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Validation of a method to detect cocaine and its metabolites in nails by gas chromatography-mass spectrometry.
Forensic Sci Int. 2006 Jun 02; 159(2-3):218-22.FS

Abstract

The objective of the present work was to compare previously published methods and provide validation data to detect simultaneously cocaine (COC), benzoylecgonine (BE) and norcocaine (NCOC) in nail. Finger and toenail samples (5mg) were cut in very small pieces and submitted to an initial procedure for external decontamination. Methanol (3 ml) was used to release analytes from the matrix. A cleanup step was performed simultaneously by solid-phase extraction (SPE) and the residue was derivatized with pentafluoropropionic anhydride/pentafluoropropanol (PFPA/PFP). Gas chromatography-mass spectrometry (GC-MS) was used to detect the analytes in selected ion monitoring mode (SIM). Confidence parameters of validation of the method were: recovery, intra- and inter-assay precision, as well as limit of detection (LOD) of the analytes. The limits of detection were: 3.5 ng/mg for NCOC and 3.0 ng/mg for COC and BE. Good intra-assay precision was observed for all detected substances (coefficient of variation (CV)<11%). The inter-assay precision for norcocaine and benzoylecgonine were <4%. For intra- and inter-assay precision deuterated internal standards were used. Toenail and fingernail samples from eight declared cocaine users were submitted to the validated method.

Authors+Show Affiliations

College of Pharmaceutical Sciences, Toxicology, University of São Paulo, USP, Av. Professor Lineu Prestes, 580 B13B CEP 05508-900, São Paulo, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

16183230

Citation

Valente-Campos, Simone, et al. "Validation of a Method to Detect Cocaine and Its Metabolites in Nails By Gas Chromatography-mass Spectrometry." Forensic Science International, vol. 159, no. 2-3, 2006, pp. 218-22.
Valente-Campos S, Yonamine M, de Moraes Moreau RL, et al. Validation of a method to detect cocaine and its metabolites in nails by gas chromatography-mass spectrometry. Forensic Sci Int. 2006;159(2-3):218-22.
Valente-Campos, S., Yonamine, M., de Moraes Moreau, R. L., & Silva, O. A. (2006). Validation of a method to detect cocaine and its metabolites in nails by gas chromatography-mass spectrometry. Forensic Science International, 159(2-3), 218-22.
Valente-Campos S, et al. Validation of a Method to Detect Cocaine and Its Metabolites in Nails By Gas Chromatography-mass Spectrometry. Forensic Sci Int. 2006 Jun 2;159(2-3):218-22. PubMed PMID: 16183230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validation of a method to detect cocaine and its metabolites in nails by gas chromatography-mass spectrometry. AU - Valente-Campos,Simone, AU - Yonamine,Mauricio, AU - de Moraes Moreau,Regina Lucia, AU - Silva,Ovandir Alves, Y1 - 2005/09/23/ PY - 2004/10/18/received PY - 2005/07/28/revised PY - 2005/07/28/accepted PY - 2005/9/27/pubmed PY - 2006/7/6/medline PY - 2005/9/27/entrez SP - 218 EP - 22 JF - Forensic science international JO - Forensic Sci Int VL - 159 IS - 2-3 N2 - The objective of the present work was to compare previously published methods and provide validation data to detect simultaneously cocaine (COC), benzoylecgonine (BE) and norcocaine (NCOC) in nail. Finger and toenail samples (5mg) were cut in very small pieces and submitted to an initial procedure for external decontamination. Methanol (3 ml) was used to release analytes from the matrix. A cleanup step was performed simultaneously by solid-phase extraction (SPE) and the residue was derivatized with pentafluoropropionic anhydride/pentafluoropropanol (PFPA/PFP). Gas chromatography-mass spectrometry (GC-MS) was used to detect the analytes in selected ion monitoring mode (SIM). Confidence parameters of validation of the method were: recovery, intra- and inter-assay precision, as well as limit of detection (LOD) of the analytes. The limits of detection were: 3.5 ng/mg for NCOC and 3.0 ng/mg for COC and BE. Good intra-assay precision was observed for all detected substances (coefficient of variation (CV)<11%). The inter-assay precision for norcocaine and benzoylecgonine were <4%. For intra- and inter-assay precision deuterated internal standards were used. Toenail and fingernail samples from eight declared cocaine users were submitted to the validated method. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/16183230/Validation_of_a_method_to_detect_cocaine_and_its_metabolites_in_nails_by_gas_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(05)00451-2 DB - PRIME DP - Unbound Medicine ER -