fernandes,A R, AU - Mira,N P, AU - Vargas,R C, AU - Canelhas,I, AU - Sá-Correia,I, PY - 2005/09/02/received PY - 2005/09/05/accepted PY - 2005/9/24/pubmed PY - 2005/11/15/medline PY - 2005/9/24/entrez SP - 95 EP - 103 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 337 IS - 1 N2 - The understanding of the molecular mechanisms that may contribute to counteract the deleterious effects of organic acids as fungistatic agents is essential to guide suitable preservation strategies. In this work, we show that the recently identified transcription factor Haa1p is required for a more rapid adaptation of a yeast cell population to several weak acid food preservatives. Maximal protection is exerted against the short-chain length acetic or propionic acids. The transcription of nine Haa1p-target genes, many of which are predicted to encode membrane proteins of unknown or poorly characterized function, is activated under weak acid stress. The Haa1-regulated genes required for a more rapid yeast adaptation to weak acids include TPO2 and TPO3, encoding two predicted plasma membrane multidrug transporters of the major facilitator superfamily, and YGP1, encoding a poorly characterized cell wall glycoprotein. The acetic acid-induced prolongation of the lag phase of unadapted cell populations lacking HAA1 or TPO3, compared with wild-type population, was correlated with the level of the acid accumulated into the stressed cells. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/16176797/Saccharomyces_cerevisiae_adaptation_to_weak_acids_involves_the_transcription_factor_Haa1p_and_Haa1p_regulated_genes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(05)02004-8 DB - PRIME DP - Unbound Medicine ER -