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Reusable impedimetric aptasensor.
Anal Chem. 2005 Oct 01; 77(19):6320-3.AC

Abstract

A novel impedimetric aptasensor using a mixed self-assembled monolayer composed of thiol-modified thrombin binding aptamer and 2-mercaptoethanol on a gold electrode is reported. The changes of interfacial features of the electrode were probed in the presence of the reversible redox couple, Fe(CN)6(3-/4-), using impedance measurements. The electrode surface was partially blocked due to the self-assembly of aptamer or the formation of the aptamer-thrombin complex, resulting in an increase of the interfacial electron-transfer resistance detected by electrochemical impedance spectroscopy or cyclic voltammetry. The aptasensor was regenerated by breaking the complex formed between the aptamer and thrombin using 2.0 M NaCl solution, and the immobilized aptamer subsequently was used for repeated detection of thrombin. The aptamer-functionalized electrode showed a linear response of the charge-transfer resistance to the increase of thrombin concentration in the range of 5.0-35.0 nM and the thrombin was easily detectable to a concentration of 2.0 nM.

Authors+Show Affiliations

Radi AE
Department of Chemistry, Faculty of Science, Mansoura University, 34517 Dumyat, Egypt. abd.radi@urv.net
Acero Sánchez JL
No affiliation info available
Baldrich E
No affiliation info available
O'Sullivan CK
No affiliation info available

MeSH

Aptamers, NucleotideBiosensing TechniquesElectric ImpedanceElectrochemistryElectrodes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16194094

Citation

Radi, Abd-Elgawad, et al. "Reusable Impedimetric Aptasensor." Analytical Chemistry, vol. 77, no. 19, 2005, pp. 6320-3.
Radi AE, Acero Sánchez JL, Baldrich E, et al. Reusable impedimetric aptasensor. Anal Chem. 2005;77(19):6320-3.
Radi, A. E., Acero Sánchez, J. L., Baldrich, E., & O'Sullivan, C. K. (2005). Reusable impedimetric aptasensor. Analytical Chemistry, 77(19), 6320-3.
Radi AE, et al. Reusable Impedimetric Aptasensor. Anal Chem. 2005 Oct 1;77(19):6320-3. PubMed PMID: 16194094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reusable impedimetric aptasensor. AU - Radi,Abd-Elgawad, AU - Acero Sánchez,Josep Lluís, AU - Baldrich,Eva, AU - O'Sullivan,Ciara K, PY - 2005/10/1/pubmed PY - 2007/3/28/medline PY - 2005/10/1/entrez SP - 6320 EP - 3 JF - Analytical chemistry JO - Anal Chem VL - 77 IS - 19 N2 - A novel impedimetric aptasensor using a mixed self-assembled monolayer composed of thiol-modified thrombin binding aptamer and 2-mercaptoethanol on a gold electrode is reported. The changes of interfacial features of the electrode were probed in the presence of the reversible redox couple, Fe(CN)6(3-/4-), using impedance measurements. The electrode surface was partially blocked due to the self-assembly of aptamer or the formation of the aptamer-thrombin complex, resulting in an increase of the interfacial electron-transfer resistance detected by electrochemical impedance spectroscopy or cyclic voltammetry. The aptasensor was regenerated by breaking the complex formed between the aptamer and thrombin using 2.0 M NaCl solution, and the immobilized aptamer subsequently was used for repeated detection of thrombin. The aptamer-functionalized electrode showed a linear response of the charge-transfer resistance to the increase of thrombin concentration in the range of 5.0-35.0 nM and the thrombin was easily detectable to a concentration of 2.0 nM. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/16194094/Reusable_impedimetric_aptasensor_ DB - PRIME DP - Unbound Medicine ER -