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Expression of phosphorylated c-Jun N-terminal protein kinase (JNK) in experimental glaucoma in rats.
Exp Eye Res. 2006 Apr; 82(4):576-82.EE

Abstract

To examine the expression of phosphorylated c-Jun N-terminal kinase (JNK) in cells in the retinal ganglion cell layer of glaucoma, intraocular pressure (IOP) of adult Wistar rats was elevated unilaterally by repeated trabecular argon laser photocoagulation 5 days after intracameral injection of India ink. Animals were euthanized after 3 days, and 1, 2, and 5 weeks of IOP elevation. Immunohistochemistry with specific antibodies against phosphorylated JNK was performed on retinas. Retrograde labeling using Fluorogold and fluorescence immunohistochemistry was performed on retinas 5 weeks after IOP elevation. TdT-mediated biotin-dUTP nick end labeling (TUNEL) was performed on the retinal sections to determine the rate of cell death. There was increased IOP (52.3%) from 3 days to 5 weeks after repeated trabecular laser photocoagulation. Mean number of TUNEL-positive cells in the retinal ganglion cell layer of eyes with experimental glaucoma was 0.43, 0.36, 0.57, and 0.19 per retinal section at 3 days, and 1, 2 and 5 weeks, respectively. No TUNEL-positive cells were noted in controls. In parallel to TUNEL, significantly increased numbers of phosphorylated JNK-labeled cells in the retinal ganglion cell layer were noted at 3 days (9.95 versus 4.15; P=0.005), 1 week (7.65 versus 4.00; P=0.006), 2 weeks (9.13 versus 4.48; P=0.032), and 5 weeks (8.06 versus 4.96; P=0.017) of IOP elevation when compared with contralateral control eyes. Fluorogold labeled RGCs were co-localized with increased phosphorylated JNK immunoreactivity. Some TUNEL-positive cells were phosphorylated JNK immuno-positive. Phosphorylation of JNK occurs in experimental glaucoma and may play a role in retinal ganglion cell death.

Authors+Show Affiliations

Department of Ophthalmology, Jules Stein Eye Institute, David Geffen School of Medicine at University of California Los Angeles, Room B-146, 100 Stein Plaza, Los Angeles, CA 90095-7000, USA. kwong@jsei.ucla.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16197943

Citation

Kwong, Jacky M K., and Joseph Caprioli. "Expression of Phosphorylated c-Jun N-terminal Protein Kinase (JNK) in Experimental Glaucoma in Rats." Experimental Eye Research, vol. 82, no. 4, 2006, pp. 576-82.
Kwong JM, Caprioli J. Expression of phosphorylated c-Jun N-terminal protein kinase (JNK) in experimental glaucoma in rats. Exp Eye Res. 2006;82(4):576-82.
Kwong, J. M., & Caprioli, J. (2006). Expression of phosphorylated c-Jun N-terminal protein kinase (JNK) in experimental glaucoma in rats. Experimental Eye Research, 82(4), 576-82.
Kwong JM, Caprioli J. Expression of Phosphorylated c-Jun N-terminal Protein Kinase (JNK) in Experimental Glaucoma in Rats. Exp Eye Res. 2006;82(4):576-82. PubMed PMID: 16197943.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of phosphorylated c-Jun N-terminal protein kinase (JNK) in experimental glaucoma in rats. AU - Kwong,Jacky M K, AU - Caprioli,Joseph, Y1 - 2005/09/28/ PY - 2004/12/29/received PY - 2005/08/06/revised PY - 2005/08/17/accepted PY - 2005/10/4/pubmed PY - 2006/7/6/medline PY - 2005/10/4/entrez SP - 576 EP - 82 JF - Experimental eye research JO - Exp Eye Res VL - 82 IS - 4 N2 - To examine the expression of phosphorylated c-Jun N-terminal kinase (JNK) in cells in the retinal ganglion cell layer of glaucoma, intraocular pressure (IOP) of adult Wistar rats was elevated unilaterally by repeated trabecular argon laser photocoagulation 5 days after intracameral injection of India ink. Animals were euthanized after 3 days, and 1, 2, and 5 weeks of IOP elevation. Immunohistochemistry with specific antibodies against phosphorylated JNK was performed on retinas. Retrograde labeling using Fluorogold and fluorescence immunohistochemistry was performed on retinas 5 weeks after IOP elevation. TdT-mediated biotin-dUTP nick end labeling (TUNEL) was performed on the retinal sections to determine the rate of cell death. There was increased IOP (52.3%) from 3 days to 5 weeks after repeated trabecular laser photocoagulation. Mean number of TUNEL-positive cells in the retinal ganglion cell layer of eyes with experimental glaucoma was 0.43, 0.36, 0.57, and 0.19 per retinal section at 3 days, and 1, 2 and 5 weeks, respectively. No TUNEL-positive cells were noted in controls. In parallel to TUNEL, significantly increased numbers of phosphorylated JNK-labeled cells in the retinal ganglion cell layer were noted at 3 days (9.95 versus 4.15; P=0.005), 1 week (7.65 versus 4.00; P=0.006), 2 weeks (9.13 versus 4.48; P=0.032), and 5 weeks (8.06 versus 4.96; P=0.017) of IOP elevation when compared with contralateral control eyes. Fluorogold labeled RGCs were co-localized with increased phosphorylated JNK immunoreactivity. Some TUNEL-positive cells were phosphorylated JNK immuno-positive. Phosphorylation of JNK occurs in experimental glaucoma and may play a role in retinal ganglion cell death. SN - 0014-4835 UR - https://www.unboundmedicine.com/medline/citation/16197943/Expression_of_phosphorylated_c_Jun_N_terminal_protein_kinase__JNK__in_experimental_glaucoma_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(05)00258-7 DB - PRIME DP - Unbound Medicine ER -