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Oxalomalate, a competitive inhibitor of NADP+-dependent isocitrate dehydrogenase, regulates heat shock-induced apoptosis.
Biochem Biophys Res Commun. 2005 Nov 18; 337(2):685-91.BB

Abstract

Heat shock may increase oxidative stress due to increased production of reactive oxygen species and/or the promotion of cellular oxidation events. Recently, we demonstrated that the control of cytosolic and mitochondrial redox balance and the cellular defense against oxidative damage is one of the primary functions of NADP(+)-dependent isocitrate dehydrogenase (ICDH) by supplying NADPH for antioxidant systems. The protective role of ICDH against heat shock-induced apoptosis in U937 cells was investigated in the control and the cells pre-treated with oxalomalate, a competitive inhibitor of ICDH. Upon exposure to heat shock, there was a distinct difference between the control cells and the cells pre-treated with 3mM oxalomalate for 3h in regard to apoptotic parameters, cellular redox status, and mitochondrial function. The oxalomalate pre-treated cells showed significant enhancement of apoptotic features such as activation of caspase-3, up-regulation of Bax, and down-regulation of Bcl-2 compared to the control cells upon exposure to heat shock. This study indicates that ICDH may play an important role in regulating the apoptosis induced by heat shock presumably through maintaining the cellular redox status.

Authors+Show Affiliations

School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu, Republic of Korea.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16202391

Citation

Kim, Hyun Jeong, and Jeen-Woo Park. "Oxalomalate, a Competitive Inhibitor of NADP+-dependent Isocitrate Dehydrogenase, Regulates Heat Shock-induced Apoptosis." Biochemical and Biophysical Research Communications, vol. 337, no. 2, 2005, pp. 685-91.
Kim HJ, Park JW. Oxalomalate, a competitive inhibitor of NADP+-dependent isocitrate dehydrogenase, regulates heat shock-induced apoptosis. Biochem Biophys Res Commun. 2005;337(2):685-91.
Kim, H. J., & Park, J. W. (2005). Oxalomalate, a competitive inhibitor of NADP+-dependent isocitrate dehydrogenase, regulates heat shock-induced apoptosis. Biochemical and Biophysical Research Communications, 337(2), 685-91.
Kim HJ, Park JW. Oxalomalate, a Competitive Inhibitor of NADP+-dependent Isocitrate Dehydrogenase, Regulates Heat Shock-induced Apoptosis. Biochem Biophys Res Commun. 2005 Nov 18;337(2):685-91. PubMed PMID: 16202391.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxalomalate, a competitive inhibitor of NADP+-dependent isocitrate dehydrogenase, regulates heat shock-induced apoptosis. AU - Kim,Hyun Jeong, AU - Park,Jeen-Woo, Y1 - 2005/09/26/ PY - 2005/09/14/received PY - 2005/09/16/accepted PY - 2005/10/6/pubmed PY - 2005/12/22/medline PY - 2005/10/6/entrez SP - 685 EP - 91 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 337 IS - 2 N2 - Heat shock may increase oxidative stress due to increased production of reactive oxygen species and/or the promotion of cellular oxidation events. Recently, we demonstrated that the control of cytosolic and mitochondrial redox balance and the cellular defense against oxidative damage is one of the primary functions of NADP(+)-dependent isocitrate dehydrogenase (ICDH) by supplying NADPH for antioxidant systems. The protective role of ICDH against heat shock-induced apoptosis in U937 cells was investigated in the control and the cells pre-treated with oxalomalate, a competitive inhibitor of ICDH. Upon exposure to heat shock, there was a distinct difference between the control cells and the cells pre-treated with 3mM oxalomalate for 3h in regard to apoptotic parameters, cellular redox status, and mitochondrial function. The oxalomalate pre-treated cells showed significant enhancement of apoptotic features such as activation of caspase-3, up-regulation of Bax, and down-regulation of Bcl-2 compared to the control cells upon exposure to heat shock. This study indicates that ICDH may play an important role in regulating the apoptosis induced by heat shock presumably through maintaining the cellular redox status. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/16202391/Oxalomalate_a_competitive_inhibitor_of_NADP+_dependent_isocitrate_dehydrogenase_regulates_heat_shock_induced_apoptosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(05)02123-6 DB - PRIME DP - Unbound Medicine ER -