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Determination and analyses of the N-termini of oil-body proteins, steroleosin, caleosin and oleosin.
Plant Physiol Biochem. 2005 Aug; 43(8):770-6.PP

Abstract

Seed oil bodies comprise a triacylglycerol matrix shielded by a monolayer of phospholipids and proteins. These surface proteins include an abundant structural protein, oleosin, and at least two minor protein classes termed caleosin and steroleosin. Two steroleosin isoforms (41 and 39 kDa), one caleosin (27 kDa), and two oleosin isoforms (17 and 15 kDa) have been identified in oil bodies isolated from sesame seeds. The signal peptides responsible for targeting of these proteins to oil bodies have not been experimentally determined. Hydropathy analyses indicate that the hydrophobic domain putatively responsible for oil-body anchoring is located in the N-terminal region of steroleosin, but in the central region of caleosin or oleosin. Direct amino acid sequencing showed that both steroleosin isoforms possessed a free methionine residue at their N-termini while caleosin and oleosin isoforms were N-terminally blocked. Mass spectrometry analyses revealed that N-termini of both caleosin and 17 kDa oleosin were acetylated after the removal of the first methionine. In addition, deamidation was observed at a glutamine residue in the N-terminal region of 17 kDa oleosin.

Authors+Show Affiliations

Graduate Institute of Biotechnology, National Chung-Hsing University, 40227 Taichung, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16198588

Citation

Lin, Li-Jen, et al. "Determination and Analyses of the N-termini of Oil-body Proteins, Steroleosin, Caleosin and Oleosin." Plant Physiology and Biochemistry : PPB, vol. 43, no. 8, 2005, pp. 770-6.
Lin LJ, Liao PC, Yang HH, et al. Determination and analyses of the N-termini of oil-body proteins, steroleosin, caleosin and oleosin. Plant Physiol Biochem. 2005;43(8):770-6.
Lin, L. J., Liao, P. C., Yang, H. H., & Tzen, J. T. (2005). Determination and analyses of the N-termini of oil-body proteins, steroleosin, caleosin and oleosin. Plant Physiology and Biochemistry : PPB, 43(8), 770-6.
Lin LJ, et al. Determination and Analyses of the N-termini of Oil-body Proteins, Steroleosin, Caleosin and Oleosin. Plant Physiol Biochem. 2005;43(8):770-6. PubMed PMID: 16198588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination and analyses of the N-termini of oil-body proteins, steroleosin, caleosin and oleosin. AU - Lin,Li-Jen, AU - Liao,Pao-Chi, AU - Yang,Hsueh-Hui, AU - Tzen,Jason T C, Y1 - 2005/08/31/ PY - 2005/05/16/received PY - 2005/06/07/revised PY - 2005/07/26/accepted PY - 2005/10/4/pubmed PY - 2006/5/25/medline PY - 2005/10/4/entrez SP - 770 EP - 6 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 43 IS - 8 N2 - Seed oil bodies comprise a triacylglycerol matrix shielded by a monolayer of phospholipids and proteins. These surface proteins include an abundant structural protein, oleosin, and at least two minor protein classes termed caleosin and steroleosin. Two steroleosin isoforms (41 and 39 kDa), one caleosin (27 kDa), and two oleosin isoforms (17 and 15 kDa) have been identified in oil bodies isolated from sesame seeds. The signal peptides responsible for targeting of these proteins to oil bodies have not been experimentally determined. Hydropathy analyses indicate that the hydrophobic domain putatively responsible for oil-body anchoring is located in the N-terminal region of steroleosin, but in the central region of caleosin or oleosin. Direct amino acid sequencing showed that both steroleosin isoforms possessed a free methionine residue at their N-termini while caleosin and oleosin isoforms were N-terminally blocked. Mass spectrometry analyses revealed that N-termini of both caleosin and 17 kDa oleosin were acetylated after the removal of the first methionine. In addition, deamidation was observed at a glutamine residue in the N-terminal region of 17 kDa oleosin. SN - 0981-9428 UR - https://www.unboundmedicine.com/medline/citation/16198588/Determination_and_analyses_of_the_N_termini_of_oil_body_proteins_steroleosin_caleosin_and_oleosin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(05)00177-4 DB - PRIME DP - Unbound Medicine ER -