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Na(+), K(+)-ATPase content in skeletal muscle of dogs with pituitary-dependent hyperadrenocorticism.
Domest Anim Endocrinol. 2006 May; 30(4):320-32.DA

Abstract

Several hormones regulate Na(+), K(+)-ATPase content in the muscle cell membrane, which is essential for maintaining muscle cell excitability. Chronic glucocorticoid excess is associated with muscle weakness and reduced endurance. We hypothesized that chronic glucocorticoid excess affects Na(+), K(+)-ATPase content in canine skeletal muscle, and contributes to reduced endurance and muscle weakness associated with pituitary-dependent hyperadrenocorticism (PDH) in dogs. Therefore, Na(+), K(+)-ATPase content in skeletal muscle was evaluated before and after hypophysectomy and hormone replacement (cortisone and l-thyroxin) in dogs with PDH (n=13), and in healthy controls (n=6). In addition, baseline and exercise-induced changes in plasma electrolyte concentrations and acid-base balance were evaluated before and after hypophysectomy in dogs with PDH. Na(+), K(+)-ATPase content of gluteal muscle in dogs with PDH was significantly lower than in control dogs (201+/-13pmol/g versus 260+/-8pmol/g wet weight; P<0.01). Similar differences were found in palatine muscle. After hypophysectomy and on hormone replacement, Na(+), K(+)-ATPase was increased (234+/-7pmol/g wet weight). Both plasma pH and base excess in dogs with PDH (7.44+/-0.01; 1.7+/-0.6mmol/l, respectively) were significantly higher (P<0.05) than after hypophysectomy and hormone replacement (7.41+/-0.01; -0.2+/-0.4mmol/l, respectively). Exercise induced respiratory alkalosis, but did not result in hyperkalemia in dogs with PDH. In conclusion, chronic glucocorticoid excess in dogs with PDH is associated with decreased Na(+), K(+)-ATPase content in skeletal muscle. This may contribute to reduce endurance in canine PDH, although dogs with PDH did not exhibit exercise-induced hyperkalemia. Na(+), K(+)-ATPase content normalized to values statistically not different from healthy controls after hypophysectomy and hormone replacement.

Authors+Show Affiliations

Schotanus BA
Department of Pathobiology, Division of Anatomy and Physiology, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.158, 3508 TD Utrecht, The Netherlands.
Meij BP
No affiliation info available
Vos IH
No affiliation info available
Kooistra HS
No affiliation info available
Everts ME
No affiliation info available

MeSH

Adrenocortical HyperfunctionAdrenocorticotropic HormoneAnimalsBloodDog DiseasesDogsFemaleGlucocorticoidsGrowth HormoneHormone Replacement TherapyHydrocortisoneHydrogen-Ion ConcentrationHypophysectomyInsulin-Like Growth Factor IMaleMuscle, SkeletalOuabainPhysical EndurancePhysical ExertionPituitary NeoplasmsSodium-Potassium-Exchanging ATPaseThyrotropinThyroxineTritium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16202554

Citation

Schotanus, B A., et al. "Na(+), K(+)-ATPase Content in Skeletal Muscle of Dogs With Pituitary-dependent Hyperadrenocorticism." Domestic Animal Endocrinology, vol. 30, no. 4, 2006, pp. 320-32.
Schotanus BA, Meij BP, Vos IH, et al. Na(+), K(+)-ATPase content in skeletal muscle of dogs with pituitary-dependent hyperadrenocorticism. Domest Anim Endocrinol. 2006;30(4):320-32.
Schotanus, B. A., Meij, B. P., Vos, I. H., Kooistra, H. S., & Everts, M. E. (2006). Na(+), K(+)-ATPase content in skeletal muscle of dogs with pituitary-dependent hyperadrenocorticism. Domestic Animal Endocrinology, 30(4), 320-32.
Schotanus BA, et al. Na(+), K(+)-ATPase Content in Skeletal Muscle of Dogs With Pituitary-dependent Hyperadrenocorticism. Domest Anim Endocrinol. 2006;30(4):320-32. PubMed PMID: 16202554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Na(+), K(+)-ATPase content in skeletal muscle of dogs with pituitary-dependent hyperadrenocorticism. AU - Schotanus,B A, AU - Meij,B P, AU - Vos,I H C, AU - Kooistra,H S, AU - Everts,M E, Y1 - 2005/09/15/ PY - 2005/06/17/received PY - 2005/08/25/revised PY - 2005/08/25/accepted PY - 2005/10/6/pubmed PY - 2006/6/10/medline PY - 2005/10/6/entrez SP - 320 EP - 32 JF - Domestic animal endocrinology JO - Domest Anim Endocrinol VL - 30 IS - 4 N2 - Several hormones regulate Na(+), K(+)-ATPase content in the muscle cell membrane, which is essential for maintaining muscle cell excitability. Chronic glucocorticoid excess is associated with muscle weakness and reduced endurance. We hypothesized that chronic glucocorticoid excess affects Na(+), K(+)-ATPase content in canine skeletal muscle, and contributes to reduced endurance and muscle weakness associated with pituitary-dependent hyperadrenocorticism (PDH) in dogs. Therefore, Na(+), K(+)-ATPase content in skeletal muscle was evaluated before and after hypophysectomy and hormone replacement (cortisone and l-thyroxin) in dogs with PDH (n=13), and in healthy controls (n=6). In addition, baseline and exercise-induced changes in plasma electrolyte concentrations and acid-base balance were evaluated before and after hypophysectomy in dogs with PDH. Na(+), K(+)-ATPase content of gluteal muscle in dogs with PDH was significantly lower than in control dogs (201+/-13pmol/g versus 260+/-8pmol/g wet weight; P<0.01). Similar differences were found in palatine muscle. After hypophysectomy and on hormone replacement, Na(+), K(+)-ATPase was increased (234+/-7pmol/g wet weight). Both plasma pH and base excess in dogs with PDH (7.44+/-0.01; 1.7+/-0.6mmol/l, respectively) were significantly higher (P<0.05) than after hypophysectomy and hormone replacement (7.41+/-0.01; -0.2+/-0.4mmol/l, respectively). Exercise induced respiratory alkalosis, but did not result in hyperkalemia in dogs with PDH. In conclusion, chronic glucocorticoid excess in dogs with PDH is associated with decreased Na(+), K(+)-ATPase content in skeletal muscle. This may contribute to reduce endurance in canine PDH, although dogs with PDH did not exhibit exercise-induced hyperkalemia. Na(+), K(+)-ATPase content normalized to values statistically not different from healthy controls after hypophysectomy and hormone replacement. SN - 0739-7240 UR - https://www.unboundmedicine.com/medline/citation/16202554/Na_+__K_+__ATPase_content_in_skeletal_muscle_of_dogs_with_pituitary_dependent_hyperadrenocorticism_ DB - PRIME DP - Unbound Medicine ER -