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Polypyrimidine tract-binding protein interacts with the 3' stem-loop region of Japanese encephalitis virus negative-strand RNA.
Virus Res. 2006 Feb; 115(2):131-40.VR

Abstract

The 3' stem-loop (SL) region of positive- and negative-strand RNA of Japanese encephalitis virus (JEV), like that of other flaviviruses, may function as cis-acting signals during RNA replication. In order to demonstrate the specific interaction between JEV 3' SL regions and BHK-21 cellular proteins, we performed gel mobility shift assay and UV-induced cross-linking assay. We identified seven cellular proteins of 110, 87, 67, 45, 38, 34, and 30 kDa that bound to the (+)3' SL RNA, and eight cellular proteins of 138, 110, 87, 67, 55, 52, 38, and 34 kDa that bound to the (-)3' SL RNA. The 55 kDa protein was identified as the polypyrimidine tract-binding (PTB) protein by immunoprecipitation assay. These data suggest that the 3' SL regions of JEV-RNA of both polarities may act as recruiting signals for the components of viral replication complexes including host cell-derived PTB protein.

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Authors+Show Affiliations

Kim SM
Department of Biology, College of Sciences, Kyung Hee University, Dongdaemun-gu, Seoul 130-701, South Korea.
Jeong YS
No affiliation info available

MeSH

3' Untranslated RegionsAnimalsCell LineCricetinaeElectrophoretic Mobility Shift AssayEncephalitis Virus, JapaneseImmunoprecipitationMolecular WeightNucleic Acid ConformationPolypyrimidine Tract-Binding ProteinProtein BindingProteinsRNA, Viral

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16181699

Citation

Kim, Seong Man, and Yong Seok Jeong. "Polypyrimidine Tract-binding Protein Interacts With the 3' Stem-loop Region of Japanese Encephalitis Virus Negative-strand RNA." Virus Research, vol. 115, no. 2, 2006, pp. 131-40.
Kim SM, Jeong YS. Polypyrimidine tract-binding protein interacts with the 3' stem-loop region of Japanese encephalitis virus negative-strand RNA. Virus Res. 2006;115(2):131-40.
Kim, S. M., & Jeong, Y. S. (2006). Polypyrimidine tract-binding protein interacts with the 3' stem-loop region of Japanese encephalitis virus negative-strand RNA. Virus Research, 115(2), 131-40.
Kim SM, Jeong YS. Polypyrimidine Tract-binding Protein Interacts With the 3' Stem-loop Region of Japanese Encephalitis Virus Negative-strand RNA. Virus Res. 2006;115(2):131-40. PubMed PMID: 16181699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polypyrimidine tract-binding protein interacts with the 3' stem-loop region of Japanese encephalitis virus negative-strand RNA. AU - Kim,Seong Man, AU - Jeong,Yong Seok, Y1 - 2005/09/21/ PY - 2005/05/02/received PY - 2005/07/18/revised PY - 2005/07/18/accepted PY - 2005/9/27/pubmed PY - 2006/5/17/medline PY - 2005/9/27/entrez SP - 131 EP - 40 JF - Virus research JO - Virus Res VL - 115 IS - 2 N2 - The 3' stem-loop (SL) region of positive- and negative-strand RNA of Japanese encephalitis virus (JEV), like that of other flaviviruses, may function as cis-acting signals during RNA replication. In order to demonstrate the specific interaction between JEV 3' SL regions and BHK-21 cellular proteins, we performed gel mobility shift assay and UV-induced cross-linking assay. We identified seven cellular proteins of 110, 87, 67, 45, 38, 34, and 30 kDa that bound to the (+)3' SL RNA, and eight cellular proteins of 138, 110, 87, 67, 55, 52, 38, and 34 kDa that bound to the (-)3' SL RNA. The 55 kDa protein was identified as the polypyrimidine tract-binding (PTB) protein by immunoprecipitation assay. These data suggest that the 3' SL regions of JEV-RNA of both polarities may act as recruiting signals for the components of viral replication complexes including host cell-derived PTB protein. SN - 0168-1702 UR - https://www.unboundmedicine.com/medline/citation/16181699/Polypyrimidine_tract_binding_protein_interacts_with_the_3'_stem_loop_region_of_Japanese_encephalitis_virus_negative_strand_RNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(05)00239-X DB - PRIME DP - Unbound Medicine ER -