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Phenotypic analysis of alveolar macrophages and lymphocytes following allergen inhalation by atopic subjects with mild asthma.
Respir Med. 2006 May; 100(5):918-25.RM

Abstract

BACKGROUND

The airway inflammation associated with allergic asthma is initiated through a complex interaction of antigen-presenting cells (APC) and T lymphocytes resulting in the release of a cascade of cytokines regulating the progress of the allergic inflammatory response. In the present study the state of alveolar macrophage (AM) and T cell activation was investigated following induction of allergic airway inflammation in individuals with atopic asthma.

METHODS

Eleven individuals with mild, atopic asthma received cumulated allergen inhalations. Before and one day after challenge, bronchoalveolar lavage (BAL) was performed, and peripheral blood samples obtained. Ten healthy individuals served as controls. The expression of cell surface markers by BAL fluid AMs and T cells, and by blood T cells, was investigated by flow cytometry.

RESULTS

All patients developed early asthmatic reactions (EAR) with increased numbers of eosinophils and mast cells in BAL fluid following allergen challenge. After allergen challenge, patients had relatively fewer pulmonary CD4+ T cells expressing CD69 and HLA class II and also relatively fewer pulmonary CD8+ T cells expressing HLA class II, compared to before challenge. An increased quantitative expression of CD14 and CD86 was seen within the AM population following allergen challenge.

CONCLUSION

The results indicate a recruitment of non-activated, immature macrophages and CD4+ T cells to the airways as well as an altered phenotype pattern within the AM population following induction of allergic airway inflammation by allergen inhalation challenge in asthma.

Authors+Show Affiliations

Department of Medicine, Division of Respiratory Medicine, Karolinska University Hospital Solna and Karolinska Institutet, S-171 76 Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16202577

Citation

Lensmar, C, et al. "Phenotypic Analysis of Alveolar Macrophages and Lymphocytes Following Allergen Inhalation By Atopic Subjects With Mild Asthma." Respiratory Medicine, vol. 100, no. 5, 2006, pp. 918-25.
Lensmar C, Katchar K, Eklund A, et al. Phenotypic analysis of alveolar macrophages and lymphocytes following allergen inhalation by atopic subjects with mild asthma. Respir Med. 2006;100(5):918-25.
Lensmar, C., Katchar, K., Eklund, A., Grunewald, J., & Wahlström, J. (2006). Phenotypic analysis of alveolar macrophages and lymphocytes following allergen inhalation by atopic subjects with mild asthma. Respiratory Medicine, 100(5), 918-25.
Lensmar C, et al. Phenotypic Analysis of Alveolar Macrophages and Lymphocytes Following Allergen Inhalation By Atopic Subjects With Mild Asthma. Respir Med. 2006;100(5):918-25. PubMed PMID: 16202577.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic analysis of alveolar macrophages and lymphocytes following allergen inhalation by atopic subjects with mild asthma. AU - Lensmar,C, AU - Katchar,K, AU - Eklund,A, AU - Grunewald,J, AU - Wahlström,J, Y1 - 2005/10/03/ PY - 2005/03/22/received PY - 2005/07/21/accepted PY - 2005/10/6/pubmed PY - 2007/2/17/medline PY - 2005/10/6/entrez SP - 918 EP - 25 JF - Respiratory medicine JO - Respir Med VL - 100 IS - 5 N2 - BACKGROUND: The airway inflammation associated with allergic asthma is initiated through a complex interaction of antigen-presenting cells (APC) and T lymphocytes resulting in the release of a cascade of cytokines regulating the progress of the allergic inflammatory response. In the present study the state of alveolar macrophage (AM) and T cell activation was investigated following induction of allergic airway inflammation in individuals with atopic asthma. METHODS: Eleven individuals with mild, atopic asthma received cumulated allergen inhalations. Before and one day after challenge, bronchoalveolar lavage (BAL) was performed, and peripheral blood samples obtained. Ten healthy individuals served as controls. The expression of cell surface markers by BAL fluid AMs and T cells, and by blood T cells, was investigated by flow cytometry. RESULTS: All patients developed early asthmatic reactions (EAR) with increased numbers of eosinophils and mast cells in BAL fluid following allergen challenge. After allergen challenge, patients had relatively fewer pulmonary CD4+ T cells expressing CD69 and HLA class II and also relatively fewer pulmonary CD8+ T cells expressing HLA class II, compared to before challenge. An increased quantitative expression of CD14 and CD86 was seen within the AM population following allergen challenge. CONCLUSION: The results indicate a recruitment of non-activated, immature macrophages and CD4+ T cells to the airways as well as an altered phenotype pattern within the AM population following induction of allergic airway inflammation by allergen inhalation challenge in asthma. SN - 0954-6111 UR - https://www.unboundmedicine.com/medline/citation/16202577/Phenotypic_analysis_of_alveolar_macrophages_and_lymphocytes_following_allergen_inhalation_by_atopic_subjects_with_mild_asthma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0954-6111(05)00337-9 DB - PRIME DP - Unbound Medicine ER -