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Effect of phase limited inhibition of MyoD expression on the terminal differentiation of bovine myoblasts: no alteration of Myf5 or myogenin expression.
Dev Growth Differ. 2005 Sep; 47(7):483-92.DG

Abstract

To investigate the roles played by MyoD in the terminal differentiation of satellite cell-derived myoblasts, the effect of antisense inhibition of MyoD expression was examined in bovine adult myoblast culture, in which inhibition treatment was limited to the terminal differentiation phase. MyoD antisense oligonucleotide DNA (AS-mD) suppressed the formation of multinucleated myotubes in the cell culture. Myotube formation was suppressed even when AS-mD treatment was limited to the period preceding the onset of myotube formation. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed that treatment with AS-mD suppressed the expression of myosin heavy chain embryonic isoform and troponin T isoforms at 4 days after the induction of differentiation. AS-mD also suppressed the expression of MRF4, but did not alter the expression of either Myf5 or myogenin, in contrast to previous results using mouse cells possessing MyoD(-/-) genetic background. These findings suggest that MyoD controls myogenesis but not Myf5 or myogenin mRNA expression during the terminal differentiation phase. Furthermore, among the alpha4, alpha5, alpha6, and alpha7 integrins, alpha4, alpha5, and alpha7 integrin expression was suppressed by AS-mD treatment, in parallel with the suppression of myotube formation, which suggests that MyoD controls myotube formation by regulating the expression of alpha4, alpha5, and alpha7 integrins.

Authors+Show Affiliations

Department of Animal Products, National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki 305-0901, Japan. muros@affrc.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16179075

Citation

Muroya, Susumu, et al. "Effect of Phase Limited Inhibition of MyoD Expression On the Terminal Differentiation of Bovine Myoblasts: No Alteration of Myf5 or Myogenin Expression." Development, Growth & Differentiation, vol. 47, no. 7, 2005, pp. 483-92.
Muroya S, Nakajima I, Oe M, et al. Effect of phase limited inhibition of MyoD expression on the terminal differentiation of bovine myoblasts: no alteration of Myf5 or myogenin expression. Dev Growth Differ. 2005;47(7):483-92.
Muroya, S., Nakajima, I., Oe, M., & Chikuni, K. (2005). Effect of phase limited inhibition of MyoD expression on the terminal differentiation of bovine myoblasts: no alteration of Myf5 or myogenin expression. Development, Growth & Differentiation, 47(7), 483-92.
Muroya S, et al. Effect of Phase Limited Inhibition of MyoD Expression On the Terminal Differentiation of Bovine Myoblasts: No Alteration of Myf5 or Myogenin Expression. Dev Growth Differ. 2005;47(7):483-92. PubMed PMID: 16179075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of phase limited inhibition of MyoD expression on the terminal differentiation of bovine myoblasts: no alteration of Myf5 or myogenin expression. AU - Muroya,Susumu, AU - Nakajima,Ikuyo, AU - Oe,Mika, AU - Chikuni,Koichi, PY - 2005/9/24/pubmed PY - 2006/1/27/medline PY - 2005/9/24/entrez SP - 483 EP - 92 JF - Development, growth & differentiation JO - Dev Growth Differ VL - 47 IS - 7 N2 - To investigate the roles played by MyoD in the terminal differentiation of satellite cell-derived myoblasts, the effect of antisense inhibition of MyoD expression was examined in bovine adult myoblast culture, in which inhibition treatment was limited to the terminal differentiation phase. MyoD antisense oligonucleotide DNA (AS-mD) suppressed the formation of multinucleated myotubes in the cell culture. Myotube formation was suppressed even when AS-mD treatment was limited to the period preceding the onset of myotube formation. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed that treatment with AS-mD suppressed the expression of myosin heavy chain embryonic isoform and troponin T isoforms at 4 days after the induction of differentiation. AS-mD also suppressed the expression of MRF4, but did not alter the expression of either Myf5 or myogenin, in contrast to previous results using mouse cells possessing MyoD(-/-) genetic background. These findings suggest that MyoD controls myogenesis but not Myf5 or myogenin mRNA expression during the terminal differentiation phase. Furthermore, among the alpha4, alpha5, alpha6, and alpha7 integrins, alpha4, alpha5, and alpha7 integrin expression was suppressed by AS-mD treatment, in parallel with the suppression of myotube formation, which suggests that MyoD controls myotube formation by regulating the expression of alpha4, alpha5, and alpha7 integrins. SN - 0012-1592 UR - https://www.unboundmedicine.com/medline/citation/16179075/Effect_of_phase_limited_inhibition_of_MyoD_expression_on_the_terminal_differentiation_of_bovine_myoblasts:_no_alteration_of_Myf5_or_myogenin_expression_ L2 - https://doi.org/10.1111/j.1440-169X.2005.00822.x DB - PRIME DP - Unbound Medicine ER -