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Preparative isolation and separation of a novel and two known flavonoids from Patrinia villosa Juss by high-speed counter-current chromatography.
J Chromatogr A. 2005 Oct 28; 1092(2):235-40.JC

Abstract

Preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and separation three flavonoids including bolusanthol B, a novel compound named 5,7,2',6'-tetrahydroxy-6,8-di(gamma,gamma-dimethylallyl) flavanone and tetrapterol I from Patrinia villosa Juss using two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at the volume ratio of 10:11:11:8 (v/v). A total of 25.4 mg bolusanthol B, 52.5 mg 5,7,2',6'-tetrahydroxy-6,8-di(gamma,gamma-dimethylallyl) flavanone and 50.2 mg tetrapterol I were obtained from 250 mg crude extract with purities of 96.8%, 99.2% and 99.3%, respectively determined by HPLC in one single operation and less than 5 h. The structure identification was performed by UV, IR, MS, 1H NMR, 13C NMR and 2D NMR. Among then, bolusanthol B and tetrapterol I were obtained from the plant of Patrinia genius for the first time, and 5,7,2',6'-tetrahydroxy-6,8-di(gamma,gamma-dimethylallyl) flavanone was a novel prenylated flavonoid and discovered from nature for the first time.

Authors+Show Affiliations

Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16199231

Citation

Peng, Jinyong, et al. "Preparative Isolation and Separation of a Novel and Two Known Flavonoids From Patrinia Villosa Juss By High-speed Counter-current Chromatography." Journal of Chromatography. A, vol. 1092, no. 2, 2005, pp. 235-40.
Peng J, Yang G, Fan G, et al. Preparative isolation and separation of a novel and two known flavonoids from Patrinia villosa Juss by high-speed counter-current chromatography. J Chromatogr A. 2005;1092(2):235-40.
Peng, J., Yang, G., Fan, G., & Wu, Y. (2005). Preparative isolation and separation of a novel and two known flavonoids from Patrinia villosa Juss by high-speed counter-current chromatography. Journal of Chromatography. A, 1092(2), 235-40.
Peng J, et al. Preparative Isolation and Separation of a Novel and Two Known Flavonoids From Patrinia Villosa Juss By High-speed Counter-current Chromatography. J Chromatogr A. 2005 Oct 28;1092(2):235-40. PubMed PMID: 16199231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparative isolation and separation of a novel and two known flavonoids from Patrinia villosa Juss by high-speed counter-current chromatography. AU - Peng,Jinyong, AU - Yang,Genjin, AU - Fan,Guorong, AU - Wu,Yutian, PY - 2005/05/21/received PY - 2005/07/08/revised PY - 2005/07/11/accepted PY - 2005/10/4/pubmed PY - 2005/12/13/medline PY - 2005/10/4/entrez SP - 235 EP - 40 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1092 IS - 2 N2 - Preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and separation three flavonoids including bolusanthol B, a novel compound named 5,7,2',6'-tetrahydroxy-6,8-di(gamma,gamma-dimethylallyl) flavanone and tetrapterol I from Patrinia villosa Juss using two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at the volume ratio of 10:11:11:8 (v/v). A total of 25.4 mg bolusanthol B, 52.5 mg 5,7,2',6'-tetrahydroxy-6,8-di(gamma,gamma-dimethylallyl) flavanone and 50.2 mg tetrapterol I were obtained from 250 mg crude extract with purities of 96.8%, 99.2% and 99.3%, respectively determined by HPLC in one single operation and less than 5 h. The structure identification was performed by UV, IR, MS, 1H NMR, 13C NMR and 2D NMR. Among then, bolusanthol B and tetrapterol I were obtained from the plant of Patrinia genius for the first time, and 5,7,2',6'-tetrahydroxy-6,8-di(gamma,gamma-dimethylallyl) flavanone was a novel prenylated flavonoid and discovered from nature for the first time. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16199231/Preparative_isolation_and_separation_of_a_novel_and_two_known_flavonoids_from_Patrinia_villosa_Juss_by_high_speed_counter_current_chromatography_ DB - PRIME DP - Unbound Medicine ER -
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