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BAC libraries of Triticum urartu, Aegilops speltoides and Ae. tauschii, the diploid ancestors of polyploid wheat.
Theor Appl Genet. 2005 Nov; 111(8):1617-22.TA

Abstract

Triticum urartu, Aegilops speltoides and Ae. tauschii are respectively the immediate diploid sources, or their closest relatives, of the A, B and D genomes of polyploid wheats. Here we report the construction and characterization of arrayed large-insert libraries in a bacterial artificial chromosome (BAC) vector, one for each of these diploid species. The libraries are equivalent to 3.7, 5.4 and 4.1 of the T. urartu, Ae. speltoides, Ae. tauschii genomes, respectively. The predicted levels of genome coverage were confirmed by library hybridization with single-copy genes. The libraries were used to estimate the proportion of known repeated nucleotide sequences and gene content in each genome by BAC-end sequencing. Repeated sequence families previously detected in Triticeae accounted for 57, 61 and 57% of the T. urartu, Ae. speltoides and Ae. tauschii genomes, and coding regions accounted for 5.8, 4.5 and 4.8%, respectively.

Authors+Show Affiliations

Akhunov ED
Department of Plant Sciences, University of California, Davis, CA 95616, USA.
Akhunova AR
No affiliation info available
Dvorák J
No affiliation info available

MeSH

Base SequenceChromosomes, Artificial, BacterialDNA PrimersGenome, PlantMolecular Sequence DataPolyploidySequence Analysis, DNASpecies SpecificityTriticum

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16177898

Citation

Akhunov, E D., et al. "BAC Libraries of Triticum Urartu, Aegilops Speltoides and Ae. Tauschii, the Diploid Ancestors of Polyploid Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 111, no. 8, 2005, pp. 1617-22.
Akhunov ED, Akhunova AR, Dvorák J. BAC libraries of Triticum urartu, Aegilops speltoides and Ae. tauschii, the diploid ancestors of polyploid wheat. Theor Appl Genet. 2005;111(8):1617-22.
Akhunov, E. D., Akhunova, A. R., & Dvorák, J. (2005). BAC libraries of Triticum urartu, Aegilops speltoides and Ae. tauschii, the diploid ancestors of polyploid wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 111(8), 1617-22.
Akhunov ED, Akhunova AR, Dvorák J. BAC Libraries of Triticum Urartu, Aegilops Speltoides and Ae. Tauschii, the Diploid Ancestors of Polyploid Wheat. Theor Appl Genet. 2005;111(8):1617-22. PubMed PMID: 16177898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - BAC libraries of Triticum urartu, Aegilops speltoides and Ae. tauschii, the diploid ancestors of polyploid wheat. AU - Akhunov,E D, AU - Akhunova,A R, AU - Dvorák,J, Y1 - 2005/11/10/ PY - 2005/06/14/received PY - 2005/08/22/accepted PY - 2005/9/24/pubmed PY - 2006/6/23/medline PY - 2005/9/24/entrez SP - 1617 EP - 22 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 111 IS - 8 N2 - Triticum urartu, Aegilops speltoides and Ae. tauschii are respectively the immediate diploid sources, or their closest relatives, of the A, B and D genomes of polyploid wheats. Here we report the construction and characterization of arrayed large-insert libraries in a bacterial artificial chromosome (BAC) vector, one for each of these diploid species. The libraries are equivalent to 3.7, 5.4 and 4.1 of the T. urartu, Ae. speltoides, Ae. tauschii genomes, respectively. The predicted levels of genome coverage were confirmed by library hybridization with single-copy genes. The libraries were used to estimate the proportion of known repeated nucleotide sequences and gene content in each genome by BAC-end sequencing. Repeated sequence families previously detected in Triticeae accounted for 57, 61 and 57% of the T. urartu, Ae. speltoides and Ae. tauschii genomes, and coding regions accounted for 5.8, 4.5 and 4.8%, respectively. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/16177898/BAC_libraries_of_Triticum_urartu_Aegilops_speltoides_and_Ae__tauschii_the_diploid_ancestors_of_polyploid_wheat_ DB - PRIME DP - Unbound Medicine ER -