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Towards an expanded linkage map and exploration on co-dominant scoring of AFLPs in maize.
Yi Chuan Xue Bao. 2005 Sep; 32(9):960-8.YC

Abstract

Simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs) have become the most important markers for molecular mapping. Primarily based on restriction fragment length polymorphism (RFLP) markers, extensive linkage maps of maize had been developed. To construct a near-saturated genetic linkage map, an expanded maize genetic linkage map was constructed using a population of 234 F2 individuals derived from a cross of X178 and B73 base on an essential SSR framework map of maize. The level of polymorphisms and genetic properties of SSR and AFLP markers were characterized. A total of 249 markers consisting of 130 SSRs and 119 AFLPs have been landed on 10 chromosomes of maize. The 249-locus map spanned 1 659.3 centi-morgans (cM) and had a mean density of 6.66 cM. This mapping population and related information should connect further research involving analysis of quantitative trait loci, comparative genomics, and heterosis. Moreover, in many studies, AFLPs were analyzed on the basis of the presence or absence of a band on the electrophoresis gels. A new method based on double polymorphic bands of co-dominant scoring of AFLPs was explored according to the similarity of loci amplified from AFLP enzyme combination.

Authors+Show Affiliations

Hao ZF
Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081, China. zf.hao@sohu.com
Li XH
No affiliation info available
Zhang SH
No affiliation info available

MeSH

Amplified Fragment Length Polymorphism AnalysisChromosome MappingChromosomes, PlantDNA, PlantGenes, DominantHybridization, GeneticMicrosatellite RepeatsPolymorphism, GeneticQuantitative Trait LociZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16201241

Citation

Hao, Zhuan-Fang, et al. "Towards an Expanded Linkage Map and Exploration On Co-dominant Scoring of AFLPs in Maize." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 32, no. 9, 2005, pp. 960-8.
Hao ZF, Li XH, Zhang SH. Towards an expanded linkage map and exploration on co-dominant scoring of AFLPs in maize. Yi Chuan Xue Bao. 2005;32(9):960-8.
Hao, Z. F., Li, X. H., & Zhang, S. H. (2005). Towards an expanded linkage map and exploration on co-dominant scoring of AFLPs in maize. Yi Chuan Xue Bao = Acta Genetica Sinica, 32(9), 960-8.
Hao ZF, Li XH, Zhang SH. Towards an Expanded Linkage Map and Exploration On Co-dominant Scoring of AFLPs in Maize. Yi Chuan Xue Bao. 2005;32(9):960-8. PubMed PMID: 16201241.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Towards an expanded linkage map and exploration on co-dominant scoring of AFLPs in maize. AU - Hao,Zhuan-Fang, AU - Li,Xin-Hai, AU - Zhang,Shi-Huang, PY - 2005/10/6/pubmed PY - 2008/8/19/medline PY - 2005/10/6/entrez SP - 960 EP - 8 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 32 IS - 9 N2 - Simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs) have become the most important markers for molecular mapping. Primarily based on restriction fragment length polymorphism (RFLP) markers, extensive linkage maps of maize had been developed. To construct a near-saturated genetic linkage map, an expanded maize genetic linkage map was constructed using a population of 234 F2 individuals derived from a cross of X178 and B73 base on an essential SSR framework map of maize. The level of polymorphisms and genetic properties of SSR and AFLP markers were characterized. A total of 249 markers consisting of 130 SSRs and 119 AFLPs have been landed on 10 chromosomes of maize. The 249-locus map spanned 1 659.3 centi-morgans (cM) and had a mean density of 6.66 cM. This mapping population and related information should connect further research involving analysis of quantitative trait loci, comparative genomics, and heterosis. Moreover, in many studies, AFLPs were analyzed on the basis of the presence or absence of a band on the electrophoresis gels. A new method based on double polymorphic bands of co-dominant scoring of AFLPs was explored according to the similarity of loci amplified from AFLP enzyme combination. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/16201241/Towards_an_expanded_linkage_map_and_exploration_on_co_dominant_scoring_of_AFLPs_in_maize_ DB - PRIME DP - Unbound Medicine ER -