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Verapamil quantification in human plasma by liquid chromatography coupled to tandem mass spectrometry. An application for bioequivalence study.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Dec 05; 827(2):165-72.JC

Abstract

An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection (LC-MS/MS) was developed for the determination of Verapamil in human plasma using Metoprolol as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid-liquid extraction and chromatographed on a C(8) analytical column. The mobile phase consisted of methanol-water (70:30; v/v)+12 mM formic acid. The method had a chromatographic total run time of 3.5 min and was linear within the range 1.00-500 ng/mL. Detection was carried out on a Micromass Quattro Ultima tandem mass spectrometer by multiple reaction monitoring (MRM). The intra-run imprecision was less than 5.1% calculated from the quality control (QC) samples, and 16.3% from the limit of quantification (LOQ). The accuracy determined from QC samples were between 92.9 and 103.1%, and 95.2 and 115.3% from LOQ. Concerning the inter-batch analysis, the imprecision was less than 5.8% and 17.3% from QC samples and LOQ, respectively. The accuracy varied between 98.2 and 100.8% from QC and it was 103.1% from LOQ. The protocol herein described was employed in a bioequivalence study of two tablet formulations of Verapamil.

Authors+Show Affiliations

Department of Pharmacology, State University of Campinas, P.O. Box 6111, Campinas, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16203182

Citation

do C Borges, Ney Carter, et al. "Verapamil Quantification in Human Plasma By Liquid Chromatography Coupled to Tandem Mass Spectrometry. an Application for Bioequivalence Study." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 827, no. 2, 2005, pp. 165-72.
do C Borges NC, Mendes GD, Barrientos-Astigarraga RE, et al. Verapamil quantification in human plasma by liquid chromatography coupled to tandem mass spectrometry. An application for bioequivalence study. J Chromatogr B Analyt Technol Biomed Life Sci. 2005;827(2):165-72.
do C Borges, N. C., Mendes, G. D., Barrientos-Astigarraga, R. E., Galvinas, P., Oliveira, C. H., & De Nucci, G. (2005). Verapamil quantification in human plasma by liquid chromatography coupled to tandem mass spectrometry. An application for bioequivalence study. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 827(2), 165-72.
do C Borges NC, et al. Verapamil Quantification in Human Plasma By Liquid Chromatography Coupled to Tandem Mass Spectrometry. an Application for Bioequivalence Study. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Dec 5;827(2):165-72. PubMed PMID: 16203182.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Verapamil quantification in human plasma by liquid chromatography coupled to tandem mass spectrometry. An application for bioequivalence study. AU - do C Borges,Ney Carter, AU - Mendes,Gustavo D, AU - Barrientos-Astigarraga,Rafael E, AU - Galvinas,Paulo, AU - Oliveira,Celso H, AU - De Nucci,Gilberto, Y1 - 2005/10/03/ PY - 2003/11/25/received PY - 2005/06/27/revised PY - 2005/07/06/accepted PY - 2005/10/6/pubmed PY - 2006/2/4/medline PY - 2005/10/6/entrez SP - 165 EP - 72 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 827 IS - 2 N2 - An analytical method based on liquid chromatography with positive ion electrospray ionization (ESI) coupled to tandem mass spectrometry detection (LC-MS/MS) was developed for the determination of Verapamil in human plasma using Metoprolol as the internal standard. The analyte and internal standard were extracted from the plasma samples by liquid-liquid extraction and chromatographed on a C(8) analytical column. The mobile phase consisted of methanol-water (70:30; v/v)+12 mM formic acid. The method had a chromatographic total run time of 3.5 min and was linear within the range 1.00-500 ng/mL. Detection was carried out on a Micromass Quattro Ultima tandem mass spectrometer by multiple reaction monitoring (MRM). The intra-run imprecision was less than 5.1% calculated from the quality control (QC) samples, and 16.3% from the limit of quantification (LOQ). The accuracy determined from QC samples were between 92.9 and 103.1%, and 95.2 and 115.3% from LOQ. Concerning the inter-batch analysis, the imprecision was less than 5.8% and 17.3% from QC samples and LOQ, respectively. The accuracy varied between 98.2 and 100.8% from QC and it was 103.1% from LOQ. The protocol herein described was employed in a bioequivalence study of two tablet formulations of Verapamil. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16203182/Verapamil_quantification_in_human_plasma_by_liquid_chromatography_coupled_to_tandem_mass_spectrometry__An_application_for_bioequivalence_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(05)00489-7 DB - PRIME DP - Unbound Medicine ER -