Tags

Type your tag names separated by a space and hit enter

Effects of dietary supplementation of rapeseed oil on metabolism of [1-14C]18:1n-9, [1-14C]20:3n-6, and [1-14C]20:4n-3 in Atlantic salmon hepatocytes.
Lipids. 2005 Jul; 40(7):709-17.L

Abstract

Atlantic salmon were fed fish meal-based diets supplemented with either 100% fish oil (FO) or 100% rapeseed oil (RO) from an initial weight of 85 g to a final average weight of 280 g. The effects of these diets on the capacity of Atlantic salmon hepatocytes to elongate, desaturate, and esterify [1-14C] 18:1n-9 and the immediate substrates for the delta5 desaturase, [1-14C] 20:3 n-6 and [1-14C] 20:4n-3, were investigated. Radiolabeled 18:1n-9 was mainly esterified into cellular TAG, whereas the more polyunsaturated FA, [1-14C] 20:3n-6 and [1-14C] 20:4n-3, were primarily esterified into cellular PL. More of the elongation product, [1-14C] 20:1n-9, was produced from 18:1n-9 and more of the desaturation and elongation products, 22:5n-6 and 22:6n-3, were produced from [1-14C]20:3n-6 and [1-14C] 20:4n-3, respectively, in RO hepatocytes than in FO hepatocytes. Further, we studied whether increased addition of [1-14C]18:1n-9 to the hepatocyte culture media would affect the capacity of hepatocytes to oxidize 18:1n-9 to acid-soluble products and CO2. An increase in exogenous concentration of 18:1 n-9 from 7 to 100 microM resulted in a nearly twofold increase in the amount of 18:1n-9 that was oxidized. The conversion of 20:4n-3 and 20:3n-6 to the longer-chain 22:6n-3 and 22:5n-6 was enhanced by RO feeding in Atlantic salmon hepatocytes. The increased capacity of RO hepatocytes to produce 22:6n-3 was, however, not enough to achieve the levels found in FO hepatocytes. Our data further showed that there were no differences in the hepatocyte FA oxidation capacity and the lipid deposition of carcass and liver between the two groups.

Authors+Show Affiliations

AKVAFORSK, Institute of Aquaculture Research, NO-1432 As, Norway. corina.moya-falcon@akvaforsk.noNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16196422

Citation

Moya-Falcón, C, et al. "Effects of Dietary Supplementation of Rapeseed Oil On Metabolism of [1-14C]18:1n-9, [1-14C]20:3n-6, and [1-14C]20:4n-3 in Atlantic Salmon Hepatocytes." Lipids, vol. 40, no. 7, 2005, pp. 709-17.
Moya-Falcón C, Thomassen MS, Jakobsen JV, et al. Effects of dietary supplementation of rapeseed oil on metabolism of [1-14C]18:1n-9, [1-14C]20:3n-6, and [1-14C]20:4n-3 in Atlantic salmon hepatocytes. Lipids. 2005;40(7):709-17.
Moya-Falcón, C., Thomassen, M. S., Jakobsen, J. V., & Ruyter, B. (2005). Effects of dietary supplementation of rapeseed oil on metabolism of [1-14C]18:1n-9, [1-14C]20:3n-6, and [1-14C]20:4n-3 in Atlantic salmon hepatocytes. Lipids, 40(7), 709-17.
Moya-Falcón C, et al. Effects of Dietary Supplementation of Rapeseed Oil On Metabolism of [1-14C]18:1n-9, [1-14C]20:3n-6, and [1-14C]20:4n-3 in Atlantic Salmon Hepatocytes. Lipids. 2005;40(7):709-17. PubMed PMID: 16196422.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary supplementation of rapeseed oil on metabolism of [1-14C]18:1n-9, [1-14C]20:3n-6, and [1-14C]20:4n-3 in Atlantic salmon hepatocytes. AU - Moya-Falcón,C, AU - Thomassen,M S, AU - Jakobsen,J V, AU - Ruyter,B, PY - 2005/10/4/pubmed PY - 2006/1/24/medline PY - 2005/10/4/entrez SP - 709 EP - 17 JF - Lipids JO - Lipids VL - 40 IS - 7 N2 - Atlantic salmon were fed fish meal-based diets supplemented with either 100% fish oil (FO) or 100% rapeseed oil (RO) from an initial weight of 85 g to a final average weight of 280 g. The effects of these diets on the capacity of Atlantic salmon hepatocytes to elongate, desaturate, and esterify [1-14C] 18:1n-9 and the immediate substrates for the delta5 desaturase, [1-14C] 20:3 n-6 and [1-14C] 20:4n-3, were investigated. Radiolabeled 18:1n-9 was mainly esterified into cellular TAG, whereas the more polyunsaturated FA, [1-14C] 20:3n-6 and [1-14C] 20:4n-3, were primarily esterified into cellular PL. More of the elongation product, [1-14C] 20:1n-9, was produced from 18:1n-9 and more of the desaturation and elongation products, 22:5n-6 and 22:6n-3, were produced from [1-14C]20:3n-6 and [1-14C] 20:4n-3, respectively, in RO hepatocytes than in FO hepatocytes. Further, we studied whether increased addition of [1-14C]18:1n-9 to the hepatocyte culture media would affect the capacity of hepatocytes to oxidize 18:1n-9 to acid-soluble products and CO2. An increase in exogenous concentration of 18:1 n-9 from 7 to 100 microM resulted in a nearly twofold increase in the amount of 18:1n-9 that was oxidized. The conversion of 20:4n-3 and 20:3n-6 to the longer-chain 22:6n-3 and 22:5n-6 was enhanced by RO feeding in Atlantic salmon hepatocytes. The increased capacity of RO hepatocytes to produce 22:6n-3 was, however, not enough to achieve the levels found in FO hepatocytes. Our data further showed that there were no differences in the hepatocyte FA oxidation capacity and the lipid deposition of carcass and liver between the two groups. SN - 0024-4201 UR - https://www.unboundmedicine.com/medline/citation/16196422/Effects_of_dietary_supplementation_of_rapeseed_oil_on_metabolism_of_[1_14C]18:1n_9_[1_14C]20:3n_6_and_[1_14C]20:4n_3_in_Atlantic_salmon_hepatocytes_ L2 - https://link.springer.com/article/10.1007/s11745-005-1434-9 DB - PRIME DP - Unbound Medicine ER -