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Speciation of cationic selenium compounds in Brassica juncea leaves by strong cation-exchange chromatography with inductively coupled plasma mass spectrometry.
J Chromatogr A. 2005 Dec 30; 1100(2):153-9.JC

Abstract

Strong cation-exchange chromatography (SCX-HPLC) was used in conjunction with inductively coupled plasma mass spectrometry (ICP-MS) to investigate cationic selenium species present in leaf extract of wild-type Brassica juncea supplemented with selenite. Total amount of Se accumulated by the leaves was found to be 352 microg g(-1). Cation-exchange solid-phase extraction (SCX-SPE) was used to pre-concentrate the cationic species present in the leaf extract. Methylselenomethionine (MeSeMet) and dimethylselenoniumproprionate (DMSeP) were synthesized and characterized by electrospray quadrupole time-of-flight MS (ESI-QTOF-MS). Laboratory synthesized and commercially available standards were used in chromatographic studies to identify the Se species in the leaf extract through retention time comparisons and standard addition method. Major cationic selenium species identified in the present study were MeSeMet and methylselenocysteine (MeSeCys) while selenomethionine (SeMet) was found in minor quantities.

Authors+Show Affiliations

Yathavakilla SV
Department of Chemistry, University of Cincinnati, OH 45221-0172, USA.
Shah M
No affiliation info available
Mounicou S
No affiliation info available
Caruso JA
No affiliation info available

MeSH

Chemical FractionationChromatography, Ion ExchangeMustard PlantPlant LeavesSeleniumSelenium CompoundsSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16198358

Citation

Yathavakilla, Santha V Ketavarapu, et al. "Speciation of Cationic Selenium Compounds in Brassica Juncea Leaves By Strong Cation-exchange Chromatography With Inductively Coupled Plasma Mass Spectrometry." Journal of Chromatography. A, vol. 1100, no. 2, 2005, pp. 153-9.
Yathavakilla SV, Shah M, Mounicou S, et al. Speciation of cationic selenium compounds in Brassica juncea leaves by strong cation-exchange chromatography with inductively coupled plasma mass spectrometry. J Chromatogr A. 2005;1100(2):153-9.
Yathavakilla, S. V., Shah, M., Mounicou, S., & Caruso, J. A. (2005). Speciation of cationic selenium compounds in Brassica juncea leaves by strong cation-exchange chromatography with inductively coupled plasma mass spectrometry. Journal of Chromatography. A, 1100(2), 153-9.
Yathavakilla SV, et al. Speciation of Cationic Selenium Compounds in Brassica Juncea Leaves By Strong Cation-exchange Chromatography With Inductively Coupled Plasma Mass Spectrometry. J Chromatogr A. 2005 Dec 30;1100(2):153-9. PubMed PMID: 16198358.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Speciation of cationic selenium compounds in Brassica juncea leaves by strong cation-exchange chromatography with inductively coupled plasma mass spectrometry. AU - Yathavakilla,Santha V Ketavarapu, AU - Shah,Monika, AU - Mounicou,Sandra, AU - Caruso,Joseph A, Y1 - 2005/09/28/ PY - 2005/04/25/received PY - 2005/08/31/revised PY - 2005/09/12/accepted PY - 2005/10/4/pubmed PY - 2006/2/24/medline PY - 2005/10/4/entrez SP - 153 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1100 IS - 2 N2 - Strong cation-exchange chromatography (SCX-HPLC) was used in conjunction with inductively coupled plasma mass spectrometry (ICP-MS) to investigate cationic selenium species present in leaf extract of wild-type Brassica juncea supplemented with selenite. Total amount of Se accumulated by the leaves was found to be 352 microg g(-1). Cation-exchange solid-phase extraction (SCX-SPE) was used to pre-concentrate the cationic species present in the leaf extract. Methylselenomethionine (MeSeMet) and dimethylselenoniumproprionate (DMSeP) were synthesized and characterized by electrospray quadrupole time-of-flight MS (ESI-QTOF-MS). Laboratory synthesized and commercially available standards were used in chromatographic studies to identify the Se species in the leaf extract through retention time comparisons and standard addition method. Major cationic selenium species identified in the present study were MeSeMet and methylselenocysteine (MeSeCys) while selenomethionine (SeMet) was found in minor quantities. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16198358/Speciation_of_cationic_selenium_compounds_in_Brassica_juncea_leaves_by_strong_cation_exchange_chromatography_with_inductively_coupled_plasma_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -