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Associations of high-risk HPV types and viral load with cervical cancer in China.
J Clin Virol 2006; 35(3):264-9JC

Abstract

BACKGROUND

Cervical cancer is the second most common cancer in women worldwide. Infection with some genotypes of human papillomavirus is the most important risk factor associated with cervical cancer.

OBJECTIVE

The aim of this study was to determine the prevalence and genotypes of HPV in China, and to evaluate the correlation between viral load of high risk HPV and cervical cancer and its precursors.

STUDY DESIGN

A cross-sectional study was carried out, wherein cervical samples were collected from 541 patients with cervical cancer, 262 with CIN, 139 with cervicitis and 68 age-matched healthy controls. Hybrid Capture 2 was employed to detect HPV DNA. Specimens from HPV DNA positive cervical cancer were tested for HPV types by using type specific PCR and general primer PCR with sequence-based typing (GP PCR-SBT).

RESULTS

Overall high risk HPV prevalence was 68.8% in CIN1, 80.3% in CIN2, 90.2% in CIN3, 90.9% in cervical cancer in situ, 89.9% in invasive cervical cancer and 25% in healthy controls from China. The most common HPV DNA type found in patients with cervical cancer was HPV16 (79.6%), followed by HPV58 (5.92%), HPV33 (3.29%), HPV18 (1.97%), HPV6 (1.97%), HPV31 (1.31%), HPV39 (1.31%), HPV68 (1.31%) and other HPV types (3.3%). It was found that there was a significantly increased risk of increasing CIN stage with high viral load. Frequency of low viral load found in the controls was 13.2% and 22.9% of CIN1, obtaining an OR of 4.2 (1.5-12.0). Associations (OR) among low viral load and CIN2/3, CIS, and CC were 6.7 (2.9-15.6), 9.4 (2.7-32.3) and 8.3 (3.7-18.4), respectively. While high viral loads were found in 5.9% of controls, 27.1% of CIN1, 42.1% of CIN2/3 and 48.5% of CIS, demonstrating increasing odds ratios with severity of disease (OR for CIS=68.0, 95% CI=17.8-259.7).

CONCLUSIONS

HPV16 was the most common genotype in central China. The developing cervical cancer precursors were associated with elevated high-risk HPV viral load.

Authors+Show Affiliations

State Key Laboratory of Biocontrol, Key Laboratory of Genetic Engineering of MOE, Department of Biochemistry, College of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, PR China. exwyp@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16183329

Citation

Wu, Yuping, et al. "Associations of High-risk HPV Types and Viral Load With Cervical Cancer in China." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 35, no. 3, 2006, pp. 264-9.
Wu Y, Chen Y, Li L, et al. Associations of high-risk HPV types and viral load with cervical cancer in China. J Clin Virol. 2006;35(3):264-9.
Wu, Y., Chen, Y., Li, L., Yu, G., Zhang, Y., & He, Y. (2006). Associations of high-risk HPV types and viral load with cervical cancer in China. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 35(3), pp. 264-9.
Wu Y, et al. Associations of High-risk HPV Types and Viral Load With Cervical Cancer in China. J Clin Virol. 2006;35(3):264-9. PubMed PMID: 16183329.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Associations of high-risk HPV types and viral load with cervical cancer in China. AU - Wu,Yuping, AU - Chen,Yulong, AU - Li,Longyu, AU - Yu,Guifang, AU - Zhang,Yanling, AU - He,Ying, Y1 - 2005/09/23/ PY - 2005/04/07/received PY - 2005/07/15/revised PY - 2005/07/30/accepted PY - 2005/9/27/pubmed PY - 2006/4/28/medline PY - 2005/9/27/entrez SP - 264 EP - 9 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J. Clin. Virol. VL - 35 IS - 3 N2 - BACKGROUND: Cervical cancer is the second most common cancer in women worldwide. Infection with some genotypes of human papillomavirus is the most important risk factor associated with cervical cancer. OBJECTIVE: The aim of this study was to determine the prevalence and genotypes of HPV in China, and to evaluate the correlation between viral load of high risk HPV and cervical cancer and its precursors. STUDY DESIGN: A cross-sectional study was carried out, wherein cervical samples were collected from 541 patients with cervical cancer, 262 with CIN, 139 with cervicitis and 68 age-matched healthy controls. Hybrid Capture 2 was employed to detect HPV DNA. Specimens from HPV DNA positive cervical cancer were tested for HPV types by using type specific PCR and general primer PCR with sequence-based typing (GP PCR-SBT). RESULTS: Overall high risk HPV prevalence was 68.8% in CIN1, 80.3% in CIN2, 90.2% in CIN3, 90.9% in cervical cancer in situ, 89.9% in invasive cervical cancer and 25% in healthy controls from China. The most common HPV DNA type found in patients with cervical cancer was HPV16 (79.6%), followed by HPV58 (5.92%), HPV33 (3.29%), HPV18 (1.97%), HPV6 (1.97%), HPV31 (1.31%), HPV39 (1.31%), HPV68 (1.31%) and other HPV types (3.3%). It was found that there was a significantly increased risk of increasing CIN stage with high viral load. Frequency of low viral load found in the controls was 13.2% and 22.9% of CIN1, obtaining an OR of 4.2 (1.5-12.0). Associations (OR) among low viral load and CIN2/3, CIS, and CC were 6.7 (2.9-15.6), 9.4 (2.7-32.3) and 8.3 (3.7-18.4), respectively. While high viral loads were found in 5.9% of controls, 27.1% of CIN1, 42.1% of CIN2/3 and 48.5% of CIS, demonstrating increasing odds ratios with severity of disease (OR for CIS=68.0, 95% CI=17.8-259.7). CONCLUSIONS: HPV16 was the most common genotype in central China. The developing cervical cancer precursors were associated with elevated high-risk HPV viral load. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/16183329/Associations_of_high_risk_HPV_types_and_viral_load_with_cervical_cancer_in_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(05)00232-5 DB - PRIME DP - Unbound Medicine ER -