Tags

Type your tag names separated by a space and hit enter

Inhibition of lipid peroxidation by IRFI 042, a vitamin E analogue, decreases monensin cardiotoxicity in chicks.
Toxicol Appl Pharmacol. 2005 Oct 15; 208(2):137-44.TA

Abstract

Monensin, a well-known ionophore antibiotic, may cause severe damage in myocardial cells. We investigated whether IRFI 042, a new analogue of vitamin E, may block lipid peroxidation in myocardial cells and in turn protect against monensin toxicity. Monensin toxicity was induced by repeated daily administration of the ionophore antibiotic (150 mg/kg/day for 7 days). Sham animals received by oral gavages only a saline solution and were used as controls. All animals were randomized to receive concomitantly by oral gavages IRFI 042 (20 mg/kg) or its vehicle. The experiment lasted 8 days. Survival rate, heart lipid peroxidation, studied by means of thiobarbituric acid-reactive substances (TBARs) levels, cardiac expression of endothelial nitric oxide (e-NOS) and histological analysis of the heart were performed. Monensin administration caused a decrease in survival rate. Mortality appeared following the second monensin injection and at day 7 caused a survival rate of 20%. Thereafter, no further mortality was observed. IRFI 042 administration improved survival rate. Injection of the ionophore antibiotic resulted in a marked cardiac lipid peroxidation and in a significant reduction in cardiac e-NOS message and protein expression. IRFI 042 decreased heart TBARs levels (Monensin + vehicle = 6.5 +/- 0.8 nmol/mg; Monensin + IRFI 042 = 3.2 +/- 1.1 nmol/mg; P < 0.001) and increased e-NOS message and protein expression. Histological analysis showed that IRFI 042 improved myocardial cells damage and enhanced the depressed e-NOS expression in chick heart samples following monensin administration. Our data suggest that IRFI 042 is a promising drug to reduce monensin cardio-toxicity in chicks.

Authors+Show Affiliations

Department of Veterinary Public Health, Section of Veterinary Pharmacology and Toxicology, University of Messina, Polo Universitario Annunziata, 98100 Messina, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16183387

Citation

Calò, Margherita, et al. "Inhibition of Lipid Peroxidation By IRFI 042, a Vitamin E Analogue, Decreases Monensin Cardiotoxicity in Chicks." Toxicology and Applied Pharmacology, vol. 208, no. 2, 2005, pp. 137-44.
Calò M, Altavilla D, Seminara P, et al. Inhibition of lipid peroxidation by IRFI 042, a vitamin E analogue, decreases monensin cardiotoxicity in chicks. Toxicol Appl Pharmacol. 2005;208(2):137-44.
Calò, M., Altavilla, D., Seminara, P., Marini, H., Minutoli, L., Bitto, A., Naccari, F., & Squadrito, F. (2005). Inhibition of lipid peroxidation by IRFI 042, a vitamin E analogue, decreases monensin cardiotoxicity in chicks. Toxicology and Applied Pharmacology, 208(2), 137-44.
Calò M, et al. Inhibition of Lipid Peroxidation By IRFI 042, a Vitamin E Analogue, Decreases Monensin Cardiotoxicity in Chicks. Toxicol Appl Pharmacol. 2005 Oct 15;208(2):137-44. PubMed PMID: 16183387.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of lipid peroxidation by IRFI 042, a vitamin E analogue, decreases monensin cardiotoxicity in chicks. AU - Calò,Margherita, AU - Altavilla,Domenica, AU - Seminara,Paolo, AU - Marini,Herbert, AU - Minutoli,Letteria, AU - Bitto,Alessandra, AU - Naccari,Francesco, AU - Squadrito,Francesco, PY - 2004/11/22/received PY - 2005/01/28/revised PY - 2005/01/29/accepted PY - 2005/9/27/pubmed PY - 2005/11/3/medline PY - 2005/9/27/entrez SP - 137 EP - 44 JF - Toxicology and applied pharmacology JO - Toxicol Appl Pharmacol VL - 208 IS - 2 N2 - Monensin, a well-known ionophore antibiotic, may cause severe damage in myocardial cells. We investigated whether IRFI 042, a new analogue of vitamin E, may block lipid peroxidation in myocardial cells and in turn protect against monensin toxicity. Monensin toxicity was induced by repeated daily administration of the ionophore antibiotic (150 mg/kg/day for 7 days). Sham animals received by oral gavages only a saline solution and were used as controls. All animals were randomized to receive concomitantly by oral gavages IRFI 042 (20 mg/kg) or its vehicle. The experiment lasted 8 days. Survival rate, heart lipid peroxidation, studied by means of thiobarbituric acid-reactive substances (TBARs) levels, cardiac expression of endothelial nitric oxide (e-NOS) and histological analysis of the heart were performed. Monensin administration caused a decrease in survival rate. Mortality appeared following the second monensin injection and at day 7 caused a survival rate of 20%. Thereafter, no further mortality was observed. IRFI 042 administration improved survival rate. Injection of the ionophore antibiotic resulted in a marked cardiac lipid peroxidation and in a significant reduction in cardiac e-NOS message and protein expression. IRFI 042 decreased heart TBARs levels (Monensin + vehicle = 6.5 +/- 0.8 nmol/mg; Monensin + IRFI 042 = 3.2 +/- 1.1 nmol/mg; P < 0.001) and increased e-NOS message and protein expression. Histological analysis showed that IRFI 042 improved myocardial cells damage and enhanced the depressed e-NOS expression in chick heart samples following monensin administration. Our data suggest that IRFI 042 is a promising drug to reduce monensin cardio-toxicity in chicks. SN - 0041-008X UR - https://www.unboundmedicine.com/medline/citation/16183387/Inhibition_of_lipid_peroxidation_by_IRFI_042_a_vitamin_E_analogue_decreases_monensin_cardiotoxicity_in_chicks_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-008X(05)00042-6 DB - PRIME DP - Unbound Medicine ER -