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Fortifying fresh human milk with commercial powdered human milk fortifiers does not affect bacterial growth during 6 hours at room temperature.
J Am Diet Assoc. 2005 Oct; 105(10):1567-72.JA

Abstract

OBJECTIVE

To evaluate the growth of resident aerobic mesophilic flora and added Enterobacter sakazakii in fresh, unfortified human milk; fresh human milk fortified with two commercial powdered fortifiers differing in iron content; and infant formula prepared from powder.

SUBJECTS

Eight mothers provided preterm breast milk samples.

METHODS

Breast milk samples were divided into three aliquots: unfortified, fortified with fortifier containing 1.44 mg iron/14 kcal, and fortified with fortifier containing 0.4 mg iron/14 kcal. Aliquots of formula were prepared. Breast milk and formula aliquots were divided into two test samples. Half were inoculated with low amounts of E sakazakii; half were not. All test samples were maintained at room temperature (22 degrees C), serially diluted, and plated onto agars after 0, 2, 4, and 6 hours. Plates were incubated at 35 degrees C and enumerated.

STATISTICAL ANALYSES

Data were analyzed using repeated measures analysis of variance. P<.05 was considered significant.

RESULTS

There were no differences in colony counts of aerobic bacteria among uninoculated or among inoculated human milk samples at any time; counts did not increase significantly over 6 hours. There were no differences in colony counts of E sakazakii among inoculated human milk samples at any time; counts did not increase significantly over 6 hours. Aerobic bacteria and E sakazakii colony counts from infant formula did not increase significantly over 6 hours.

CONCLUSIONS

During 6 hours at 22 degrees C, fresh human milk and formula had negligible bacterial growth; fortifying human milk with powdered fortifiers did not affect bacterial growth.

Authors+Show Affiliations

Department of Medicine, University of Louisville, Louisville, KY, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16183356

Citation

Telang, Sucheta, et al. "Fortifying Fresh Human Milk With Commercial Powdered Human Milk Fortifiers Does Not Affect Bacterial Growth During 6 Hours at Room Temperature." Journal of the American Dietetic Association, vol. 105, no. 10, 2005, pp. 1567-72.
Telang S, Berseth CL, Ferguson PW, et al. Fortifying fresh human milk with commercial powdered human milk fortifiers does not affect bacterial growth during 6 hours at room temperature. J Am Diet Assoc. 2005;105(10):1567-72.
Telang, S., Berseth, C. L., Ferguson, P. W., Kinder, J. M., DeRoin, M., & Petschow, B. W. (2005). Fortifying fresh human milk with commercial powdered human milk fortifiers does not affect bacterial growth during 6 hours at room temperature. Journal of the American Dietetic Association, 105(10), 1567-72.
Telang S, et al. Fortifying Fresh Human Milk With Commercial Powdered Human Milk Fortifiers Does Not Affect Bacterial Growth During 6 Hours at Room Temperature. J Am Diet Assoc. 2005;105(10):1567-72. PubMed PMID: 16183356.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fortifying fresh human milk with commercial powdered human milk fortifiers does not affect bacterial growth during 6 hours at room temperature. AU - Telang,Sucheta, AU - Berseth,Carol Lynn, AU - Ferguson,Paul W, AU - Kinder,Julie M, AU - DeRoin,Mark, AU - Petschow,Bryon W, PY - 2004/10/18/received PY - 2005/9/27/pubmed PY - 2005/12/13/medline PY - 2005/9/27/entrez SP - 1567 EP - 72 JF - Journal of the American Dietetic Association JO - J Am Diet Assoc VL - 105 IS - 10 N2 - OBJECTIVE: To evaluate the growth of resident aerobic mesophilic flora and added Enterobacter sakazakii in fresh, unfortified human milk; fresh human milk fortified with two commercial powdered fortifiers differing in iron content; and infant formula prepared from powder. SUBJECTS: Eight mothers provided preterm breast milk samples. METHODS: Breast milk samples were divided into three aliquots: unfortified, fortified with fortifier containing 1.44 mg iron/14 kcal, and fortified with fortifier containing 0.4 mg iron/14 kcal. Aliquots of formula were prepared. Breast milk and formula aliquots were divided into two test samples. Half were inoculated with low amounts of E sakazakii; half were not. All test samples were maintained at room temperature (22 degrees C), serially diluted, and plated onto agars after 0, 2, 4, and 6 hours. Plates were incubated at 35 degrees C and enumerated. STATISTICAL ANALYSES: Data were analyzed using repeated measures analysis of variance. P<.05 was considered significant. RESULTS: There were no differences in colony counts of aerobic bacteria among uninoculated or among inoculated human milk samples at any time; counts did not increase significantly over 6 hours. There were no differences in colony counts of E sakazakii among inoculated human milk samples at any time; counts did not increase significantly over 6 hours. Aerobic bacteria and E sakazakii colony counts from infant formula did not increase significantly over 6 hours. CONCLUSIONS: During 6 hours at 22 degrees C, fresh human milk and formula had negligible bacterial growth; fortifying human milk with powdered fortifiers did not affect bacterial growth. SN - 0002-8223 UR - https://www.unboundmedicine.com/medline/citation/16183356/Fortifying_fresh_human_milk_with_commercial_powdered_human_milk_fortifiers_does_not_affect_bacterial_growth_during_6_hours_at_room_temperature_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002-8223(05)01214-9 DB - PRIME DP - Unbound Medicine ER -