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Novel method for the quantification of inorganic polyphosphate (iPoP) in Saccharomyces cerevisiae shows dependence of iPoP content on the growth phase.
Arch Microbiol. 2005 Nov; 184(2):129-36.AM

Abstract

Inorganic polyphosphate (iPoP)-linear chains of up to hundreds of phosphate residues-is ubiquitous in nature and appears to be involved in many different cellular processes. In Saccharomyces cerevisiae, iPoP has been detected in high concentrations, especially after transfer of phosphate-deprived cells to a high-phosphate medium. Here, the dynamics of iPoP synthesis in yeast as a function of the growth phase as well as glucose and phosphate availability have been investigated. To address this question, a simple, fast and novel method for the quantification of iPoP from yeast was developed. Both the iPoP content during growth and the iPoP "overplus" were highest towards the end of the exponential phase, when glucose became limiting. Accumulation of iPoP during growth required excess of free phosphate, while the iPoP "overplus" was only observed after the shift from low- to high-phosphate medium. The newly developed iPoP quantification method and the knowledge about the dynamics of iPoP content during growth made it possible to define specific growth conditions for the analysis of iPoP levels. These experimental procedures will be essential for the large-scale analysis of various mutant strains or the comparison of different growth conditions.

Authors+Show Affiliations

Swiss Federal Institute of Technology (ETH) Zurich, Institute of Plant Sciences, Biochemistry and Physiology of Plants, Universitätsstr. 2, CH-8092 Zurich, Switzerland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16184370

Citation

Werner, Thomas P., et al. "Novel Method for the Quantification of Inorganic Polyphosphate (iPoP) in Saccharomyces Cerevisiae Shows Dependence of iPoP Content On the Growth Phase." Archives of Microbiology, vol. 184, no. 2, 2005, pp. 129-36.
Werner TP, Amrhein N, Freimoser FM. Novel method for the quantification of inorganic polyphosphate (iPoP) in Saccharomyces cerevisiae shows dependence of iPoP content on the growth phase. Arch Microbiol. 2005;184(2):129-36.
Werner, T. P., Amrhein, N., & Freimoser, F. M. (2005). Novel method for the quantification of inorganic polyphosphate (iPoP) in Saccharomyces cerevisiae shows dependence of iPoP content on the growth phase. Archives of Microbiology, 184(2), 129-36.
Werner TP, Amrhein N, Freimoser FM. Novel Method for the Quantification of Inorganic Polyphosphate (iPoP) in Saccharomyces Cerevisiae Shows Dependence of iPoP Content On the Growth Phase. Arch Microbiol. 2005;184(2):129-36. PubMed PMID: 16184370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel method for the quantification of inorganic polyphosphate (iPoP) in Saccharomyces cerevisiae shows dependence of iPoP content on the growth phase. AU - Werner,Thomas P, AU - Amrhein,Nikolaus, AU - Freimoser,Florian M, Y1 - 2005/10/21/ PY - 2005/04/28/received PY - 2005/08/01/accepted PY - 2005/07/01/revised PY - 2005/9/27/pubmed PY - 2006/2/4/medline PY - 2005/9/27/entrez SP - 129 EP - 36 JF - Archives of microbiology JO - Arch Microbiol VL - 184 IS - 2 N2 - Inorganic polyphosphate (iPoP)-linear chains of up to hundreds of phosphate residues-is ubiquitous in nature and appears to be involved in many different cellular processes. In Saccharomyces cerevisiae, iPoP has been detected in high concentrations, especially after transfer of phosphate-deprived cells to a high-phosphate medium. Here, the dynamics of iPoP synthesis in yeast as a function of the growth phase as well as glucose and phosphate availability have been investigated. To address this question, a simple, fast and novel method for the quantification of iPoP from yeast was developed. Both the iPoP content during growth and the iPoP "overplus" were highest towards the end of the exponential phase, when glucose became limiting. Accumulation of iPoP during growth required excess of free phosphate, while the iPoP "overplus" was only observed after the shift from low- to high-phosphate medium. The newly developed iPoP quantification method and the knowledge about the dynamics of iPoP content during growth made it possible to define specific growth conditions for the analysis of iPoP levels. These experimental procedures will be essential for the large-scale analysis of various mutant strains or the comparison of different growth conditions. SN - 0302-8933 UR - https://www.unboundmedicine.com/medline/citation/16184370/Novel_method_for_the_quantification_of_inorganic_polyphosphate__iPoP__in_Saccharomyces_cerevisiae_shows_dependence_of_iPoP_content_on_the_growth_phase_ DB - PRIME DP - Unbound Medicine ER -
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