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Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen.
Allergy 2005; 60(11):1424-9A

Abstract

BACKGROUND

Orange is among the most widely consumed fruits, and among the plant food sources causing allergic reactions according to popular perception. However, its relevant allergenic components are virtually unknown. Profilin is a well-defined minor plant panallergen, showing prevalences around 30% in fruits and vegetables.

METHODS

Twenty-three orange-allergic patients were studied. Natural orange profilin, named Cit s 2, was purified by affinity chromatography and characterized by N-terminal amino acid sequencing, matrix-assisted laser desorption/ionization mass spectrometry analysis and isolation of its coding cDNA. Reactivity to Cit s 2 was analyzed in vivo by skin prick tests (SPT) and in vitro by IgE immunodetection, specific IgE determination in individual sera and enzyme-linked immunosorbent assay-inhibition assays.

RESULTS

The N-terminal amino acid sequence and molecular mass of natural Cit s 2, both fully in agreement with the complete amino acid sequence deduced from its coding cDNA, demonstrated its profilin nature. An unexpectedly high reactivity to Cit s 2 was found in vivo (78% of positive SPT responses) and in vitro (87% of sera from orange allergic patients had specific IgE to Cit s 2). The purified allergen inhibited around 50% of the IgE binding to an orange pulp extract.

CONCLUSION

Orange profilin Cit s 2, unlike other plant food profilins, is a major and highly prevalent allergen.

Authors+Show Affiliations

Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16197476

Citation

López-Torrejón, G, et al. "Isolation, Cloning and Allergenic Reactivity of Natural Profilin Cit S 2, a Major Orange Allergen." Allergy, vol. 60, no. 11, 2005, pp. 1424-9.
López-Torrejón G, Ibáñez MD, Ahrazem O, et al. Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen. Allergy. 2005;60(11):1424-9.
López-Torrejón, G., Ibáñez, M. D., Ahrazem, O., Sánchez-Monge, R., Sastre, J., Lombardero, M., ... Salcedo, G. (2005). Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen. Allergy, 60(11), pp. 1424-9.
López-Torrejón G, et al. Isolation, Cloning and Allergenic Reactivity of Natural Profilin Cit S 2, a Major Orange Allergen. Allergy. 2005;60(11):1424-9. PubMed PMID: 16197476.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation, cloning and allergenic reactivity of natural profilin Cit s 2, a major orange allergen. AU - López-Torrejón,G, AU - Ibáñez,M D, AU - Ahrazem,O, AU - Sánchez-Monge,R, AU - Sastre,J, AU - Lombardero,M, AU - Barber,D, AU - Salcedo,G, PY - 2005/10/4/pubmed PY - 2006/3/24/medline PY - 2005/10/4/entrez SP - 1424 EP - 9 JF - Allergy JO - Allergy VL - 60 IS - 11 N2 - BACKGROUND: Orange is among the most widely consumed fruits, and among the plant food sources causing allergic reactions according to popular perception. However, its relevant allergenic components are virtually unknown. Profilin is a well-defined minor plant panallergen, showing prevalences around 30% in fruits and vegetables. METHODS: Twenty-three orange-allergic patients were studied. Natural orange profilin, named Cit s 2, was purified by affinity chromatography and characterized by N-terminal amino acid sequencing, matrix-assisted laser desorption/ionization mass spectrometry analysis and isolation of its coding cDNA. Reactivity to Cit s 2 was analyzed in vivo by skin prick tests (SPT) and in vitro by IgE immunodetection, specific IgE determination in individual sera and enzyme-linked immunosorbent assay-inhibition assays. RESULTS: The N-terminal amino acid sequence and molecular mass of natural Cit s 2, both fully in agreement with the complete amino acid sequence deduced from its coding cDNA, demonstrated its profilin nature. An unexpectedly high reactivity to Cit s 2 was found in vivo (78% of positive SPT responses) and in vitro (87% of sera from orange allergic patients had specific IgE to Cit s 2). The purified allergen inhibited around 50% of the IgE binding to an orange pulp extract. CONCLUSION: Orange profilin Cit s 2, unlike other plant food profilins, is a major and highly prevalent allergen. SN - 0105-4538 UR - https://www.unboundmedicine.com/medline/citation/16197476/Isolation_cloning_and_allergenic_reactivity_of_natural_profilin_Cit_s_2_a_major_orange_allergen_ L2 - https://doi.org/10.1111/j.1398-9995.2005.00903.x DB - PRIME DP - Unbound Medicine ER -