Tags

Type your tag names separated by a space and hit enter

Preliminary characterisation of repeat families in the genome of EhV-86, a giant algal virus that infects the marine microalga Emiliania huxleyi.
Arch Virol. 2006 Mar; 151(3):525-35.AV

Abstract

EhV-86 is a large double stranded DNA virus with a 407,339 base pair circular genome that infects the globally important microalga Emiliania huxleyi. It belongs to a new genus of viruses termed the Coccolithoviridae within the algal virus family Phycodnaviridae. By plotting the EhV-86 genome against itself in a dot-plot analysis we revealed three families of distinctly different repeat sequences throughout its genome, designated Family A, B and C. Family A repeats are non-coding, found immediately upstream of 86 predicted coding sequences (CDSs) and are likely to play a crucial role in controlling the expression of the associated CDSs. Family B repeats are GC rich, coding and correspond to possible calcium binding sites in 22 proline-rich domains found in the protein products of eight predicted EhV-86 CDSs. Family C repeats are AT-rich, non-coding and are likely to form part of the origin of replication. We suggest that these repeat regions are of fundamental importance during virus propagation being involved with transcriptional control (Family A), virus adsorption/release (Family B) and DNA replication (Family C).

Authors+Show Affiliations

Plymouth Marine Laboratory, Plymouth, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16195784

Citation

Allen, M J., et al. "Preliminary Characterisation of Repeat Families in the Genome of EhV-86, a Giant Algal Virus That Infects the Marine Microalga Emiliania Huxleyi." Archives of Virology, vol. 151, no. 3, 2006, pp. 525-35.
Allen MJ, Schroeder DC, Wilson WH. Preliminary characterisation of repeat families in the genome of EhV-86, a giant algal virus that infects the marine microalga Emiliania huxleyi. Arch Virol. 2006;151(3):525-35.
Allen, M. J., Schroeder, D. C., & Wilson, W. H. (2006). Preliminary characterisation of repeat families in the genome of EhV-86, a giant algal virus that infects the marine microalga Emiliania huxleyi. Archives of Virology, 151(3), 525-35.
Allen MJ, Schroeder DC, Wilson WH. Preliminary Characterisation of Repeat Families in the Genome of EhV-86, a Giant Algal Virus That Infects the Marine Microalga Emiliania Huxleyi. Arch Virol. 2006;151(3):525-35. PubMed PMID: 16195784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preliminary characterisation of repeat families in the genome of EhV-86, a giant algal virus that infects the marine microalga Emiliania huxleyi. AU - Allen,M J, AU - Schroeder,D C, AU - Wilson,W H, Y1 - 2005/09/30/ PY - 2005/06/09/received PY - 2005/08/08/accepted PY - 2005/10/1/pubmed PY - 2006/5/4/medline PY - 2005/10/1/entrez SP - 525 EP - 35 JF - Archives of virology JO - Arch Virol VL - 151 IS - 3 N2 - EhV-86 is a large double stranded DNA virus with a 407,339 base pair circular genome that infects the globally important microalga Emiliania huxleyi. It belongs to a new genus of viruses termed the Coccolithoviridae within the algal virus family Phycodnaviridae. By plotting the EhV-86 genome against itself in a dot-plot analysis we revealed three families of distinctly different repeat sequences throughout its genome, designated Family A, B and C. Family A repeats are non-coding, found immediately upstream of 86 predicted coding sequences (CDSs) and are likely to play a crucial role in controlling the expression of the associated CDSs. Family B repeats are GC rich, coding and correspond to possible calcium binding sites in 22 proline-rich domains found in the protein products of eight predicted EhV-86 CDSs. Family C repeats are AT-rich, non-coding and are likely to form part of the origin of replication. We suggest that these repeat regions are of fundamental importance during virus propagation being involved with transcriptional control (Family A), virus adsorption/release (Family B) and DNA replication (Family C). SN - 0304-8608 UR - https://www.unboundmedicine.com/medline/citation/16195784/Preliminary_characterisation_of_repeat_families_in_the_genome_of_EhV_86_a_giant_algal_virus_that_infects_the_marine_microalga_Emiliania_huxleyi_ L2 - https://dx.doi.org/10.1007/s00705-005-0647-1 DB - PRIME DP - Unbound Medicine ER -