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Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen.

Abstract

BACKGROUND

Orange is among the most widely consumed fruits, and among the plant food sources causing allergic reactions according to popular perception. However, its relevant allergenic components are virtually unknown. Profilin is a well-defined minor plant panallergen, showing prevalences around 30% in fruits and vegetables.

METHODS

Twenty-three orange-allergic patients were studied. Natural orange profilin, named Cit s 2, was purified by affinity chromatography and characterized by N-terminal amino acid sequencing, matrix-assisted laser desorption/ionization mass spectrometry analysis and isolation of its coding cDNA. Reactivity to Cit s 2 was analyzed in vivo by skin prick tests (SPT) and in vitro by IgE immunodetection, specific IgE determination in individual sera and enzyme-linked immunosorbent assay-inhibition assays.

RESULTS

The N-terminal amino acid sequence and molecular mass of natural Cit s 2, both fully in agreement with the complete amino acid sequence deduced from its coding cDNA, demonstrated its profilin nature. An unexpectedly high reactivity to Cit s 2 was found in vivo (78% of positive SPT responses) and in vitro (87% of sera from orange allergic patients had specific IgE to Cit s 2). The purified allergen inhibited around 50% of the IgE binding to an orange pulp extract.

CONCLUSION

Orange profilin Cit s 2, unlike other plant food profilins, is a major and highly prevalent allergen.

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  • Authors+Show Affiliations

    ,

    Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, Madrid, Spain.

    , , , , , ,

    Source

    Allergy 60:11 2005 Nov pg 1424-9

    MeSH

    Adolescent
    Adult
    Allergens
    Amino Acid Sequence
    Antibody Specificity
    Antigens, Plant
    Child
    Chromatography, Affinity
    Citrus sinensis
    Female
    Food Hypersensitivity
    Humans
    Immunoglobulin E
    Infant
    Male
    Molecular Sequence Data
    Molecular Weight
    Plant Proteins
    Profilins
    Sequence Alignment
    Skin Tests

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    16197476

    Citation

    López-Torrejón, G, et al. "Isolation, Cloning and Allergenic Reactivity of Natural Profilin Cit S 2, a Major Orange Allergen." Allergy, vol. 60, no. 11, 2005, pp. 1424-9.
    López-Torrejón G, Ibáñez MD, Ahrazem O, et al. Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen. Allergy. 2005;60(11):1424-9.
    López-Torrejón, G., Ibáñez, M. D., Ahrazem, O., Sánchez-Monge, R., Sastre, J., Lombardero, M., ... Salcedo, G. (2005). Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen. Allergy, 60(11), pp. 1424-9.
    López-Torrejón G, et al. Isolation, Cloning and Allergenic Reactivity of Natural Profilin Cit S 2, a Major Orange Allergen. Allergy. 2005;60(11):1424-9. PubMed PMID: 16197476.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen. AU - López-Torrejón,G, AU - Ibáñez,M D, AU - Ahrazem,O, AU - Sánchez-Monge,R, AU - Sastre,J, AU - Lombardero,M, AU - Barber,D, AU - Salcedo,G, PY - 2005/10/4/pubmed PY - 2006/3/24/medline PY - 2005/10/4/entrez SP - 1424 EP - 9 JF - Allergy JO - Allergy VL - 60 IS - 11 N2 - BACKGROUND: Orange is among the most widely consumed fruits, and among the plant food sources causing allergic reactions according to popular perception. However, its relevant allergenic components are virtually unknown. Profilin is a well-defined minor plant panallergen, showing prevalences around 30% in fruits and vegetables. METHODS: Twenty-three orange-allergic patients were studied. Natural orange profilin, named Cit s 2, was purified by affinity chromatography and characterized by N-terminal amino acid sequencing, matrix-assisted laser desorption/ionization mass spectrometry analysis and isolation of its coding cDNA. Reactivity to Cit s 2 was analyzed in vivo by skin prick tests (SPT) and in vitro by IgE immunodetection, specific IgE determination in individual sera and enzyme-linked immunosorbent assay-inhibition assays. RESULTS: The N-terminal amino acid sequence and molecular mass of natural Cit s 2, both fully in agreement with the complete amino acid sequence deduced from its coding cDNA, demonstrated its profilin nature. An unexpectedly high reactivity to Cit s 2 was found in vivo (78% of positive SPT responses) and in vitro (87% of sera from orange allergic patients had specific IgE to Cit s 2). The purified allergen inhibited around 50% of the IgE binding to an orange pulp extract. CONCLUSION: Orange profilin Cit s 2, unlike other plant food profilins, is a major and highly prevalent allergen. SN - 0105-4538 UR - https://www.unboundmedicine.com/medline/citation/16197476/Isolation_cloning_and_allergenic_reactivity_of_natural_profilin_Cit_s_2_a_major_orange_allergen_ L2 - https://doi.org/10.1111/j.1398-9995.2005.00903.x DB - PRIME DP - Unbound Medicine ER -