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A practical approach to the clinical diagnosis of Ewing's sarcoma/primitive neuroectodermal tumour and other small round cell tumours sharing EWS rearrangement using new fluorescence in situ hybridisation probes for EWSR1 on formalin fixed, paraffin wax embedded tissue.
J Clin Pathol. 2005 Oct; 58(10):1051-6.JC

Abstract

BACKGROUND

Over 90% of Ewing's sarcoma/primitive neuroectodermal tumour (ES/PNET) cases have the t(11;22) chromosomal rearrangement, which is also found in other small round cell tumours, including desmoplastic small round cell tumour (DSRCT) and clear cell sarcoma (CCS). Although this rearrangement can be analysed by fluorescence in situ hybridisation (FISH) using routinely formalin fixed, paraffin wax embedded (FFPE) tissues when fresh or frozen tissues are not available, a sensitive and convenient detection method is needed for routine clinical diagnosis.

AIMS

To investigate the usefulness of newly developed probes for detecting EWS rearrangement resulting from chromosomal translocations using FISH and FFPE tissue in the clinical diagnosis of ES/PNET, DSRCT, and CCS.

METHODS

Sixteen ES/PNETs, six DSRCTs, and six CCSs were studied. Three poorly differentiated synovial sarcomas, three alveolar rhabdomyosarcomas, and three neuroblastomas served as negative controls. Interphase FISH analysis was performed on FFPE tissue sections with a commercially available EWSR1 (22q12) dual colour, breakapart rearrangement probe.

RESULTS

One fused signal and one split signal of orange and green, demonstrating rearrangement of the EWS gene, was detected in 14 of 16 ES/PNETs, all six DRSCTs, and five of six CCSs, but not in the negative controls.

CONCLUSIONS

Interphase FISH using this newly developed probe is sensitive and specific for detecting the EWS gene on FFPE tissues and is of value in the routine clinical diagnosis of ES/PNET, DSRCT, and CCS.

Authors+Show Affiliations

Division of Orthopaedic Oncology, National Cancer Centre Hospital and Research Institute, Tokyo 104-0045, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16189150

Citation

Yamaguchi, U, et al. "A Practical Approach to the Clinical Diagnosis of Ewing's Sarcoma/primitive Neuroectodermal Tumour and Other Small Round Cell Tumours Sharing EWS Rearrangement Using New Fluorescence in Situ Hybridisation Probes for EWSR1 On Formalin Fixed, Paraffin Wax Embedded Tissue." Journal of Clinical Pathology, vol. 58, no. 10, 2005, pp. 1051-6.
Yamaguchi U, Hasegawa T, Morimoto Y, et al. A practical approach to the clinical diagnosis of Ewing's sarcoma/primitive neuroectodermal tumour and other small round cell tumours sharing EWS rearrangement using new fluorescence in situ hybridisation probes for EWSR1 on formalin fixed, paraffin wax embedded tissue. J Clin Pathol. 2005;58(10):1051-6.
Yamaguchi, U., Hasegawa, T., Morimoto, Y., Tateishi, U., Endo, M., Nakatani, F., Kawai, A., Chuman, H., Beppu, Y., Endo, M., Kurotaki, H., & Furuta, K. (2005). A practical approach to the clinical diagnosis of Ewing's sarcoma/primitive neuroectodermal tumour and other small round cell tumours sharing EWS rearrangement using new fluorescence in situ hybridisation probes for EWSR1 on formalin fixed, paraffin wax embedded tissue. Journal of Clinical Pathology, 58(10), 1051-6.
Yamaguchi U, et al. A Practical Approach to the Clinical Diagnosis of Ewing's Sarcoma/primitive Neuroectodermal Tumour and Other Small Round Cell Tumours Sharing EWS Rearrangement Using New Fluorescence in Situ Hybridisation Probes for EWSR1 On Formalin Fixed, Paraffin Wax Embedded Tissue. J Clin Pathol. 2005;58(10):1051-6. PubMed PMID: 16189150.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A practical approach to the clinical diagnosis of Ewing's sarcoma/primitive neuroectodermal tumour and other small round cell tumours sharing EWS rearrangement using new fluorescence in situ hybridisation probes for EWSR1 on formalin fixed, paraffin wax embedded tissue. AU - Yamaguchi,U, AU - Hasegawa,T, AU - Morimoto,Y, AU - Tateishi,U, AU - Endo,M, AU - Nakatani,F, AU - Kawai,A, AU - Chuman,H, AU - Beppu,Y, AU - Endo,M, AU - Kurotaki,H, AU - Furuta,K, PY - 2005/9/29/pubmed PY - 2005/11/16/medline PY - 2005/9/29/entrez SP - 1051 EP - 6 JF - Journal of clinical pathology JO - J. Clin. Pathol. VL - 58 IS - 10 N2 - BACKGROUND: Over 90% of Ewing's sarcoma/primitive neuroectodermal tumour (ES/PNET) cases have the t(11;22) chromosomal rearrangement, which is also found in other small round cell tumours, including desmoplastic small round cell tumour (DSRCT) and clear cell sarcoma (CCS). Although this rearrangement can be analysed by fluorescence in situ hybridisation (FISH) using routinely formalin fixed, paraffin wax embedded (FFPE) tissues when fresh or frozen tissues are not available, a sensitive and convenient detection method is needed for routine clinical diagnosis. AIMS: To investigate the usefulness of newly developed probes for detecting EWS rearrangement resulting from chromosomal translocations using FISH and FFPE tissue in the clinical diagnosis of ES/PNET, DSRCT, and CCS. METHODS: Sixteen ES/PNETs, six DSRCTs, and six CCSs were studied. Three poorly differentiated synovial sarcomas, three alveolar rhabdomyosarcomas, and three neuroblastomas served as negative controls. Interphase FISH analysis was performed on FFPE tissue sections with a commercially available EWSR1 (22q12) dual colour, breakapart rearrangement probe. RESULTS: One fused signal and one split signal of orange and green, demonstrating rearrangement of the EWS gene, was detected in 14 of 16 ES/PNETs, all six DRSCTs, and five of six CCSs, but not in the negative controls. CONCLUSIONS: Interphase FISH using this newly developed probe is sensitive and specific for detecting the EWS gene on FFPE tissues and is of value in the routine clinical diagnosis of ES/PNET, DSRCT, and CCS. SN - 0021-9746 UR - https://www.unboundmedicine.com/medline/citation/16189150/A_practical_approach_to_the_clinical_diagnosis_of_Ewing's_sarcoma/primitive_neuroectodermal_tumour_and_other_small_round_cell_tumours_sharing_EWS_rearrangement_using_new_fluorescence_in_situ_hybridisation_probes_for_EWSR1_on_formalin_fixed_paraffin_wax_embedded_tissue_ L2 - http://jcp.bmj.com/cgi/pmidlookup?view=long&pmid=16189150 DB - PRIME DP - Unbound Medicine ER -