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Measles immunity testing: comparison of two measles IgG ELISAs with plaque reduction neutralisation assay.
J Virol Methods. 2006 Feb; 131(2):209-12.JV

Abstract

Two commercial IgG ELISAs, one based on recombinant nucleocapsid antigen and one based on cell culture grown native virus antigens, were evaluated for measles immunity testing by comparison with plaque reduction neutralisation test (PRNT). Qualitative results of the two ELISAs showed 92% agreement with those of PRNT. The sensitivity of the two ELISAs was 89.6%. False negative ELISA results were obtained in 10% of sera, mainly sera containing low levels of neutralising antibody. The specificity of both ELISAs was 100%. Measles IgG ELISAs perform adequately for immunity testing, correctly identifying seronegative individuals for vaccination.

Authors+Show Affiliations

Virus Reference Department, Centre for Infections, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK. Bernard.cohen@hpa.org.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16188328

Citation

Cohen, B J., et al. "Measles Immunity Testing: Comparison of Two Measles IgG ELISAs With Plaque Reduction Neutralisation Assay." Journal of Virological Methods, vol. 131, no. 2, 2006, pp. 209-12.
Cohen BJ, Parry RP, Doblas D, et al. Measles immunity testing: comparison of two measles IgG ELISAs with plaque reduction neutralisation assay. J Virol Methods. 2006;131(2):209-12.
Cohen, B. J., Parry, R. P., Doblas, D., Samuel, D., Warrener, L., Andrews, N., & Brown, D. (2006). Measles immunity testing: comparison of two measles IgG ELISAs with plaque reduction neutralisation assay. Journal of Virological Methods, 131(2), 209-12.
Cohen BJ, et al. Measles Immunity Testing: Comparison of Two Measles IgG ELISAs With Plaque Reduction Neutralisation Assay. J Virol Methods. 2006;131(2):209-12. PubMed PMID: 16188328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Measles immunity testing: comparison of two measles IgG ELISAs with plaque reduction neutralisation assay. AU - Cohen,B J, AU - Parry,R P, AU - Doblas,D, AU - Samuel,D, AU - Warrener,L, AU - Andrews,N, AU - Brown,D, Y1 - 2005/09/26/ PY - 2005/06/08/received PY - 2005/08/08/revised PY - 2005/08/08/accepted PY - 2005/9/29/pubmed PY - 2006/7/11/medline PY - 2005/9/29/entrez SP - 209 EP - 12 JF - Journal of virological methods JO - J Virol Methods VL - 131 IS - 2 N2 - Two commercial IgG ELISAs, one based on recombinant nucleocapsid antigen and one based on cell culture grown native virus antigens, were evaluated for measles immunity testing by comparison with plaque reduction neutralisation test (PRNT). Qualitative results of the two ELISAs showed 92% agreement with those of PRNT. The sensitivity of the two ELISAs was 89.6%. False negative ELISA results were obtained in 10% of sera, mainly sera containing low levels of neutralising antibody. The specificity of both ELISAs was 100%. Measles IgG ELISAs perform adequately for immunity testing, correctly identifying seronegative individuals for vaccination. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/16188328/Measles_immunity_testing:_comparison_of_two_measles_IgG_ELISAs_with_plaque_reduction_neutralisation_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-0934(05)00261-2 DB - PRIME DP - Unbound Medicine ER -