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Comparison of diagnostic and relapse flow cytometry phenotypes in childhood acute lymphoblastic leukemia: implications for residual disease detection: a report from the children's oncology group.
Cytometry B Clin Cytom. 2005 Nov; 68(1):18-24.CB

Abstract

BACKGROUND

Flow cytometric analysis of minimal residual disease (MRD) depends on detecting phenotypically abnormal populations. However, little is known about how phenotypic shifts between diagnosis and relapse affect MRD detection in childhood acute lymphoid leukemia (ALL).

METHODS

We compared diagnostic and relapse bone marrow specimens in 42 children with precursor B-ALL studied with the two-tube panel CD19-APC/CD45-PerCP/CD10-PE/CD20-FITC and CD19-APC/CD45-PerCP/CD9-PE/CD34-FITC.

RESULTS

At least 29 cases had phenotypic shifts of intensity or coefficient of variation of distribution of one or more markers. Shifts were complex and could not be explained by change in maturation stage. In the majority of cases MRD populations more closely resembled the diagnostic than the relapse specimen. In 6 of 7 MRD negative cases we did not identify an abnormal population that resembled diagnosis or relapse. In the remaining case, in which CD34 and CD10 were lost between diagnosis and relapse, it is possible that we could have missed an MRD population resembling relapse.

CONCLUSIONS

Phenotypic shifts are common, but do not affect MRD recognition. At most 1 of 42 cases might have harbored an abnormal population undetected because of shift. However, MRD analysis with rigid gating (looking strictly for abnormal phenotypes at diagnosis) might have missed many positive cases, 8 of 22 (36%) in this series.

Authors+Show Affiliations

Department of Pathology and Oncology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA. mborowit@jhmi.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16184615

Citation

Borowitz, Michael J., et al. "Comparison of Diagnostic and Relapse Flow Cytometry Phenotypes in Childhood Acute Lymphoblastic Leukemia: Implications for Residual Disease Detection: a Report From the Children's Oncology Group." Cytometry. Part B, Clinical Cytometry, vol. 68, no. 1, 2005, pp. 18-24.
Borowitz MJ, Pullen DJ, Winick N, et al. Comparison of diagnostic and relapse flow cytometry phenotypes in childhood acute lymphoblastic leukemia: implications for residual disease detection: a report from the children's oncology group. Cytometry B Clin Cytom. 2005;68(1):18-24.
Borowitz, M. J., Pullen, D. J., Winick, N., Martin, P. L., Bowman, W. P., & Camitta, B. (2005). Comparison of diagnostic and relapse flow cytometry phenotypes in childhood acute lymphoblastic leukemia: implications for residual disease detection: a report from the children's oncology group. Cytometry. Part B, Clinical Cytometry, 68(1), 18-24.
Borowitz MJ, et al. Comparison of Diagnostic and Relapse Flow Cytometry Phenotypes in Childhood Acute Lymphoblastic Leukemia: Implications for Residual Disease Detection: a Report From the Children's Oncology Group. Cytometry B Clin Cytom. 2005;68(1):18-24. PubMed PMID: 16184615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of diagnostic and relapse flow cytometry phenotypes in childhood acute lymphoblastic leukemia: implications for residual disease detection: a report from the children's oncology group. AU - Borowitz,Michael J, AU - Pullen,D Jeanette, AU - Winick,Naomi, AU - Martin,Paul L, AU - Bowman,W Paul, AU - Camitta,Bruce, PY - 2005/9/27/pubmed PY - 2006/2/17/medline PY - 2005/9/27/entrez SP - 18 EP - 24 JF - Cytometry. Part B, Clinical cytometry JO - Cytometry B Clin Cytom VL - 68 IS - 1 N2 - BACKGROUND: Flow cytometric analysis of minimal residual disease (MRD) depends on detecting phenotypically abnormal populations. However, little is known about how phenotypic shifts between diagnosis and relapse affect MRD detection in childhood acute lymphoid leukemia (ALL). METHODS: We compared diagnostic and relapse bone marrow specimens in 42 children with precursor B-ALL studied with the two-tube panel CD19-APC/CD45-PerCP/CD10-PE/CD20-FITC and CD19-APC/CD45-PerCP/CD9-PE/CD34-FITC. RESULTS: At least 29 cases had phenotypic shifts of intensity or coefficient of variation of distribution of one or more markers. Shifts were complex and could not be explained by change in maturation stage. In the majority of cases MRD populations more closely resembled the diagnostic than the relapse specimen. In 6 of 7 MRD negative cases we did not identify an abnormal population that resembled diagnosis or relapse. In the remaining case, in which CD34 and CD10 were lost between diagnosis and relapse, it is possible that we could have missed an MRD population resembling relapse. CONCLUSIONS: Phenotypic shifts are common, but do not affect MRD recognition. At most 1 of 42 cases might have harbored an abnormal population undetected because of shift. However, MRD analysis with rigid gating (looking strictly for abnormal phenotypes at diagnosis) might have missed many positive cases, 8 of 22 (36%) in this series. SN - 1552-4949 UR - https://www.unboundmedicine.com/medline/citation/16184615/Comparison_of_diagnostic_and_relapse_flow_cytometry_phenotypes_in_childhood_acute_lymphoblastic_leukemia:_implications_for_residual_disease_detection:_a_report_from_the_children's_oncology_group_ L2 - https://doi.org/10.1002/cyto.b.20071 DB - PRIME DP - Unbound Medicine ER -