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Endothelial cell-specific expression of Cre recombinase in transgenic mice.
Yi Chuan Xue Bao. 2005 Sep; 32(9):909-15.YC

Abstract

Endothelial cells participate in angiogenesis, vascular homeostasis, thrombosis, inflammation and vascular wall remodeling. To study the function of genes in endothelial cells using Cre-loxP system, we generated Tie2-Cre transgenic mice, in which expression of Cre recombinase is driven by Tie2 promoter. Total six founder mice carrying the Tie2-Cre transgene were identified by genomic PCR and Southern blot. The integration efficiency is 11%. In order to test the excision activity and tissue distribution of the Cre recombinase, the Tie2-Cre transgenic line was crossed with the mouse strain carrying the Smad4 conditional alleles (Smad4(Co/Co)) or the reporter line ROSA26. PCR of multiple tissue DNA from Tie2-Cre; Smad4(Co/+) mice revealed the Cre activity in all tissues containing endothelial cells. We detected pan-endothelial expression of the Cre transgene in Tie2-Cre; ROSA26 double transgenic embryos by lacZ staining. Therefore, this mouse line may serve as a valuable tool for endothelial cell lineage analyses and conditional gene ablation in endothelial cells.

Authors+Show Affiliations

Genetic Laboratory of Development and Diseases, Institute of Biotechnology, Beijing 100071, China. dragonliwen@sohu.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16201233

Citation

Li, Wen-Long, et al. "Endothelial Cell-specific Expression of Cre Recombinase in Transgenic Mice." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 32, no. 9, 2005, pp. 909-15.
Li WL, Cheng X, Tan XH, et al. Endothelial cell-specific expression of Cre recombinase in transgenic mice. Yi Chuan Xue Bao. 2005;32(9):909-15.
Li, W. L., Cheng, X., Tan, X. H., Zhang, J. S., Sun, Y. S., Chen, L., & Yang, X. (2005). Endothelial cell-specific expression of Cre recombinase in transgenic mice. Yi Chuan Xue Bao = Acta Genetica Sinica, 32(9), 909-15.
Li WL, et al. Endothelial Cell-specific Expression of Cre Recombinase in Transgenic Mice. Yi Chuan Xue Bao. 2005;32(9):909-15. PubMed PMID: 16201233.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endothelial cell-specific expression of Cre recombinase in transgenic mice. AU - Li,Wen-Long, AU - Cheng,Xuan, AU - Tan,Xiao-Hong, AU - Zhang,Ji-Shuai, AU - Sun,Yan-Song, AU - Chen,Lin, AU - Yang,Xiao, PY - 2005/10/6/pubmed PY - 2008/8/19/medline PY - 2005/10/6/entrez SP - 909 EP - 15 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 32 IS - 9 N2 - Endothelial cells participate in angiogenesis, vascular homeostasis, thrombosis, inflammation and vascular wall remodeling. To study the function of genes in endothelial cells using Cre-loxP system, we generated Tie2-Cre transgenic mice, in which expression of Cre recombinase is driven by Tie2 promoter. Total six founder mice carrying the Tie2-Cre transgene were identified by genomic PCR and Southern blot. The integration efficiency is 11%. In order to test the excision activity and tissue distribution of the Cre recombinase, the Tie2-Cre transgenic line was crossed with the mouse strain carrying the Smad4 conditional alleles (Smad4(Co/Co)) or the reporter line ROSA26. PCR of multiple tissue DNA from Tie2-Cre; Smad4(Co/+) mice revealed the Cre activity in all tissues containing endothelial cells. We detected pan-endothelial expression of the Cre transgene in Tie2-Cre; ROSA26 double transgenic embryos by lacZ staining. Therefore, this mouse line may serve as a valuable tool for endothelial cell lineage analyses and conditional gene ablation in endothelial cells. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/16201233/Endothelial_cell_specific_expression_of_Cre_recombinase_in_transgenic_mice_ L2 - http://www.komp.org/ncbi.php?PMID=16201233 DB - PRIME DP - Unbound Medicine ER -